Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean
Abstract The beta-rhizobial strain Paraburkholderia phymatum STM815T is noteworthy for its wide host range in nodulating legumes, primarily mimosoids (over 50 different species) but also some papilionoids. It cannot, however, nodulate soybean (Glycine max [L.] Merr.), one of the world’s most importa...
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Nature Portfolio
2024-12-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-07385-x |
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| author | Paula Bellés-Sancho Daphné Golaz Sarah Paszti Alessandra Vitale Yilei Liu Aurélien Bailly Leo Eberl Euan K. James Gabriella Pessi |
| author_facet | Paula Bellés-Sancho Daphné Golaz Sarah Paszti Alessandra Vitale Yilei Liu Aurélien Bailly Leo Eberl Euan K. James Gabriella Pessi |
| author_sort | Paula Bellés-Sancho |
| collection | DOAJ |
| description | Abstract The beta-rhizobial strain Paraburkholderia phymatum STM815T is noteworthy for its wide host range in nodulating legumes, primarily mimosoids (over 50 different species) but also some papilionoids. It cannot, however, nodulate soybean (Glycine max [L.] Merr.), one of the world’s most important crops. Here, we constructed a highly saturated genome-wide transposon library of a P. phymatum strain and employed a transposon sequencing (Tn-seq) approach to investigate the underlying genetic mechanisms of symbiotic incompatibility between P. phymatum and soybean. Soybean seedlings inoculated with the P. phymatum Tn-seq library display nodules on the roots that are mainly occupied by different mutants in a gene, nodS, coding for a methyltransferase involved in the biosynthesis of nodulation factors. The construction of a nodS deletion strain and a complemented mutant confirms that nodS is responsible for the nodulation-incompatibility of P. phymatum with soybean. Moreover, infection tests with different host plants reveal that NodS is necessary for optimal nodulation of common bean (Phaseolus vulgaris), but it is not required for nodulation of its natural host Mimosa pudica. In conclusion, our results suggest that NodS is involved in determining nodulation specificity of P. phymatum. |
| format | Article |
| id | doaj-art-e07412a428604e0ab3b4ab195346d617 |
| institution | DOAJ |
| issn | 2399-3642 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-e07412a428604e0ab3b4ab195346d6172025-08-20T02:43:25ZengNature PortfolioCommunications Biology2399-36422024-12-017111310.1038/s42003-024-07385-xTn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybeanPaula Bellés-Sancho0Daphné Golaz1Sarah Paszti2Alessandra Vitale3Yilei Liu4Aurélien Bailly5Leo Eberl6Euan K. James7Gabriella Pessi8Department of Plant and Microbial Biology, University of ZurichDepartment of Plant and Microbial Biology, University of ZurichDepartment of Plant and Microbial Biology, University of ZurichDepartment of Plant and Microbial Biology, University of ZurichDepartment of Plant and Microbial Biology, University of ZurichDepartment of Plant and Microbial Biology, University of ZurichDepartment of Plant and Microbial Biology, University of ZurichEcological Sciences, The James Hutton InstituteDepartment of Plant and Microbial Biology, University of ZurichAbstract The beta-rhizobial strain Paraburkholderia phymatum STM815T is noteworthy for its wide host range in nodulating legumes, primarily mimosoids (over 50 different species) but also some papilionoids. It cannot, however, nodulate soybean (Glycine max [L.] Merr.), one of the world’s most important crops. Here, we constructed a highly saturated genome-wide transposon library of a P. phymatum strain and employed a transposon sequencing (Tn-seq) approach to investigate the underlying genetic mechanisms of symbiotic incompatibility between P. phymatum and soybean. Soybean seedlings inoculated with the P. phymatum Tn-seq library display nodules on the roots that are mainly occupied by different mutants in a gene, nodS, coding for a methyltransferase involved in the biosynthesis of nodulation factors. The construction of a nodS deletion strain and a complemented mutant confirms that nodS is responsible for the nodulation-incompatibility of P. phymatum with soybean. Moreover, infection tests with different host plants reveal that NodS is necessary for optimal nodulation of common bean (Phaseolus vulgaris), but it is not required for nodulation of its natural host Mimosa pudica. In conclusion, our results suggest that NodS is involved in determining nodulation specificity of P. phymatum.https://doi.org/10.1038/s42003-024-07385-x |
| spellingShingle | Paula Bellés-Sancho Daphné Golaz Sarah Paszti Alessandra Vitale Yilei Liu Aurélien Bailly Leo Eberl Euan K. James Gabriella Pessi Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean Communications Biology |
| title | Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean |
| title_full | Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean |
| title_fullStr | Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean |
| title_full_unstemmed | Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean |
| title_short | Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean |
| title_sort | tn seq profiling reveals that nods of the beta rhizobium paraburkholderia phymatum is detrimental for nodulating soybean |
| url | https://doi.org/10.1038/s42003-024-07385-x |
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