Thermo-responsive triple-function nanotransporter for efficient chemo-photothermal therapy of multidrug-resistant bacterial infection

Abstract New strategies with high antimicrobial efficacy against multidrug-resistant bacteria are urgently desired. Herein, we describe a smart triple-functional nanostructure, namely TRIDENT (Thermo-Responsive-Inspired Drug-Delivery Nano-Transporter), for reliable bacterial eradication. The robust...

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Main Authors: Guangchao Qing, Xianxian Zhao, Ningqiang Gong, Jing Chen, Xianlei Li, Yaling Gan, Yongchao Wang, Zhen Zhang, Yuxuan Zhang, Weisheng Guo, Yang Luo, Xing-Jie Liang
Format: Article
Language:English
Published: Nature Portfolio 2019-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-12313-3
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author Guangchao Qing
Xianxian Zhao
Ningqiang Gong
Jing Chen
Xianlei Li
Yaling Gan
Yongchao Wang
Zhen Zhang
Yuxuan Zhang
Weisheng Guo
Yang Luo
Xing-Jie Liang
author_facet Guangchao Qing
Xianxian Zhao
Ningqiang Gong
Jing Chen
Xianlei Li
Yaling Gan
Yongchao Wang
Zhen Zhang
Yuxuan Zhang
Weisheng Guo
Yang Luo
Xing-Jie Liang
author_sort Guangchao Qing
collection DOAJ
description Abstract New strategies with high antimicrobial efficacy against multidrug-resistant bacteria are urgently desired. Herein, we describe a smart triple-functional nanostructure, namely TRIDENT (Thermo-Responsive-Inspired Drug-Delivery Nano-Transporter), for reliable bacterial eradication. The robust antibacterial effectiveness is attributed to the integrated fluorescence monitoring and synergistic chemo-photothermal killing. We notice that temperature rises generated by near-infrared irradiation did not only melt the nanotransporter via a phase change mechanism, but also irreversibly damaged bacterial membranes to facilitate imipenem permeation, thus interfering with cell wall biosynthesis and eventually leading to rapid bacterial death. Both in vitro and in vivo evidence demonstrate that even low doses of imipenem-encapsulated TRIDENT could eradicate clinical methicillin-resistant Staphylococcus aureus, whereas imipenem alone had limited effect. Due to rapid recovery of infected sites and good biosafety we envision a universal antimicrobial platform to fight against multidrug-resistant or extremely drug-resistant bacteria.
format Article
id doaj-art-e07913e02ed249c4b04fd42124a95546
institution OA Journals
issn 2041-1723
language English
publishDate 2019-09-01
publisher Nature Portfolio
record_format Article
series Nature Communications
spelling doaj-art-e07913e02ed249c4b04fd42124a955462025-08-20T02:10:54ZengNature PortfolioNature Communications2041-17232019-09-0110111210.1038/s41467-019-12313-3Thermo-responsive triple-function nanotransporter for efficient chemo-photothermal therapy of multidrug-resistant bacterial infectionGuangchao Qing0Xianxian Zhao1Ningqiang Gong2Jing Chen3Xianlei Li4Yaling Gan5Yongchao Wang6Zhen Zhang7Yuxuan Zhang8Weisheng Guo9Yang Luo10Xing-Jie Liang11Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, College of Bioengineering, Chongqing UniversityDepartment of Clinical Laboratory Medicine, Southwest Hospital, Army Medical UniversityChinese Academy of Sciences (CAS) Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of ChinaChinese Academy of Sciences (CAS) Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of ChinaChinese Academy of Sciences (CAS) Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of ChinaChinese Academy of Sciences (CAS) Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of ChinaChinese Academy of Sciences (CAS) Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of ChinaChinese Academy of Sciences (CAS) Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of ChinaChinese Academy of Sciences (CAS) Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of ChinaTranslational Medicine Center, Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & The Second Affiliated Hospital, Guangzhou Medical UniversityKey Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, College of Bioengineering, Chongqing UniversityChinese Academy of Sciences (CAS) Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of ChinaAbstract New strategies with high antimicrobial efficacy against multidrug-resistant bacteria are urgently desired. Herein, we describe a smart triple-functional nanostructure, namely TRIDENT (Thermo-Responsive-Inspired Drug-Delivery Nano-Transporter), for reliable bacterial eradication. The robust antibacterial effectiveness is attributed to the integrated fluorescence monitoring and synergistic chemo-photothermal killing. We notice that temperature rises generated by near-infrared irradiation did not only melt the nanotransporter via a phase change mechanism, but also irreversibly damaged bacterial membranes to facilitate imipenem permeation, thus interfering with cell wall biosynthesis and eventually leading to rapid bacterial death. Both in vitro and in vivo evidence demonstrate that even low doses of imipenem-encapsulated TRIDENT could eradicate clinical methicillin-resistant Staphylococcus aureus, whereas imipenem alone had limited effect. Due to rapid recovery of infected sites and good biosafety we envision a universal antimicrobial platform to fight against multidrug-resistant or extremely drug-resistant bacteria.https://doi.org/10.1038/s41467-019-12313-3
spellingShingle Guangchao Qing
Xianxian Zhao
Ningqiang Gong
Jing Chen
Xianlei Li
Yaling Gan
Yongchao Wang
Zhen Zhang
Yuxuan Zhang
Weisheng Guo
Yang Luo
Xing-Jie Liang
Thermo-responsive triple-function nanotransporter for efficient chemo-photothermal therapy of multidrug-resistant bacterial infection
Nature Communications
title Thermo-responsive triple-function nanotransporter for efficient chemo-photothermal therapy of multidrug-resistant bacterial infection
title_full Thermo-responsive triple-function nanotransporter for efficient chemo-photothermal therapy of multidrug-resistant bacterial infection
title_fullStr Thermo-responsive triple-function nanotransporter for efficient chemo-photothermal therapy of multidrug-resistant bacterial infection
title_full_unstemmed Thermo-responsive triple-function nanotransporter for efficient chemo-photothermal therapy of multidrug-resistant bacterial infection
title_short Thermo-responsive triple-function nanotransporter for efficient chemo-photothermal therapy of multidrug-resistant bacterial infection
title_sort thermo responsive triple function nanotransporter for efficient chemo photothermal therapy of multidrug resistant bacterial infection
url https://doi.org/10.1038/s41467-019-12313-3
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