The Crosstalk with CXCL10‐Rich Tumor‐Associated Mast Cells Fuels Pancreatic Cancer Progression and Immune Escape

Abstract Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease, necessitating approaches to improve prognosis. As the mediator of allergic process, mast cells have been found in various cancers and are associated with survival. However, the biological behaviors of tumor‐associated mast ce...

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Main Authors: Hanlin Yin, Qiangda Chen, Shanshan Gao, Sami Shoucair, Yuqi Xie, Joseph R. Habib, Taochen He, Wei Gan, Juan Wang, Lei Zhang, Huaxiang Xu, Chenye Shi, Junyi He, Wenquan Wang, Yun Jin, Michael G Goggins, Liang Liu, Wenhui Lou, Wenchuan Wu, Jun Yu, Ning Pu
Format: Article
Language:English
Published: Wiley 2025-04-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202417724
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author Hanlin Yin
Qiangda Chen
Shanshan Gao
Sami Shoucair
Yuqi Xie
Joseph R. Habib
Taochen He
Wei Gan
Juan Wang
Lei Zhang
Huaxiang Xu
Chenye Shi
Junyi He
Wenquan Wang
Yun Jin
Michael G Goggins
Liang Liu
Wenhui Lou
Wenchuan Wu
Jun Yu
Ning Pu
author_facet Hanlin Yin
Qiangda Chen
Shanshan Gao
Sami Shoucair
Yuqi Xie
Joseph R. Habib
Taochen He
Wei Gan
Juan Wang
Lei Zhang
Huaxiang Xu
Chenye Shi
Junyi He
Wenquan Wang
Yun Jin
Michael G Goggins
Liang Liu
Wenhui Lou
Wenchuan Wu
Jun Yu
Ning Pu
author_sort Hanlin Yin
collection DOAJ
description Abstract Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease, necessitating approaches to improve prognosis. As the mediator of allergic process, mast cells have been found in various cancers and are associated with survival. However, the biological behaviors of tumor‐associated mast cells (TAMCs) remain unclear. Herein, an excessive infiltration of TAMCs in PDAC is demonstrated, which apparently associated with poor survival in PDAC patients. PDAC cells are found to recruit CXCR2+ MCs into TME, and then inhibited MCs ferroptosis, and maintained their proliferation. Concomitantly, the tumor‐derived exosome miR‐188‐5p activated the PTEN/AKT/GSK3β signaling, further stabilized transcriptional factor ERG by inhibiting its ubiquitin degradation, and finally enhanced the transcription of cxcl10 within TAMCs. In reverse, TAMCs‐derived CXCL10 reversely promoted tumor epithelial‐mesenchymal transition and induced immunosuppressive tumor microenvironment by recruiting CXCR3+ Tregs. Sodium cromoglycate (SCG) is a membrane stabilizer for MCs and confirmed as an effective and widely used agent to block TAMCs‐derived CXCL10 and further sensitize the therapeutic efficacy of anti‐PD‐1 antibody plus gemcitabine for PDAC. These findings illuminate a critical and innovative crosstalk between TAMCs and PDAC cells that promote PDAC progression, and SCG sensitizes PDAC to the current immuno‐chemotherapy, which reveals its potential to be a valuable adjuvant for PDAC patients.
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spelling doaj-art-e0952a217c6b4a23aeafb384b840072b2025-08-20T03:09:08ZengWileyAdvanced Science2198-38442025-04-011214n/an/a10.1002/advs.202417724The Crosstalk with CXCL10‐Rich Tumor‐Associated Mast Cells Fuels Pancreatic Cancer Progression and Immune EscapeHanlin Yin0Qiangda Chen1Shanshan Gao2Sami Shoucair3Yuqi Xie4Joseph R. Habib5Taochen He6Wei Gan7Juan Wang8Lei Zhang9Huaxiang Xu10Chenye Shi11Junyi He12Wenquan Wang13Yun Jin14Michael G Goggins15Liang Liu16Wenhui Lou17Wenchuan Wu18Jun Yu19Ning Pu20Department of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Radiology Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartments of Medicine, Oncology and Surgery Johns Hopkins University School of Medicine Baltimore MD 21287 USADepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Surgery New York University School of Medicine and NYU‐Langone Medical Center New York NY 10016 USADepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaHANGZHOU CHEXMED TECHNOLOGY CO., LTD Hangzhou 310000 ChinaDepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Hepatobiliary and Pancreatic Surgery The First People's Hospital of Yunnan Province The Affiliated Hospital of Kunming University of Science and Technology Kunming 650500 ChinaDepartments of Medicine and Pathology The Sol Goldman Pancreatic Cancer Research Center Johns Hopkins University School of Medicine Baltimore MD 21287 USADepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaDepartments of Medicine, Oncology and Surgery Johns Hopkins University School of Medicine Baltimore MD 21287 USADepartment of Pancreatic Surgery Zhongshan Hospital Fudan University Shanghai 200032 ChinaAbstract Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease, necessitating approaches to improve prognosis. As the mediator of allergic process, mast cells have been found in various cancers and are associated with survival. However, the biological behaviors of tumor‐associated mast cells (TAMCs) remain unclear. Herein, an excessive infiltration of TAMCs in PDAC is demonstrated, which apparently associated with poor survival in PDAC patients. PDAC cells are found to recruit CXCR2+ MCs into TME, and then inhibited MCs ferroptosis, and maintained their proliferation. Concomitantly, the tumor‐derived exosome miR‐188‐5p activated the PTEN/AKT/GSK3β signaling, further stabilized transcriptional factor ERG by inhibiting its ubiquitin degradation, and finally enhanced the transcription of cxcl10 within TAMCs. In reverse, TAMCs‐derived CXCL10 reversely promoted tumor epithelial‐mesenchymal transition and induced immunosuppressive tumor microenvironment by recruiting CXCR3+ Tregs. Sodium cromoglycate (SCG) is a membrane stabilizer for MCs and confirmed as an effective and widely used agent to block TAMCs‐derived CXCL10 and further sensitize the therapeutic efficacy of anti‐PD‐1 antibody plus gemcitabine for PDAC. These findings illuminate a critical and innovative crosstalk between TAMCs and PDAC cells that promote PDAC progression, and SCG sensitizes PDAC to the current immuno‐chemotherapy, which reveals its potential to be a valuable adjuvant for PDAC patients.https://doi.org/10.1002/advs.202417724CXCL10immune escapepancreatic ductal adenocarcinomasodium cromoglycatetumor‐associated mast cell
spellingShingle Hanlin Yin
Qiangda Chen
Shanshan Gao
Sami Shoucair
Yuqi Xie
Joseph R. Habib
Taochen He
Wei Gan
Juan Wang
Lei Zhang
Huaxiang Xu
Chenye Shi
Junyi He
Wenquan Wang
Yun Jin
Michael G Goggins
Liang Liu
Wenhui Lou
Wenchuan Wu
Jun Yu
Ning Pu
The Crosstalk with CXCL10‐Rich Tumor‐Associated Mast Cells Fuels Pancreatic Cancer Progression and Immune Escape
Advanced Science
CXCL10
immune escape
pancreatic ductal adenocarcinoma
sodium cromoglycate
tumor‐associated mast cell
title The Crosstalk with CXCL10‐Rich Tumor‐Associated Mast Cells Fuels Pancreatic Cancer Progression and Immune Escape
title_full The Crosstalk with CXCL10‐Rich Tumor‐Associated Mast Cells Fuels Pancreatic Cancer Progression and Immune Escape
title_fullStr The Crosstalk with CXCL10‐Rich Tumor‐Associated Mast Cells Fuels Pancreatic Cancer Progression and Immune Escape
title_full_unstemmed The Crosstalk with CXCL10‐Rich Tumor‐Associated Mast Cells Fuels Pancreatic Cancer Progression and Immune Escape
title_short The Crosstalk with CXCL10‐Rich Tumor‐Associated Mast Cells Fuels Pancreatic Cancer Progression and Immune Escape
title_sort crosstalk with cxcl10 rich tumor associated mast cells fuels pancreatic cancer progression and immune escape
topic CXCL10
immune escape
pancreatic ductal adenocarcinoma
sodium cromoglycate
tumor‐associated mast cell
url https://doi.org/10.1002/advs.202417724
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