Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, China
Culicoides spp. (Diptera: Ceratopogonidae) are small biting midges, some of which are the vectors of arboviruses affecting livestock, i.e., African horse sickness virus (AHSV), bluetongue virus (BTV), and epizootic hemorrhagic disease virus (EHDV). Yunnan Province, located in southwestern China, has...
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EDP Sciences
2024-01-01
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| author | Duan Ying-Liang Li Zhan-Hong Bellis Glenn A. Li Le Liu Bing-Gang Wang Jian-Ping Liu Jian-Mei Liao De-Fang Zhu Jian-Bo |
| author_facet | Duan Ying-Liang Li Zhan-Hong Bellis Glenn A. Li Le Liu Bing-Gang Wang Jian-Ping Liu Jian-Mei Liao De-Fang Zhu Jian-Bo |
| author_sort | Duan Ying-Liang |
| collection | DOAJ |
| description | Culicoides spp. (Diptera: Ceratopogonidae) are small biting midges, some of which are the vectors of arboviruses affecting livestock, i.e., African horse sickness virus (AHSV), bluetongue virus (BTV), and epizootic hemorrhagic disease virus (EHDV). Yunnan Province, located in southwestern China, has a history of high prevalence of arboviruses. The diversity and abundance of Culicoides was observed between March 2022 and March 2023 on two cattle farms in Lufeng County of Yunnan Province, China and virus isolation and PCR detection were attempted from cattle blood and Culicoides spp. collected from the farms. Light trap collections contained 19 species of Culicoides belonging to 8 subgenera and one unplaced species group and were dominated by C. oxystoma (63.4%), C. imicola (16.2%), C. arakawae (13.4%), C. sp. near newsteadi (2.3%), and C. orientalis (1.7%). A total of 8,343 Culicoides were used for statistical analysis; from these collections 997 Culicoides specimens belonging to 10 species were screened for the presence of BTV, EHDV, Yunnan Orbivirus (YUOV), and Yongshan totivirus (YSToV) using reverse transcription quantitative PCR (RT-qPCR). One strain of YUOV was isolated from cattle blood and 7 isolates of YSToV were isolated from 5 different species of Culicoides. BTV and YSToV were detected from 2 and 3 pools of parous C. imicola specimens by RT-qPCR, respectively, which is the first report of a totivirus to be associated with Culicoides. Culicoides imicola is likely to be the major vector of Culicoides-borne arboviruses in Lufeng County, which is a relatively dry locality, and adult C. imicola may play a role of BTV overwintering. |
| format | Article |
| id | doaj-art-e0929c30f0a1425f82c770af6bfba7c8 |
| institution | OA Journals |
| issn | 1776-1042 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
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| spelling | doaj-art-e0929c30f0a1425f82c770af6bfba7c82025-08-20T02:31:08ZengEDP SciencesParasite1776-10422024-01-01317210.1051/parasite/2024072parasite240122Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, ChinaDuan Ying-Liang0https://orcid.org/0000-0002-7865-3525Li Zhan-Hong1Bellis Glenn A.2Li Le3Liu Bing-Gang4Wang Jian-Ping5Liu Jian-Mei6Liao De-Fang7Zhu Jian-Bo8Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary InstituteYunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary InstituteResearch Institute for the Environment and Livelihoods, Charles Darwin UniversityYunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary InstituteCenter for Animal Disease Control and PreventionCenter for Animal Disease Control and PreventionCenter for Animal Disease Control and PreventionYunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary InstituteYunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary InstituteCulicoides spp. (Diptera: Ceratopogonidae) are small biting midges, some of which are the vectors of arboviruses affecting livestock, i.e., African horse sickness virus (AHSV), bluetongue virus (BTV), and epizootic hemorrhagic disease virus (EHDV). Yunnan Province, located in southwestern China, has a history of high prevalence of arboviruses. The diversity and abundance of Culicoides was observed between March 2022 and March 2023 on two cattle farms in Lufeng County of Yunnan Province, China and virus isolation and PCR detection were attempted from cattle blood and Culicoides spp. collected from the farms. Light trap collections contained 19 species of Culicoides belonging to 8 subgenera and one unplaced species group and were dominated by C. oxystoma (63.4%), C. imicola (16.2%), C. arakawae (13.4%), C. sp. near newsteadi (2.3%), and C. orientalis (1.7%). A total of 8,343 Culicoides were used for statistical analysis; from these collections 997 Culicoides specimens belonging to 10 species were screened for the presence of BTV, EHDV, Yunnan Orbivirus (YUOV), and Yongshan totivirus (YSToV) using reverse transcription quantitative PCR (RT-qPCR). One strain of YUOV was isolated from cattle blood and 7 isolates of YSToV were isolated from 5 different species of Culicoides. BTV and YSToV were detected from 2 and 3 pools of parous C. imicola specimens by RT-qPCR, respectively, which is the first report of a totivirus to be associated with Culicoides. Culicoides imicola is likely to be the major vector of Culicoides-borne arboviruses in Lufeng County, which is a relatively dry locality, and adult C. imicola may play a role of BTV overwintering.https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite240122/parasite240122.htmlculicoidesbluetongue virusyunnan orbivirusyongshan totivirusoverwinter |
| spellingShingle | Duan Ying-Liang Li Zhan-Hong Bellis Glenn A. Li Le Liu Bing-Gang Wang Jian-Ping Liu Jian-Mei Liao De-Fang Zhu Jian-Bo Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, China Parasite culicoides bluetongue virus yunnan orbivirus yongshan totivirus overwinter |
| title | Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, China |
| title_full | Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, China |
| title_fullStr | Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, China |
| title_full_unstemmed | Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, China |
| title_short | Culicoides and midge-associated arboviruses on cattle farms in Yunnan Province, China |
| title_sort | culicoides and midge associated arboviruses on cattle farms in yunnan province china |
| topic | culicoides bluetongue virus yunnan orbivirus yongshan totivirus overwinter |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite240122/parasite240122.html |
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