An immunoinformatic investigation on Rift Valley fever virus protein reveals possible epitopes for vaccines
Introduction: This immunoinformatic study identified potential epitopes from the envelopment polyprotein (Gn/Gc) of Rift Valley fever virus (RVFV), a pathogenic virus causing severe fever in humans and livestock. Effective vaccination is crucial for controlling RVFV outbreaks. The identification of...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2024-07-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/19005 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849313843600162816 |
|---|---|
| author | Tanjir Hosen Saaimatul Huq Mohammad Abdullah-Al-Shoeb Shahidul Islam Muhammad Abul Kalam Azad |
| author_facet | Tanjir Hosen Saaimatul Huq Mohammad Abdullah-Al-Shoeb Shahidul Islam Muhammad Abul Kalam Azad |
| author_sort | Tanjir Hosen |
| collection | DOAJ |
| description |
Introduction: This immunoinformatic study identified potential epitopes from the envelopment polyprotein (Gn/Gc) of Rift Valley fever virus (RVFV), a pathogenic virus causing severe fever in humans and livestock. Effective vaccination is crucial for controlling RVFV outbreaks. The identification of suitable epitopes is crucial for the development of safe and effective vaccines.
Methodology: Protein sequences were obtained from the UniProt database, and evaluated through VaxiJen v2.0 to predict the B and T-cell epitopes within the RVFV glycoprotein. Gn/Gc protein sequences were analyzed with bioinformatics tools and algorithms. The predicted T-cell and B-cell epitopes were evaluated for antigenicity, allergenicity, and toxicity by the VaxiJen v2.0 system, AllerTop v2.0, and ToxinPred server, respectively.
Results: We employed computational methods to screen the RVFV envelopment polyprotein encompassing N-terminal and C-terminal glycoprotein segments, to discover antigenic T- and B-cell epitopes. Our analysis unveiled multiple potential epitopes within the RVFV glycoprotein, specifically within the Gn/Gc protein sequences. Subsequently, we selected eleven cytotoxic T-lymphocytes (CTL) and four helper T-lymphocytes (HTL) for population coverage analysis, which collectively extended to cover 97.04% of the world's population, representing diverse ethnicities and regions. Notably, the CTL epitope VQADLTLMF exhibited binding affinity to numerous human leukocyte antigen (HLA) alleles. The identification of glycoprotein (Gn/Gc) epitopes through this immunoinformatic study bears significant implications for advancing the development of an effective RVFV vaccine.
Conclusions: These findings provide valuable insights into the immunological aspects of the disease and may contribute towards the development of broad-spectrum antiviral therapies targeting other RNA viruses with similar polymerase enzymes.
|
| format | Article |
| id | doaj-art-e04500a886ec4f5f920b0a5247d6c12b |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-e04500a886ec4f5f920b0a5247d6c12b2025-08-20T03:52:38ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-07-01180710.3855/jidc.19005An immunoinformatic investigation on Rift Valley fever virus protein reveals possible epitopes for vaccinesTanjir Hosen0Saaimatul Huq1Mohammad Abdullah-Al-Shoeb2Shahidul Islam3Muhammad Abul Kalam Azad4Department of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet 3114, BangladeshDepartment of Radiation Oncology, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR 72205, United StatesDepartment of Biochemistry and Molecular Biology, Shahjalal University of Science and Technology, Sylhet 3114, BangladeshDepartment of Agriculture/Agricultural Regulations, 1200 N. University Drive, University of Arkansas at Pine Bluff, AR 71601, United StatesDepartment of Agriculture/Agricultural Regulations, 1200 N. University Drive, University of Arkansas at Pine Bluff, AR 71601, United States Introduction: This immunoinformatic study identified potential epitopes from the envelopment polyprotein (Gn/Gc) of Rift Valley fever virus (RVFV), a pathogenic virus causing severe fever in humans and livestock. Effective vaccination is crucial for controlling RVFV outbreaks. The identification of suitable epitopes is crucial for the development of safe and effective vaccines. Methodology: Protein sequences were obtained from the UniProt database, and evaluated through VaxiJen v2.0 to predict the B and T-cell epitopes within the RVFV glycoprotein. Gn/Gc protein sequences were analyzed with bioinformatics tools and algorithms. The predicted T-cell and B-cell epitopes were evaluated for antigenicity, allergenicity, and toxicity by the VaxiJen v2.0 system, AllerTop v2.0, and ToxinPred server, respectively. Results: We employed computational methods to screen the RVFV envelopment polyprotein encompassing N-terminal and C-terminal glycoprotein segments, to discover antigenic T- and B-cell epitopes. Our analysis unveiled multiple potential epitopes within the RVFV glycoprotein, specifically within the Gn/Gc protein sequences. Subsequently, we selected eleven cytotoxic T-lymphocytes (CTL) and four helper T-lymphocytes (HTL) for population coverage analysis, which collectively extended to cover 97.04% of the world's population, representing diverse ethnicities and regions. Notably, the CTL epitope VQADLTLMF exhibited binding affinity to numerous human leukocyte antigen (HLA) alleles. The identification of glycoprotein (Gn/Gc) epitopes through this immunoinformatic study bears significant implications for advancing the development of an effective RVFV vaccine. Conclusions: These findings provide valuable insights into the immunological aspects of the disease and may contribute towards the development of broad-spectrum antiviral therapies targeting other RNA viruses with similar polymerase enzymes. https://jidc.org/index.php/journal/article/view/19005immunoinformaticRVFVglycoproteinGn/GcB-cell epitopesT-cell epitopes |
| spellingShingle | Tanjir Hosen Saaimatul Huq Mohammad Abdullah-Al-Shoeb Shahidul Islam Muhammad Abul Kalam Azad An immunoinformatic investigation on Rift Valley fever virus protein reveals possible epitopes for vaccines Journal of Infection in Developing Countries immunoinformatic RVFV glycoprotein Gn/Gc B-cell epitopes T-cell epitopes |
| title | An immunoinformatic investigation on Rift Valley fever virus protein reveals possible epitopes for vaccines |
| title_full | An immunoinformatic investigation on Rift Valley fever virus protein reveals possible epitopes for vaccines |
| title_fullStr | An immunoinformatic investigation on Rift Valley fever virus protein reveals possible epitopes for vaccines |
| title_full_unstemmed | An immunoinformatic investigation on Rift Valley fever virus protein reveals possible epitopes for vaccines |
| title_short | An immunoinformatic investigation on Rift Valley fever virus protein reveals possible epitopes for vaccines |
| title_sort | immunoinformatic investigation on rift valley fever virus protein reveals possible epitopes for vaccines |
| topic | immunoinformatic RVFV glycoprotein Gn/Gc B-cell epitopes T-cell epitopes |
| url | https://jidc.org/index.php/journal/article/view/19005 |
| work_keys_str_mv | AT tanjirhosen animmunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines AT saaimatulhuq animmunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines AT mohammadabdullahalshoeb animmunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines AT shahidulislam animmunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines AT muhammadabulkalamazad animmunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines AT tanjirhosen immunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines AT saaimatulhuq immunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines AT mohammadabdullahalshoeb immunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines AT shahidulislam immunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines AT muhammadabulkalamazad immunoinformaticinvestigationonriftvalleyfevervirusproteinrevealspossibleepitopesforvaccines |