Assessment of enamel demineralization, shear bond strength, and failure mode following the use of orthodontic adhesive containing silver nanoparticles: an in-vitro study

ObjectiveThis study evaluated enamel demineralization, shear bond strength, and failure modes associated with orthodontic adhesives containing silver nanoparticles (AgNPs).MethodsForty-eight extracted human premolar teeth were selected and evenly divided into two groups based on the intended assessm...

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Main Authors: Naif Almosa, Ahmed Alsaleh, Alhanouf Alnowaiser, Nada Alhogail, Abdalaziz Alsuliman, Latifa Alswailem, Rahaf Zawawi, Syed Rashid Habib
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Materials
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Online Access:https://www.frontiersin.org/articles/10.3389/fmats.2025.1598515/full
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author Naif Almosa
Ahmed Alsaleh
Alhanouf Alnowaiser
Nada Alhogail
Abdalaziz Alsuliman
Latifa Alswailem
Rahaf Zawawi
Syed Rashid Habib
author_facet Naif Almosa
Ahmed Alsaleh
Alhanouf Alnowaiser
Nada Alhogail
Abdalaziz Alsuliman
Latifa Alswailem
Rahaf Zawawi
Syed Rashid Habib
author_sort Naif Almosa
collection DOAJ
description ObjectiveThis study evaluated enamel demineralization, shear bond strength, and failure modes associated with orthodontic adhesives containing silver nanoparticles (AgNPs).MethodsForty-eight extracted human premolar teeth were selected and evenly divided into two groups based on the intended assessments, with 28 specimens in each group: Part I focused on enamel demineralization depth, and Part II addressed shear bond strength and failure modes. Specimens were prepared, mounted, and bonded using two adhesive systems: Group A used a conventional adhesive system without AgNPs, while Group B employed a conventional adhesive system mixed with AgNPs. In Part I, specimens underwent artificial demineralization at pH 4.5°C and 37°C for 7 days, followed by buccolingual sectioning. The depth of demineralization was measured using a scanning electron microscope and analyzed with ImageJ software. In Part II, shear bond strength was assessed using a universal testing machine, followed by failure mode evaluation using a stereomicroscope.ResultsResults showed that Group A exhibited a significantly greater demineralization depth compared to Group B (p = 0.000). However, no significant difference was observed between overall location levels (p = 0.093). Additionally, Group B demonstrated significantly higher shear bond strength (p = 0.000) and a more uniform distribution of failure modes compared to Group A.ConclusionsThese findings suggest that incorporating silver nanoparticles into orthodontic adhesives reduces demineralization depth and enhances shear bond strength compared to conventional adhesive systems. In contrast, conventional adhesives were associated with a higher incidence of adhesive mode failure.
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spelling doaj-art-e079903539e74ac18b3e175cda5e3d2d2025-08-20T03:07:54ZengFrontiers Media S.A.Frontiers in Materials2296-80162025-06-011210.3389/fmats.2025.15985151598515Assessment of enamel demineralization, shear bond strength, and failure mode following the use of orthodontic adhesive containing silver nanoparticles: an in-vitro studyNaif Almosa0Ahmed Alsaleh1Alhanouf Alnowaiser2Nada Alhogail3Abdalaziz Alsuliman4Latifa Alswailem5Rahaf Zawawi6Syed Rashid Habib7Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaDepartment of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaSaudi Board of Orthodontics and Dentofacial Orthopedics Program, Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaSaudi Board of Orthodontics and Dentofacial Orthopedics Program, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, Riyadh, Saudi ArabiaGeneral dentist, College of Dentistry/ King Saud University, Riyadh, Saudi ArabiaGeneral dentist, College of Dentistry/ King Saud University, Riyadh, Saudi ArabiaIndependent Researcher, Riyadh, Saudi ArabiaDepartment of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaObjectiveThis study evaluated enamel demineralization, shear bond strength, and failure modes associated with orthodontic adhesives containing silver nanoparticles (AgNPs).MethodsForty-eight extracted human premolar teeth were selected and evenly divided into two groups based on the intended assessments, with 28 specimens in each group: Part I focused on enamel demineralization depth, and Part II addressed shear bond strength and failure modes. Specimens were prepared, mounted, and bonded using two adhesive systems: Group A used a conventional adhesive system without AgNPs, while Group B employed a conventional adhesive system mixed with AgNPs. In Part I, specimens underwent artificial demineralization at pH 4.5°C and 37°C for 7 days, followed by buccolingual sectioning. The depth of demineralization was measured using a scanning electron microscope and analyzed with ImageJ software. In Part II, shear bond strength was assessed using a universal testing machine, followed by failure mode evaluation using a stereomicroscope.ResultsResults showed that Group A exhibited a significantly greater demineralization depth compared to Group B (p = 0.000). However, no significant difference was observed between overall location levels (p = 0.093). Additionally, Group B demonstrated significantly higher shear bond strength (p = 0.000) and a more uniform distribution of failure modes compared to Group A.ConclusionsThese findings suggest that incorporating silver nanoparticles into orthodontic adhesives reduces demineralization depth and enhances shear bond strength compared to conventional adhesive systems. In contrast, conventional adhesives were associated with a higher incidence of adhesive mode failure.https://www.frontiersin.org/articles/10.3389/fmats.2025.1598515/fullorthodontic adhesivesilver nanoparticlesshear bond strengthmode of failureenamel demineralization
spellingShingle Naif Almosa
Ahmed Alsaleh
Alhanouf Alnowaiser
Nada Alhogail
Abdalaziz Alsuliman
Latifa Alswailem
Rahaf Zawawi
Syed Rashid Habib
Assessment of enamel demineralization, shear bond strength, and failure mode following the use of orthodontic adhesive containing silver nanoparticles: an in-vitro study
Frontiers in Materials
orthodontic adhesive
silver nanoparticles
shear bond strength
mode of failure
enamel demineralization
title Assessment of enamel demineralization, shear bond strength, and failure mode following the use of orthodontic adhesive containing silver nanoparticles: an in-vitro study
title_full Assessment of enamel demineralization, shear bond strength, and failure mode following the use of orthodontic adhesive containing silver nanoparticles: an in-vitro study
title_fullStr Assessment of enamel demineralization, shear bond strength, and failure mode following the use of orthodontic adhesive containing silver nanoparticles: an in-vitro study
title_full_unstemmed Assessment of enamel demineralization, shear bond strength, and failure mode following the use of orthodontic adhesive containing silver nanoparticles: an in-vitro study
title_short Assessment of enamel demineralization, shear bond strength, and failure mode following the use of orthodontic adhesive containing silver nanoparticles: an in-vitro study
title_sort assessment of enamel demineralization shear bond strength and failure mode following the use of orthodontic adhesive containing silver nanoparticles an in vitro study
topic orthodontic adhesive
silver nanoparticles
shear bond strength
mode of failure
enamel demineralization
url https://www.frontiersin.org/articles/10.3389/fmats.2025.1598515/full
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