Determination of Phytochemicals and Antimicrobial Activity of Aqueous Stem Bark Extract of Boswellia dalzielii against Some Common Pathogenic Microorganisms
Phytochemical screening by High Performance Liquid Chromatography (HPLC), Fourier Transformed Infrared Spectroscopy (FTIR) and Gas Chromatography- Mass Spectrometry (GCMS) of crude aqueous extract of stem bark of B. dalzielii was performed and its antimicrobial activity on Staphylococcus aureus, St...
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Umaru Musa Yar'adua University, Katsina, Nigeria
2017-06-01
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| Series: | UMYU Journal of Microbiology Research |
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| Online Access: | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/320 |
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| author | Baha’uddeen Salisu Abdulkadir Muhammad Magashi Abdulrazak Mohammed Hussain Usman Adamu |
| author_facet | Baha’uddeen Salisu Abdulkadir Muhammad Magashi Abdulrazak Mohammed Hussain Usman Adamu |
| author_sort | Baha’uddeen Salisu |
| collection | DOAJ |
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Phytochemical screening by High Performance Liquid Chromatography (HPLC), Fourier Transformed Infrared Spectroscopy (FTIR) and Gas Chromatography- Mass Spectrometry (GCMS) of crude aqueous extract of stem bark of B. dalzielii was performed and its antimicrobial activity on Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Salmonella typhi and Candida albicans was evaluated. The result of HPLC analysis revealed 10 components with major ones at peaks 3, 2, 4, 5, 8 and 6 with peak areas of 33.43%, 13.75%, 13.43%, 9.35%, 8.53% and 8.50% respectively; FT-IR revealed 18 functional groups which included amines, amides, a,β–unsaturated aldehydes, ketones, alkanes, alkenes, alkynes, alkyl halides, aromatics, aromatic and aliphatic amines; while the GCMS revealed 9 compounds with major ones as n- Hexadecanoic acid, Stearic acid, 9-Hexadecenoic acid, 1,E-11,Z-13-Octadecatriene with peak areas of 39.40%, 24.28%, 23.38% and 7.73% respectively. The susceptibility test showed that the extract at 50mg/ml to 5mg/ml was active against all the test isolates with higher zones of inhibition of 19±0.00mm for S. typhi and 18±0.30mm for P. mirabilis and 18±0.41mm for P. aeruginosa at 50mg/ml concentration each. Resistance was observed in E. coli and S. pyogenes at 2.5mg/ml concentration. Similarly, lowest MIC values of 12.5mg/ml were obtained for S. typhi and S. aureus and 25mg/ml for C. albicans, P. Mirabilis and P. aeruginosa. K. pneumoniae, E. coli, S. pyogenes and E. faecalis had MIC values of 50mg/ml each. The MBC/MFC values did not exceed the corresponding MIC values by more than a factor of 2. Conclusively, stem bark of B. dazielii contains many antimicrobial active compounds which can be purified and used as precursors for new antibiotics.
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| format | Article |
| id | doaj-art-e04ed33842fa437eb67ea4850703dbf6 |
| institution | OA Journals |
| issn | 2616-0668 2814-1822 |
| language | English |
| publishDate | 2017-06-01 |
| publisher | Umaru Musa Yar'adua University, Katsina, Nigeria |
| record_format | Article |
| series | UMYU Journal of Microbiology Research |
| spelling | doaj-art-e04ed33842fa437eb67ea4850703dbf62025-08-20T02:28:01ZengUmaru Musa Yar'adua University, Katsina, NigeriaUMYU Journal of Microbiology Research2616-06682814-18222017-06-012110.47430/ujmr.1721.035320Determination of Phytochemicals and Antimicrobial Activity of Aqueous Stem Bark Extract of Boswellia dalzielii against Some Common Pathogenic MicroorganismsBaha’uddeen Salisu0Abdulkadir Muhammad Magashi1Abdulrazak Mohammed Hussain2Usman Adamu3Department of Microbiology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Katsina State, Nigeria.Jigawa State Science and Technical Board, Dutse P.M.B. 7087, Jigawa StateJigawa State Science and Technical Board, Dutse P.M.B. 7087, Jigawa StateLaboratory Department, General Hospital Hadejia, Jigawa State, Nigeria. Phytochemical screening by High Performance Liquid Chromatography (HPLC), Fourier Transformed Infrared Spectroscopy (FTIR) and Gas Chromatography- Mass Spectrometry (GCMS) of crude aqueous extract of stem bark of B. dalzielii was performed and its antimicrobial activity on Staphylococcus aureus, Streptococcus pyogenes, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis, Salmonella typhi and Candida albicans was evaluated. The result of HPLC analysis revealed 10 components with major ones at peaks 3, 2, 4, 5, 8 and 6 with peak areas of 33.43%, 13.75%, 13.43%, 9.35%, 8.53% and 8.50% respectively; FT-IR revealed 18 functional groups which included amines, amides, a,β–unsaturated aldehydes, ketones, alkanes, alkenes, alkynes, alkyl halides, aromatics, aromatic and aliphatic amines; while the GCMS revealed 9 compounds with major ones as n- Hexadecanoic acid, Stearic acid, 9-Hexadecenoic acid, 1,E-11,Z-13-Octadecatriene with peak areas of 39.40%, 24.28%, 23.38% and 7.73% respectively. The susceptibility test showed that the extract at 50mg/ml to 5mg/ml was active against all the test isolates with higher zones of inhibition of 19±0.00mm for S. typhi and 18±0.30mm for P. mirabilis and 18±0.41mm for P. aeruginosa at 50mg/ml concentration each. Resistance was observed in E. coli and S. pyogenes at 2.5mg/ml concentration. Similarly, lowest MIC values of 12.5mg/ml were obtained for S. typhi and S. aureus and 25mg/ml for C. albicans, P. Mirabilis and P. aeruginosa. K. pneumoniae, E. coli, S. pyogenes and E. faecalis had MIC values of 50mg/ml each. The MBC/MFC values did not exceed the corresponding MIC values by more than a factor of 2. Conclusively, stem bark of B. dazielii contains many antimicrobial active compounds which can be purified and used as precursors for new antibiotics. https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/320PhytochemicalAntimicrobialAqueousMICBoswellia dalzielii |
| spellingShingle | Baha’uddeen Salisu Abdulkadir Muhammad Magashi Abdulrazak Mohammed Hussain Usman Adamu Determination of Phytochemicals and Antimicrobial Activity of Aqueous Stem Bark Extract of Boswellia dalzielii against Some Common Pathogenic Microorganisms UMYU Journal of Microbiology Research Phytochemical Antimicrobial Aqueous MIC Boswellia dalzielii |
| title | Determination of Phytochemicals and Antimicrobial Activity of Aqueous Stem Bark Extract of Boswellia dalzielii against Some Common Pathogenic Microorganisms |
| title_full | Determination of Phytochemicals and Antimicrobial Activity of Aqueous Stem Bark Extract of Boswellia dalzielii against Some Common Pathogenic Microorganisms |
| title_fullStr | Determination of Phytochemicals and Antimicrobial Activity of Aqueous Stem Bark Extract of Boswellia dalzielii against Some Common Pathogenic Microorganisms |
| title_full_unstemmed | Determination of Phytochemicals and Antimicrobial Activity of Aqueous Stem Bark Extract of Boswellia dalzielii against Some Common Pathogenic Microorganisms |
| title_short | Determination of Phytochemicals and Antimicrobial Activity of Aqueous Stem Bark Extract of Boswellia dalzielii against Some Common Pathogenic Microorganisms |
| title_sort | determination of phytochemicals and antimicrobial activity of aqueous stem bark extract of boswellia dalzielii against some common pathogenic microorganisms |
| topic | Phytochemical Antimicrobial Aqueous MIC Boswellia dalzielii |
| url | https://ujmr.umyu.edu.ng/index.php/ujmr/article/view/320 |
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