Comparative analysis of Illumina, PacBio, and nanopore for 16S rRNA gene sequencing of rabbit’s gut microbiota

Comparative analysis of Illumina, PacBio, and nanopore for 16S rRNA gene sequencing of rabbit’s gut microbiota

This research aims to assess whether sequencing the full length of the 16S rRNA gene using PacBio HiFi sequencing and Oxford Nanopore Technology (ONT) platforms outperform Illumina MiSeq platform in providing detailed specie level insights. Moreover, it aims to compare the similarities in microbial...

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Bibliographic Details
Main Authors: Iliyass Biada, María Antonia Santacreu, Oscar González-Recio, Noelia Ibáñez-Escriche
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Microbiomes
Subjects:
16S rRNA
microbiota
Illumina
PacBio
nanopore
Online Access:https://www.frontiersin.org/articles/10.3389/frmbi.2025.1587712/full
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