Genome-wide identification and expression analysis of glutaredoxin in Puccinellia tenuiflora under salinity stress
Abstract Background Glutaredoxins (GRX) are key oxidoreductases that modulate protein redox states during plant development and stress responses. Alkaligrass (Puccinellia tenuiflora) is a highly salt-tolerant forage grass, but its GRX gene family (PutGRXs) remains uncharacterized, unlike those in Ar...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-05-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-025-06547-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849311491696623616 |
|---|---|
| author | Yanshuang Liu Xia Han Juanjuan Yu Yueyue Li Meihong Sun Qiuying Pang Ying Li Shaojun Dai |
| author_facet | Yanshuang Liu Xia Han Juanjuan Yu Yueyue Li Meihong Sun Qiuying Pang Ying Li Shaojun Dai |
| author_sort | Yanshuang Liu |
| collection | DOAJ |
| description | Abstract Background Glutaredoxins (GRX) are key oxidoreductases that modulate protein redox states during plant development and stress responses. Alkaligrass (Puccinellia tenuiflora) is a highly salt-tolerant forage grass, but its GRX gene family (PutGRXs) remains uncharacterized, unlike those in Arabidopsis and other plants. Results We identified 25 PutGRX genes in the P. tenuiflora genome. Phylogenetic analysis revealed close evolutionary ties to monocotyledonous rice (Oryza sativa). Based on gene structure and conserved domains, PutGRXs were classified into three groups: five CGFS-type, eleven CPYC-type, and nine CC-type GRXs. Promoter analysis identified numerous cis-acting elements linked to abiotic stresses (e.g., light, drought, heat, cold) and hormone responses, suggesting a pivotal role in stress adaptation. Tissue-specific expression profiling showed differential PutGRX expression in roots, leaves, stems, flowers, and sheaths, with most genes responding to NaCl, NaHCO3, and Na2CO3 stresses. Functional characterization of chloroplast-localized PutGrxS12 demonstrated its importance in plant growth and ROS scavenging under salinity stress. Conclusion This study offers the first comprehensive genomic and functional analysis of the PutGRX family in P. tenuiflora, highlighting its conservation, classification, and stress-responsive roles. Our findings advance understanding of GRX-mediated stress tolerance and provide potential targets for engineering salt-resistant crops. |
| format | Article |
| id | doaj-art-e06450f69b444f4eaaca40234a897163 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-e06450f69b444f4eaaca40234a8971632025-08-20T03:53:22ZengBMCBMC Plant Biology1471-22292025-05-0125111610.1186/s12870-025-06547-1Genome-wide identification and expression analysis of glutaredoxin in Puccinellia tenuiflora under salinity stressYanshuang Liu0Xia Han1Juanjuan Yu2Yueyue Li3Meihong Sun4Qiuying Pang5Ying Li6Shaojun Dai7Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, College of Life Sciences, Northeast Forestry UniversityDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal UniversityCollege of Life Sciences, Henan Normal UniversityKey Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, College of Life Sciences, Northeast Forestry UniversityDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal UniversityKey Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, College of Life Sciences, Northeast Forestry UniversityKey Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, College of Life Sciences, Northeast Forestry UniversityDevelopment Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal UniversityAbstract Background Glutaredoxins (GRX) are key oxidoreductases that modulate protein redox states during plant development and stress responses. Alkaligrass (Puccinellia tenuiflora) is a highly salt-tolerant forage grass, but its GRX gene family (PutGRXs) remains uncharacterized, unlike those in Arabidopsis and other plants. Results We identified 25 PutGRX genes in the P. tenuiflora genome. Phylogenetic analysis revealed close evolutionary ties to monocotyledonous rice (Oryza sativa). Based on gene structure and conserved domains, PutGRXs were classified into three groups: five CGFS-type, eleven CPYC-type, and nine CC-type GRXs. Promoter analysis identified numerous cis-acting elements linked to abiotic stresses (e.g., light, drought, heat, cold) and hormone responses, suggesting a pivotal role in stress adaptation. Tissue-specific expression profiling showed differential PutGRX expression in roots, leaves, stems, flowers, and sheaths, with most genes responding to NaCl, NaHCO3, and Na2CO3 stresses. Functional characterization of chloroplast-localized PutGrxS12 demonstrated its importance in plant growth and ROS scavenging under salinity stress. Conclusion This study offers the first comprehensive genomic and functional analysis of the PutGRX family in P. tenuiflora, highlighting its conservation, classification, and stress-responsive roles. Our findings advance understanding of GRX-mediated stress tolerance and provide potential targets for engineering salt-resistant crops.https://doi.org/10.1186/s12870-025-06547-1Glutaredoxins (GRX) gene familyExpression profilingAbiotic stressPutGrxS12Puccinellia tenuifloraSalt tolerance |
| spellingShingle | Yanshuang Liu Xia Han Juanjuan Yu Yueyue Li Meihong Sun Qiuying Pang Ying Li Shaojun Dai Genome-wide identification and expression analysis of glutaredoxin in Puccinellia tenuiflora under salinity stress BMC Plant Biology Glutaredoxins (GRX) gene family Expression profiling Abiotic stress PutGrxS12 Puccinellia tenuiflora Salt tolerance |
| title | Genome-wide identification and expression analysis of glutaredoxin in Puccinellia tenuiflora under salinity stress |
| title_full | Genome-wide identification and expression analysis of glutaredoxin in Puccinellia tenuiflora under salinity stress |
| title_fullStr | Genome-wide identification and expression analysis of glutaredoxin in Puccinellia tenuiflora under salinity stress |
| title_full_unstemmed | Genome-wide identification and expression analysis of glutaredoxin in Puccinellia tenuiflora under salinity stress |
| title_short | Genome-wide identification and expression analysis of glutaredoxin in Puccinellia tenuiflora under salinity stress |
| title_sort | genome wide identification and expression analysis of glutaredoxin in puccinellia tenuiflora under salinity stress |
| topic | Glutaredoxins (GRX) gene family Expression profiling Abiotic stress PutGrxS12 Puccinellia tenuiflora Salt tolerance |
| url | https://doi.org/10.1186/s12870-025-06547-1 |
| work_keys_str_mv | AT yanshuangliu genomewideidentificationandexpressionanalysisofglutaredoxininpuccinelliatenuifloraundersalinitystress AT xiahan genomewideidentificationandexpressionanalysisofglutaredoxininpuccinelliatenuifloraundersalinitystress AT juanjuanyu genomewideidentificationandexpressionanalysisofglutaredoxininpuccinelliatenuifloraundersalinitystress AT yueyueli genomewideidentificationandexpressionanalysisofglutaredoxininpuccinelliatenuifloraundersalinitystress AT meihongsun genomewideidentificationandexpressionanalysisofglutaredoxininpuccinelliatenuifloraundersalinitystress AT qiuyingpang genomewideidentificationandexpressionanalysisofglutaredoxininpuccinelliatenuifloraundersalinitystress AT yingli genomewideidentificationandexpressionanalysisofglutaredoxininpuccinelliatenuifloraundersalinitystress AT shaojundai genomewideidentificationandexpressionanalysisofglutaredoxininpuccinelliatenuifloraundersalinitystress |