Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats
Objective: This study investigates the effects of GO on depressive behaviors and reproductive parameters in rats exposed to CRS.Materials and Methods: Rats were divided into three groups: sham, CRS-control, and CRS-GO. Behavioral assessments using the SPT and measurements of body and testes weights...
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Mashhad University of Medical Sciences
2025-01-01
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| Series: | Avicenna Journal of Phytomedicine |
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| Online Access: | https://ajp.mums.ac.ir/article_24819_dd7f028d9e32e0172c8d8ac48102a0ab.pdf |
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| author | Mahsan Alizadeh Sina Moshtagh Shahabaddin Abdolalizadeh Amir Mostafa Jeddi Sepehr Tahmasebzadeh Ghazal Radman Amin Bagheri Yasin Bagheri Nazanin Shahabinejad |
| author_facet | Mahsan Alizadeh Sina Moshtagh Shahabaddin Abdolalizadeh Amir Mostafa Jeddi Sepehr Tahmasebzadeh Ghazal Radman Amin Bagheri Yasin Bagheri Nazanin Shahabinejad |
| author_sort | Mahsan Alizadeh |
| collection | DOAJ |
| description | Objective: This study investigates the effects of GO on depressive behaviors and reproductive parameters in rats exposed to CRS.Materials and Methods: Rats were divided into three groups: sham, CRS-control, and CRS-GO. Behavioral assessments using the SPT and measurements of body and testes weights were conducted. Reproductive potential was evaluated by ELISA for testosterone, LH, and FSH levels, as well as sperm characteristics. Oxidative stress levels were assessed through MDA measurements and antioxidant enzyme activities. Additionally, apoptotic and signaling pathway proteins were analyzed by measuring cleaved caspase-3 and Nrf-2 levels in the testes using western blot analysis. Results: GO mitigated depressive behaviors and reduced serum corticosterone levels compared to the CRS-control group. GO increased testosterone, LH, and FSH levels and improved sperm parameters. GO supplementation reduced MDA levels and enhanced antioxidant enzyme activities compared to the CRS-control group. The analysis showed that GO down-regulated cleaved caspase-3 levels and up-regulated Nrf-2 protein levels in the testes of CRS rats compared to the CRS-control group. Conclusion: The administration of GO treatment can contribute to the recovery from male reproductive complications by regulating oxidative stress and apoptotic pathways. |
| format | Article |
| id | doaj-art-e05ba44f61814334ac24058e84f9a5eb |
| institution | Kabale University |
| issn | 2228-7930 2228-7949 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj-art-e05ba44f61814334ac24058e84f9a5eb2025-08-20T03:52:56ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492025-01-0115189090610.22038/ajp.2024.2481924819Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in ratsMahsan Alizadeh0Sina Moshtagh1Shahabaddin Abdolalizadeh Amir2Mostafa Jeddi3Sepehr Tahmasebzadeh4Ghazal Radman5Amin Bagheri6Yasin Bagheri7Nazanin Shahabinejad8Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, IranDepartment of Clinical Science, Theriogenology Section, School of Veterinary Medicine, Razi University, Kermanshah, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, IranDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, IranMolecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, IranDepartment of Urology, Sina Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.Kidney Research Centre, Tabriz University of Medical Sciences, Tabriz, IranStudent Research Committee, Kerman University of Medical Sciences, Kerman, IranObjective: This study investigates the effects of GO on depressive behaviors and reproductive parameters in rats exposed to CRS.Materials and Methods: Rats were divided into three groups: sham, CRS-control, and CRS-GO. Behavioral assessments using the SPT and measurements of body and testes weights were conducted. Reproductive potential was evaluated by ELISA for testosterone, LH, and FSH levels, as well as sperm characteristics. Oxidative stress levels were assessed through MDA measurements and antioxidant enzyme activities. Additionally, apoptotic and signaling pathway proteins were analyzed by measuring cleaved caspase-3 and Nrf-2 levels in the testes using western blot analysis. Results: GO mitigated depressive behaviors and reduced serum corticosterone levels compared to the CRS-control group. GO increased testosterone, LH, and FSH levels and improved sperm parameters. GO supplementation reduced MDA levels and enhanced antioxidant enzyme activities compared to the CRS-control group. The analysis showed that GO down-regulated cleaved caspase-3 levels and up-regulated Nrf-2 protein levels in the testes of CRS rats compared to the CRS-control group. Conclusion: The administration of GO treatment can contribute to the recovery from male reproductive complications by regulating oxidative stress and apoptotic pathways.https://ajp.mums.ac.ir/article_24819_dd7f028d9e32e0172c8d8ac48102a0ab.pdfgamma-oryzanolchronic restraint stresssperm parametersapoptosisoxidative stress |
| spellingShingle | Mahsan Alizadeh Sina Moshtagh Shahabaddin Abdolalizadeh Amir Mostafa Jeddi Sepehr Tahmasebzadeh Ghazal Radman Amin Bagheri Yasin Bagheri Nazanin Shahabinejad Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats Avicenna Journal of Phytomedicine gamma-oryzanol chronic restraint stress sperm parameters apoptosis oxidative stress |
| title | Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats |
| title_full | Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats |
| title_fullStr | Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats |
| title_full_unstemmed | Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats |
| title_short | Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats |
| title_sort | anti oxidant and anti apoptotic effects of gamma oryzanol on male reproductive function in chronic restraint stress in rats |
| topic | gamma-oryzanol chronic restraint stress sperm parameters apoptosis oxidative stress |
| url | https://ajp.mums.ac.ir/article_24819_dd7f028d9e32e0172c8d8ac48102a0ab.pdf |
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