Dietary acid load on the Mediterranean and a vegan diet: a secondary analysis of a randomized, cross-over trial

BackgroundEvidence suggests that changes in dietary acid load may influence body weight, and the purpose of this secondary analysis was to assess its role in the context of the Mediterranean and a vegan diet in overweight adults.MethodsIn this randomized cross-over trial, 62 overweight adults were r...

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Main Authors: Hana Kahleova, Cristina Maracine, Joseph Himmelfarb, Arathi Jayaraman, Tatiana Znayenko-Miller, Richard Holubkov, Neal D. Barnard
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Nutrition
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Online Access:https://www.frontiersin.org/articles/10.3389/fnut.2025.1634215/full
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author Hana Kahleova
Cristina Maracine
Joseph Himmelfarb
Arathi Jayaraman
Tatiana Znayenko-Miller
Richard Holubkov
Neal D. Barnard
Neal D. Barnard
author_facet Hana Kahleova
Cristina Maracine
Joseph Himmelfarb
Arathi Jayaraman
Tatiana Znayenko-Miller
Richard Holubkov
Neal D. Barnard
Neal D. Barnard
author_sort Hana Kahleova
collection DOAJ
description BackgroundEvidence suggests that changes in dietary acid load may influence body weight, and the purpose of this secondary analysis was to assess its role in the context of the Mediterranean and a vegan diet in overweight adults.MethodsIn this randomized cross-over trial, 62 overweight adults were randomized to a Mediterranean or a low-fat vegan diet for 16-weeks, separated by a 4-week washout. Change in body weight was the primary outcome. Three-day dietary records were analyzed, and Potential Renal Acid Load (PRAL) and Net Endogenous Acid Production (NEAP) were calculated as markers of dietary acid load, and their relationship was tested with changes in body weight.ResultsCompared with no change on the Mediterranean diet, PRAL and NEAP significantly decreased on the vegan diet; effect sizes: −25.8 (95% CI −34.1 to −17.5); p < 0.001; and −27.1 (95% CI −35.4 to −18.7); p < 0.001, respectively. Across both diets, changes in PRAL and NEAP were positively associated with changes in body weight in the first 16 weeks of the study: r = +0.34; p = 0.009; and r = +0.39; p = 0.002, respectively, as well as in the second 16 weeks: r = +0.59; p < 0.001, and r = +0.61; p < 0.001, respectively.ConclusionThese findings suggest that, compared with the Mediterranean diet, dietary acid load decreased significantly on the low-fat vegan diet and was associated with weight loss. The alkalizing effect of a vegan diet may be an independent mechanism by which a vegan diet promotes weight loss.Clinical trial registrationhttps://clinicaltrials.gov/, identifier NCT03698955.
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spelling doaj-art-e04c60c93a1047c1aed92f386f6c88132025-08-20T03:32:12ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-06-011210.3389/fnut.2025.16342151634215Dietary acid load on the Mediterranean and a vegan diet: a secondary analysis of a randomized, cross-over trialHana Kahleova0Cristina Maracine1Joseph Himmelfarb2Arathi Jayaraman3Tatiana Znayenko-Miller4Richard Holubkov5Neal D. Barnard6Neal D. Barnard7Physicians Committee for Responsible Medicine, Washington, DC, United StatesPhysicians Committee for Responsible Medicine, Washington, DC, United StatesPhysicians Committee for Responsible Medicine, Washington, DC, United StatesPhysicians Committee for Responsible Medicine, Washington, DC, United StatesPhysicians Committee for Responsible Medicine, Washington, DC, United StatesSchool of Medicine, University of Utah, Salt Lake City, UT, United StatesPhysicians Committee for Responsible Medicine, Washington, DC, United StatesGeorge Washington University School of Medicine and Health Sciences, Washington, DC, United StatesBackgroundEvidence suggests that changes in dietary acid load may influence body weight, and the purpose of this secondary analysis was to assess its role in the context of the Mediterranean and a vegan diet in overweight adults.MethodsIn this randomized cross-over trial, 62 overweight adults were randomized to a Mediterranean or a low-fat vegan diet for 16-weeks, separated by a 4-week washout. Change in body weight was the primary outcome. Three-day dietary records were analyzed, and Potential Renal Acid Load (PRAL) and Net Endogenous Acid Production (NEAP) were calculated as markers of dietary acid load, and their relationship was tested with changes in body weight.ResultsCompared with no change on the Mediterranean diet, PRAL and NEAP significantly decreased on the vegan diet; effect sizes: −25.8 (95% CI −34.1 to −17.5); p < 0.001; and −27.1 (95% CI −35.4 to −18.7); p < 0.001, respectively. Across both diets, changes in PRAL and NEAP were positively associated with changes in body weight in the first 16 weeks of the study: r = +0.34; p = 0.009; and r = +0.39; p = 0.002, respectively, as well as in the second 16 weeks: r = +0.59; p < 0.001, and r = +0.61; p < 0.001, respectively.ConclusionThese findings suggest that, compared with the Mediterranean diet, dietary acid load decreased significantly on the low-fat vegan diet and was associated with weight loss. The alkalizing effect of a vegan diet may be an independent mechanism by which a vegan diet promotes weight loss.Clinical trial registrationhttps://clinicaltrials.gov/, identifier NCT03698955.https://www.frontiersin.org/articles/10.3389/fnut.2025.1634215/fulldietary acid loadMediterraneannutritionplant-basedvegan
spellingShingle Hana Kahleova
Cristina Maracine
Joseph Himmelfarb
Arathi Jayaraman
Tatiana Znayenko-Miller
Richard Holubkov
Neal D. Barnard
Neal D. Barnard
Dietary acid load on the Mediterranean and a vegan diet: a secondary analysis of a randomized, cross-over trial
Frontiers in Nutrition
dietary acid load
Mediterranean
nutrition
plant-based
vegan
title Dietary acid load on the Mediterranean and a vegan diet: a secondary analysis of a randomized, cross-over trial
title_full Dietary acid load on the Mediterranean and a vegan diet: a secondary analysis of a randomized, cross-over trial
title_fullStr Dietary acid load on the Mediterranean and a vegan diet: a secondary analysis of a randomized, cross-over trial
title_full_unstemmed Dietary acid load on the Mediterranean and a vegan diet: a secondary analysis of a randomized, cross-over trial
title_short Dietary acid load on the Mediterranean and a vegan diet: a secondary analysis of a randomized, cross-over trial
title_sort dietary acid load on the mediterranean and a vegan diet a secondary analysis of a randomized cross over trial
topic dietary acid load
Mediterranean
nutrition
plant-based
vegan
url https://www.frontiersin.org/articles/10.3389/fnut.2025.1634215/full
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