Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats

Anti-oxidant and anti-apoptotic effects of gamma-oryzanol on male reproductive function in chronic restraint stress in rats

Objective: This study investigates the effects of GO on depressive behaviors and reproductive parameters in rats exposed to CRS.Materials and Methods: Rats were divided into three groups: sham, CRS-control, and CRS-GO. Behavioral assessments using the SPT and measurements of body and testes weights...

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Main Authors: Mahsan Alizadeh, Sina Moshtagh, Shahabaddin Abdolalizadeh Amir, Mostafa Jeddi, Sepehr Tahmasebzadeh, Ghazal Radman, Amin Bagheri, Yasin Bagheri, Nazanin Shahabinejad
Format: Article
Language:English
Published: Mashhad University of Medical Sciences 2025-01-01
Series:Avicenna Journal of Phytomedicine
Subjects:
gamma-oryzanol
chronic restraint stress
sperm parameters
apoptosis
oxidative stress
Online Access:https://ajp.mums.ac.ir/article_24819_dd7f028d9e32e0172c8d8ac48102a0ab.pdf
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Summary:Objective: This study investigates the effects of GO on depressive behaviors and reproductive parameters in rats exposed to CRS.Materials and Methods: Rats were divided into three groups: sham, CRS-control, and CRS-GO. Behavioral assessments using the SPT and measurements of body and testes weights were conducted. Reproductive potential was evaluated by ELISA for testosterone, LH, and FSH levels, as well as sperm characteristics. Oxidative stress levels were assessed through MDA measurements and antioxidant enzyme activities. Additionally, apoptotic and signaling pathway proteins were analyzed by measuring cleaved caspase-3 and Nrf-2 levels in the testes using western blot analysis. Results: GO mitigated depressive behaviors and reduced serum corticosterone levels compared to the CRS-control group. GO increased testosterone, LH, and FSH levels and improved sperm parameters. GO supplementation reduced MDA levels and enhanced antioxidant enzyme activities compared to the CRS-control group. The analysis showed that GO down-regulated cleaved caspase-3 levels and up-regulated Nrf-2 protein levels in the testes of CRS rats compared to the CRS-control group. Conclusion: The administration of GO treatment can contribute to the recovery from male reproductive complications by regulating oxidative stress and apoptotic pathways.
ISSN:2228-7930
2228-7949

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