Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.
<h4>Objective</h4>To undertake a comprehensive analysis of the biochemical tissue composition and passive biomechanical properties of ovine vagina and relate this to the histo-architecture at different reproductive stages as part of the establishment of a large preclinical animal model f...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093172&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850191818997628928 |
|---|---|
| author | Daniela Ulrich Sharon L Edwards Kai Su Jacinta F White John A M Ramshaw Graham Jenkin Jan Deprest Anna Rosamilia Jerome A Werkmeister Caroline E Gargett |
| author_facet | Daniela Ulrich Sharon L Edwards Kai Su Jacinta F White John A M Ramshaw Graham Jenkin Jan Deprest Anna Rosamilia Jerome A Werkmeister Caroline E Gargett |
| author_sort | Daniela Ulrich |
| collection | DOAJ |
| description | <h4>Objective</h4>To undertake a comprehensive analysis of the biochemical tissue composition and passive biomechanical properties of ovine vagina and relate this to the histo-architecture at different reproductive stages as part of the establishment of a large preclinical animal model for evaluating regenerative medicine approaches for surgical treatment of pelvic organ prolapse.<h4>Methods</h4>Vaginal tissue was collected from virgin (n = 3), parous (n = 6) and pregnant sheep (n = 6; mean gestation; 132 d; term = 145 d). Tissue histology was analyzed using H+E and Masson's Trichrome staining. Biochemical analysis of the extracellular matrix proteins used a hydroxyproline assay to quantify total collagen, SDS PAGE to measure collagen III/I+III ratios, dimethylmethylene blue to quantify glycosaminoglycans and amino acid analysis to quantify elastin. Uniaxial tensiometry was used to determine the Young's modulus, maximum stress and strain, and permanent strain following cyclic loading.<h4>Results</h4>Vaginal tissue of virgin sheep had the lowest total collagen content and permanent strain. Parous tissue had the highest total collagen and lowest elastin content with concomitant high maximum stress. In contrast, pregnant sheep had the highest elastin and lowest collagen contents, and thickest smooth muscle layer, which was associated with low maximum stress and poor dimensional recovery following repetitive loading.<h4>Conclusion</h4>Pregnant ovine vagina was the most extensible, but the weakest tissue, whereas parous and virgin tissues were strong and elastic. Pregnancy had the greatest impact on tissue composition and biomechanical properties, compatible with significant tissue remodeling as demonstrated in other species. Biochemical changes in tissue protein composition coincide with these altered biomechanical properties. |
| format | Article |
| id | doaj-art-e08138f39ad74a979a080dff3ec03fd9 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-e08138f39ad74a979a080dff3ec03fd92025-08-20T02:14:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9317210.1371/journal.pone.0093172Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina.Daniela UlrichSharon L EdwardsKai SuJacinta F WhiteJohn A M RamshawGraham JenkinJan DeprestAnna RosamiliaJerome A WerkmeisterCaroline E Gargett<h4>Objective</h4>To undertake a comprehensive analysis of the biochemical tissue composition and passive biomechanical properties of ovine vagina and relate this to the histo-architecture at different reproductive stages as part of the establishment of a large preclinical animal model for evaluating regenerative medicine approaches for surgical treatment of pelvic organ prolapse.<h4>Methods</h4>Vaginal tissue was collected from virgin (n = 3), parous (n = 6) and pregnant sheep (n = 6; mean gestation; 132 d; term = 145 d). Tissue histology was analyzed using H+E and Masson's Trichrome staining. Biochemical analysis of the extracellular matrix proteins used a hydroxyproline assay to quantify total collagen, SDS PAGE to measure collagen III/I+III ratios, dimethylmethylene blue to quantify glycosaminoglycans and amino acid analysis to quantify elastin. Uniaxial tensiometry was used to determine the Young's modulus, maximum stress and strain, and permanent strain following cyclic loading.<h4>Results</h4>Vaginal tissue of virgin sheep had the lowest total collagen content and permanent strain. Parous tissue had the highest total collagen and lowest elastin content with concomitant high maximum stress. In contrast, pregnant sheep had the highest elastin and lowest collagen contents, and thickest smooth muscle layer, which was associated with low maximum stress and poor dimensional recovery following repetitive loading.<h4>Conclusion</h4>Pregnant ovine vagina was the most extensible, but the weakest tissue, whereas parous and virgin tissues were strong and elastic. Pregnancy had the greatest impact on tissue composition and biomechanical properties, compatible with significant tissue remodeling as demonstrated in other species. Biochemical changes in tissue protein composition coincide with these altered biomechanical properties.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093172&type=printable |
| spellingShingle | Daniela Ulrich Sharon L Edwards Kai Su Jacinta F White John A M Ramshaw Graham Jenkin Jan Deprest Anna Rosamilia Jerome A Werkmeister Caroline E Gargett Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina. PLoS ONE |
| title | Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina. |
| title_full | Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina. |
| title_fullStr | Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina. |
| title_full_unstemmed | Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina. |
| title_short | Influence of reproductive status on tissue composition and biomechanical properties of ovine vagina. |
| title_sort | influence of reproductive status on tissue composition and biomechanical properties of ovine vagina |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0093172&type=printable |
| work_keys_str_mv | AT danielaulrich influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT sharonledwards influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT kaisu influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT jacintafwhite influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT johnamramshaw influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT grahamjenkin influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT jandeprest influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT annarosamilia influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT jeromeawerkmeister influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina AT carolineegargett influenceofreproductivestatusontissuecompositionandbiomechanicalpropertiesofovinevagina |