Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy
Methylglyoxal (MG) is an intermediate of glucose metabolism and the precursor of advanced glycation end products (AGEs) found in high levels in blood or tissue of diabetic patients. MG and AGEs are thought to play a major role in the pathogenesis of diabetic retinopathy. In order to determine if zeb...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2019/2746735 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850166319919398912 |
|---|---|
| author | Ying Li Yantao Zhao Shengmin Sang TinChung Leung |
| author_facet | Ying Li Yantao Zhao Shengmin Sang TinChung Leung |
| author_sort | Ying Li |
| collection | DOAJ |
| description | Methylglyoxal (MG) is an intermediate of glucose metabolism and the precursor of advanced glycation end products (AGEs) found in high levels in blood or tissue of diabetic patients. MG and AGEs are thought to play a major role in the pathogenesis of diabetic retinopathy. In order to determine if zebrafish is valuable to help us understand more about retinopathy, we evaluate if MG induces abnormal vascular change and angiogenesis in zebrafish in a short incubation period. We also used an inhibitor of VEGFR (PTK787) to explore the mechanistic role of VEGF in MG-induced pathogenesis. A transgenic Tg(flk1:GFP) zebrafish line was used, and the embryos were incubated with MG solution and in combination with glucose (to mimic hyperglycemia). Retinal vascular structure visible with fluorescence signal was imaged using fluorescence microscopy. The percentage of vascular area was calculated and found elevated in the MG treatment groups than that in the control group (p<0.01) which indicated increased angiogenesis induced by MG treatment. PTK787 blocked the proangiogenic effects of MG treatment. This study suggests that MG has a potential proangiogenic effect via VEGF signaling in the retina of zebrafish embryos. Therefore, this zebrafish model may be used to study neovascular retinopathy. |
| format | Article |
| id | doaj-art-e092a5a680b04804bc297cd78d9c863e |
| institution | OA Journals |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-e092a5a680b04804bc297cd78d9c863e2025-08-20T02:21:29ZengWileyJournal of Ophthalmology2090-004X2090-00582019-01-01201910.1155/2019/27467352746735Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular RetinopathyYing Li0Yantao Zhao1Shengmin Sang2TinChung Leung3Department of Geriatrics, Qi-Lu Hospital of Shandong University, Jinan, Shandong Province 250012, ChinaLaboratory for Functional Foods and Human Health, Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, Kannapolis, NC 28081, USALaboratory for Functional Foods and Human Health, Center for Excellence in Post-Harvest Technologies, North Carolina A&T State University, Kannapolis, NC 28081, USAJulius L. Chambers Biomedical/Biotechnology Research Institute, North Carolina Central University, Kannapolis, NC 28081, USAMethylglyoxal (MG) is an intermediate of glucose metabolism and the precursor of advanced glycation end products (AGEs) found in high levels in blood or tissue of diabetic patients. MG and AGEs are thought to play a major role in the pathogenesis of diabetic retinopathy. In order to determine if zebrafish is valuable to help us understand more about retinopathy, we evaluate if MG induces abnormal vascular change and angiogenesis in zebrafish in a short incubation period. We also used an inhibitor of VEGFR (PTK787) to explore the mechanistic role of VEGF in MG-induced pathogenesis. A transgenic Tg(flk1:GFP) zebrafish line was used, and the embryos were incubated with MG solution and in combination with glucose (to mimic hyperglycemia). Retinal vascular structure visible with fluorescence signal was imaged using fluorescence microscopy. The percentage of vascular area was calculated and found elevated in the MG treatment groups than that in the control group (p<0.01) which indicated increased angiogenesis induced by MG treatment. PTK787 blocked the proangiogenic effects of MG treatment. This study suggests that MG has a potential proangiogenic effect via VEGF signaling in the retina of zebrafish embryos. Therefore, this zebrafish model may be used to study neovascular retinopathy.http://dx.doi.org/10.1155/2019/2746735 |
| spellingShingle | Ying Li Yantao Zhao Shengmin Sang TinChung Leung Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy Journal of Ophthalmology |
| title | Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy |
| title_full | Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy |
| title_fullStr | Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy |
| title_full_unstemmed | Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy |
| title_short | Methylglyoxal-Induced Retinal Angiogenesis in Zebrafish Embryo: A Potential Animal Model of Neovascular Retinopathy |
| title_sort | methylglyoxal induced retinal angiogenesis in zebrafish embryo a potential animal model of neovascular retinopathy |
| url | http://dx.doi.org/10.1155/2019/2746735 |
| work_keys_str_mv | AT yingli methylglyoxalinducedretinalangiogenesisinzebrafishembryoapotentialanimalmodelofneovascularretinopathy AT yantaozhao methylglyoxalinducedretinalangiogenesisinzebrafishembryoapotentialanimalmodelofneovascularretinopathy AT shengminsang methylglyoxalinducedretinalangiogenesisinzebrafishembryoapotentialanimalmodelofneovascularretinopathy AT tinchungleung methylglyoxalinducedretinalangiogenesisinzebrafishembryoapotentialanimalmodelofneovascularretinopathy |