A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis
Abstract Plant development, defense, and many physiological processes rely on the endosomal sorting complex required for transport (ESCRT) machinery to control the homeostasis of membrane proteins by selective vacuolar degradation. Although ESCRT core components are conserved among higher eukaryotes...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-05913-y |
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| _version_ | 1850125703798849536 |
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| author | Jinbo Shen Qiong Zhao Xiangfeng Wang Caiji Gao Ying Zhu Yonglun Zeng Liwen Jiang |
| author_facet | Jinbo Shen Qiong Zhao Xiangfeng Wang Caiji Gao Ying Zhu Yonglun Zeng Liwen Jiang |
| author_sort | Jinbo Shen |
| collection | DOAJ |
| description | Abstract Plant development, defense, and many physiological processes rely on the endosomal sorting complex required for transport (ESCRT) machinery to control the homeostasis of membrane proteins by selective vacuolar degradation. Although ESCRT core components are conserved among higher eukaryotes, the regulators that control the function of the ESCRT machinery remain elusive. We recently identified a plant-specific ESCRT component, FREE1, that is essential for multivesicular body/prevacuolar compartment (MVB/PVC) biogenesis and vacuolar sorting of membrane proteins. Here we identify a plant-specific Bro1-domain protein BRAF, which regulates FREE1 recruitment to the MVB/PVC membrane by competitively binding to the ESCRT-I component Vps23. Altogether, we have successfully identified a role for BRAF, whose function as a unique evolutionary ESCRT regulator in orchestrating intraluminal vesicle formation in MVB/PVCs and the sorting of membrane proteins for degradation in plants makes it an important regulatory mechanism underlying the ESCRT machinery in higher eukaryotes. |
| format | Article |
| id | doaj-art-e07d09322bbf42ac97b3fb0813458d2b |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2018-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e07d09322bbf42ac97b3fb0813458d2b2025-08-20T02:34:04ZengNature PortfolioNature Communications2041-17232018-09-019111510.1038/s41467-018-05913-yA plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasisJinbo Shen0Qiong Zhao1Xiangfeng Wang2Caiji Gao3Ying Zhu4Yonglun Zeng5Liwen Jiang6Centre for Cell & Developmental Biology, State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong KongCentre for Cell & Developmental Biology, State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong KongCentre for Cell & Developmental Biology, State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong KongCentre for Cell & Developmental Biology, State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong KongCentre for Cell & Developmental Biology, State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong KongCentre for Cell & Developmental Biology, State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong KongCentre for Cell & Developmental Biology, State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong KongAbstract Plant development, defense, and many physiological processes rely on the endosomal sorting complex required for transport (ESCRT) machinery to control the homeostasis of membrane proteins by selective vacuolar degradation. Although ESCRT core components are conserved among higher eukaryotes, the regulators that control the function of the ESCRT machinery remain elusive. We recently identified a plant-specific ESCRT component, FREE1, that is essential for multivesicular body/prevacuolar compartment (MVB/PVC) biogenesis and vacuolar sorting of membrane proteins. Here we identify a plant-specific Bro1-domain protein BRAF, which regulates FREE1 recruitment to the MVB/PVC membrane by competitively binding to the ESCRT-I component Vps23. Altogether, we have successfully identified a role for BRAF, whose function as a unique evolutionary ESCRT regulator in orchestrating intraluminal vesicle formation in MVB/PVCs and the sorting of membrane proteins for degradation in plants makes it an important regulatory mechanism underlying the ESCRT machinery in higher eukaryotes.https://doi.org/10.1038/s41467-018-05913-y |
| spellingShingle | Jinbo Shen Qiong Zhao Xiangfeng Wang Caiji Gao Ying Zhu Yonglun Zeng Liwen Jiang A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis Nature Communications |
| title | A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis |
| title_full | A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis |
| title_fullStr | A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis |
| title_full_unstemmed | A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis |
| title_short | A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis |
| title_sort | plant bro1 domain protein braf regulates multivesicular body biogenesis and membrane protein homeostasis |
| url | https://doi.org/10.1038/s41467-018-05913-y |
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