SPECIFIC DETECTION OF Salmonella spp. WITH MOLECULAR BIOLOGICAL TECHNIQUES
A method for specific detection of Salmonella spp. was developed based on 16S rRNA targeted PCR (polymerase chain reaction). A method based on recognition of specific region of 16S rRNA gene was published previously by Lin and Tsen in 1996. The sequence analysis of recently determined small subunit...
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| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
1998-12-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/17091 |
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| author | Marija TRKOV G. AVGUŠTIN |
| author_facet | Marija TRKOV G. AVGUŠTIN |
| author_sort | Marija TRKOV |
| collection | DOAJ |
| description | A method for specific detection of Salmonella spp. was developed based on 16S rRNA targeted PCR (polymerase chain reaction). A method based on recognition of specific region of 16S rRNA gene was published previously by Lin and Tsen in 1996. The sequence analysis of recently determined small subunit ribosomal genes from Salmonella spp. showed however, that the 16SF1 and 16SIII primers are not suitable for specific amplification of all Salmonella strains. 16SF1 was modified and a new forward PCR primer was designed. The specificity of the method was tested using 87 Salmonella strains and 30 non-Salmonella strains of the family Enterobacteriaceae and compared with two other previously published methods and found to be 100% specific. The reverse primer was also labelled with a tetramethylrhodamine isothyiocianate fluorescent dye and successfully used as a probe in in situ experiments. |
| format | Article |
| id | doaj-art-e045a3745af9429798da5d047b97726e |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 1998-12-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-e045a3745af9429798da5d047b97726e2025-08-20T03:02:26ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19411998-12-0172112312710.14720/aas.1998.72.1.1709123485SPECIFIC DETECTION OF Salmonella spp. WITH MOLECULAR BIOLOGICAL TECHNIQUESMarija TRKOV0G. AVGUŠTIN1Univ. of Ljubljana, Biotechnical Fac., Dept. Food Sci. Tech., Ljubljana, SloveniaUniv. of Ljubljana, Biotechnical Fac., Zootechnical Dept., Domžale, SloveniaA method for specific detection of Salmonella spp. was developed based on 16S rRNA targeted PCR (polymerase chain reaction). A method based on recognition of specific region of 16S rRNA gene was published previously by Lin and Tsen in 1996. The sequence analysis of recently determined small subunit ribosomal genes from Salmonella spp. showed however, that the 16SF1 and 16SIII primers are not suitable for specific amplification of all Salmonella strains. 16SF1 was modified and a new forward PCR primer was designed. The specificity of the method was tested using 87 Salmonella strains and 30 non-Salmonella strains of the family Enterobacteriaceae and compared with two other previously published methods and found to be 100% specific. The reverse primer was also labelled with a tetramethylrhodamine isothyiocianate fluorescent dye and successfully used as a probe in in situ experiments.https://journals.uni-lj.si/aas/article/view/17091microbiologybacteriasalmonellamolecular biologymethodsmolecular geneticrrna |
| spellingShingle | Marija TRKOV G. AVGUŠTIN SPECIFIC DETECTION OF Salmonella spp. WITH MOLECULAR BIOLOGICAL TECHNIQUES Acta Agriculturae Slovenica microbiology bacteria salmonella molecular biology methods molecular genetic rrna |
| title | SPECIFIC DETECTION OF Salmonella spp. WITH MOLECULAR BIOLOGICAL TECHNIQUES |
| title_full | SPECIFIC DETECTION OF Salmonella spp. WITH MOLECULAR BIOLOGICAL TECHNIQUES |
| title_fullStr | SPECIFIC DETECTION OF Salmonella spp. WITH MOLECULAR BIOLOGICAL TECHNIQUES |
| title_full_unstemmed | SPECIFIC DETECTION OF Salmonella spp. WITH MOLECULAR BIOLOGICAL TECHNIQUES |
| title_short | SPECIFIC DETECTION OF Salmonella spp. WITH MOLECULAR BIOLOGICAL TECHNIQUES |
| title_sort | specific detection of salmonella spp with molecular biological techniques |
| topic | microbiology bacteria salmonella molecular biology methods molecular genetic rrna |
| url | https://journals.uni-lj.si/aas/article/view/17091 |
| work_keys_str_mv | AT marijatrkov specificdetectionofsalmonellasppwithmolecularbiologicaltechniques AT gavgustin specificdetectionofsalmonellasppwithmolecularbiologicaltechniques |