WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells
Mutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neur...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/7848932 |
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| author | Abdullah J. Alshawaf Ana Antonic Efstratios Skafidas Dominic Chi-Hung Ng Mirella Dottori |
| author_facet | Abdullah J. Alshawaf Ana Antonic Efstratios Skafidas Dominic Chi-Hung Ng Mirella Dottori |
| author_sort | Abdullah J. Alshawaf |
| collection | DOAJ |
| description | Mutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neurospheres lacking WDR62 expression showed decreased expression of intermediate progenitor marker, TBR2, and also glial marker, S100β. In contrast, inhibition of c-Jun N-terminal kinase (JNK) signalling during hPSC neural differentiation induced upregulation of WDR62 with a corresponding increase in neural and glial progenitor markers, PAX6 and EAAT1, respectively. These findings may signify a role of WDR62 in specifying intermediate neural and glial progenitors during human pluripotent stem cell differentiation. |
| format | Article |
| id | doaj-art-e08eb42dbe164b9f9c9036340fab2ddc |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-e08eb42dbe164b9f9c9036340fab2ddc2025-08-20T03:26:09ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/78489327848932WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem CellsAbdullah J. Alshawaf0Ana Antonic1Efstratios Skafidas2Dominic Chi-Hung Ng3Mirella Dottori4Centre for Neural Engineering, The University of Melbourne, Carlton, VIC 3010, AustraliaCentre for Neural Engineering, The University of Melbourne, Carlton, VIC 3010, AustraliaCentre for Neural Engineering, The University of Melbourne, Carlton, VIC 3010, AustraliaSchool of Biomedical Science, Faculty of Medicine and Biomedical Sciences, University of Queensland, St Lucia, QLD 4072, AustraliaCentre for Neural Engineering, The University of Melbourne, Carlton, VIC 3010, AustraliaMutations in WD40-repeat protein 62 (WDR62) are commonly associated with primary microcephaly and other developmental cortical malformations. We used human pluripotent stem cells (hPSC) to examine WDR62 function during human neural differentiation and model early stages of human corticogenesis. Neurospheres lacking WDR62 expression showed decreased expression of intermediate progenitor marker, TBR2, and also glial marker, S100β. In contrast, inhibition of c-Jun N-terminal kinase (JNK) signalling during hPSC neural differentiation induced upregulation of WDR62 with a corresponding increase in neural and glial progenitor markers, PAX6 and EAAT1, respectively. These findings may signify a role of WDR62 in specifying intermediate neural and glial progenitors during human pluripotent stem cell differentiation.http://dx.doi.org/10.1155/2017/7848932 |
| spellingShingle | Abdullah J. Alshawaf Ana Antonic Efstratios Skafidas Dominic Chi-Hung Ng Mirella Dottori WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells Stem Cells International |
| title | WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells |
| title_full | WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells |
| title_fullStr | WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells |
| title_full_unstemmed | WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells |
| title_short | WDR62 Regulates Early Neural and Glial Progenitor Specification of Human Pluripotent Stem Cells |
| title_sort | wdr62 regulates early neural and glial progenitor specification of human pluripotent stem cells |
| url | http://dx.doi.org/10.1155/2017/7848932 |
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