Molecular Epidemiology of Dengue Viruses in Lao People’s Democratic Republic, 2020–2023

Dengue fever is a widespread mosquito-borne viral disease caused by infections with dengue virus (DENV). Since its initial detection in 1979, the disease has posed a significant public health threat to the Lao People’s Democratic Republic (Lao PDR). Surveillance is crucial for understanding the circ...

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Main Authors: Cécile Troupin, Kedkeo Intavong, Somphavanh Somlor, Souksakhone Viengphouthong, Sitsana Keosenhom, Thep Aksone Chindavong, Phaithong Bounmany, Longthor Vachouaxiong, Thonglakhone Xaybounsou, Chittaphone Vanhnollat, Phonepadith Khattignavong, Darouny Phonekeo, Bouaphanh Khamphaphongphane, Phonepadith Xangsayarath, Vincent Lacoste, Philippe Buchy, Gary Wong
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/2/318
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author Cécile Troupin
Kedkeo Intavong
Somphavanh Somlor
Souksakhone Viengphouthong
Sitsana Keosenhom
Thep Aksone Chindavong
Phaithong Bounmany
Longthor Vachouaxiong
Thonglakhone Xaybounsou
Chittaphone Vanhnollat
Phonepadith Khattignavong
Darouny Phonekeo
Bouaphanh Khamphaphongphane
Phonepadith Xangsayarath
Vincent Lacoste
Philippe Buchy
Gary Wong
author_facet Cécile Troupin
Kedkeo Intavong
Somphavanh Somlor
Souksakhone Viengphouthong
Sitsana Keosenhom
Thep Aksone Chindavong
Phaithong Bounmany
Longthor Vachouaxiong
Thonglakhone Xaybounsou
Chittaphone Vanhnollat
Phonepadith Khattignavong
Darouny Phonekeo
Bouaphanh Khamphaphongphane
Phonepadith Xangsayarath
Vincent Lacoste
Philippe Buchy
Gary Wong
author_sort Cécile Troupin
collection DOAJ
description Dengue fever is a widespread mosquito-borne viral disease caused by infections with dengue virus (DENV). Since its initial detection in 1979, the disease has posed a significant public health threat to the Lao People’s Democratic Republic (Lao PDR). Surveillance is crucial for understanding the circulation of DENV in endemic regions and identifying potential hot spots with higher-than-expected case numbers of dengue fever. In this study, we present the results from our surveillance activities in the Lao PDR spanning 2020–2023. While quarantine restrictions from the COVID-19 pandemic posed substantial disruptions to performing DENV surveillance, over 8800 samples were tested during this period, with a positive rate of close to 60%. Cases were reported from all three regions (northern, Central, and southern) of the Lao PDR. Three circulating serotypes (DENV-1, DENV-2, and DENV-4) were detected, with DENV-1 dominant in 2021 and 2022, while DENV-2 was dominant in 2020 and 2023. Phylogenetic analyses suggest that the genotypes of DENV-1, DENV-2, and DENV-4 were closely related to corresponding isolates from neighboring countries. These findings provide an update on the nature of DENV cases detected in the Lao PDR and underscore the critical importance of sustaining a robust surveillance network to track infections.
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spelling doaj-art-e06ea015d7ae4b04930ef09242d841d92025-08-20T03:12:05ZengMDPI AGMicroorganisms2076-26072025-02-0113231810.3390/microorganisms13020318Molecular Epidemiology of Dengue Viruses in Lao People’s Democratic Republic, 2020–2023Cécile Troupin0Kedkeo Intavong1Somphavanh Somlor2Souksakhone Viengphouthong3Sitsana Keosenhom4Thep Aksone Chindavong5Phaithong Bounmany6Longthor Vachouaxiong7Thonglakhone Xaybounsou8Chittaphone Vanhnollat9Phonepadith Khattignavong10Darouny Phonekeo11Bouaphanh Khamphaphongphane12Phonepadith Xangsayarath13Vincent Lacoste14Philippe Buchy15Gary Wong16Virology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosParasitology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosAdministration Department, Institut Pasteur du Laos, Vientiane 01030, LaosNational Center for Laboratory and Epidemiology, Ministry of Health, Vientiane 01030, LaosDepartment of Communicable Disease Control, Ministry of Health, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosAdministration Department, Institut Pasteur du Laos, Vientiane 01030, LaosVirology Laboratory, Institut Pasteur du Laos, Vientiane 01030, LaosDengue fever is a widespread mosquito-borne viral disease caused by infections with dengue virus (DENV). Since its initial detection in 1979, the disease has posed a significant public health threat to the Lao People’s Democratic Republic (Lao PDR). Surveillance is crucial for understanding the circulation of DENV in endemic regions and identifying potential hot spots with higher-than-expected case numbers of dengue fever. In this study, we present the results from our surveillance activities in the Lao PDR spanning 2020–2023. While quarantine restrictions from the COVID-19 pandemic posed substantial disruptions to performing DENV surveillance, over 8800 samples were tested during this period, with a positive rate of close to 60%. Cases were reported from all three regions (northern, Central, and southern) of the Lao PDR. Three circulating serotypes (DENV-1, DENV-2, and DENV-4) were detected, with DENV-1 dominant in 2021 and 2022, while DENV-2 was dominant in 2020 and 2023. Phylogenetic analyses suggest that the genotypes of DENV-1, DENV-2, and DENV-4 were closely related to corresponding isolates from neighboring countries. These findings provide an update on the nature of DENV cases detected in the Lao PDR and underscore the critical importance of sustaining a robust surveillance network to track infections.https://www.mdpi.com/2076-2607/13/2/318dengueLao PDRserotypeepidemiologyphylogeny
spellingShingle Cécile Troupin
Kedkeo Intavong
Somphavanh Somlor
Souksakhone Viengphouthong
Sitsana Keosenhom
Thep Aksone Chindavong
Phaithong Bounmany
Longthor Vachouaxiong
Thonglakhone Xaybounsou
Chittaphone Vanhnollat
Phonepadith Khattignavong
Darouny Phonekeo
Bouaphanh Khamphaphongphane
Phonepadith Xangsayarath
Vincent Lacoste
Philippe Buchy
Gary Wong
Molecular Epidemiology of Dengue Viruses in Lao People’s Democratic Republic, 2020–2023
Microorganisms
dengue
Lao PDR
serotype
epidemiology
phylogeny
title Molecular Epidemiology of Dengue Viruses in Lao People’s Democratic Republic, 2020–2023
title_full Molecular Epidemiology of Dengue Viruses in Lao People’s Democratic Republic, 2020–2023
title_fullStr Molecular Epidemiology of Dengue Viruses in Lao People’s Democratic Republic, 2020–2023
title_full_unstemmed Molecular Epidemiology of Dengue Viruses in Lao People’s Democratic Republic, 2020–2023
title_short Molecular Epidemiology of Dengue Viruses in Lao People’s Democratic Republic, 2020–2023
title_sort molecular epidemiology of dengue viruses in lao people s democratic republic 2020 2023
topic dengue
Lao PDR
serotype
epidemiology
phylogeny
url https://www.mdpi.com/2076-2607/13/2/318
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