miRNAs-Based Molecular Signature for KRAS Mutated and Wild Type Colorectal Cancer: An Explorative Study
microRNAs (miRNAs) have been proposed as promising molecular biomarkers for diagnosis, prognosis, and responsive therapeutic targets in different types of cancer, including colorectal cancer (CRC). In this study, we evaluated the expression levels of 84 cancer-associated miRNAs in a cohort of 39 hum...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/4927120 |
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| author | Elena Milanesi Maria Dobre Alina Ioana Bucuroiu Vlad Herlea Teodora Ecaterina Manuc Alessandro Salvi Giuseppina De Petro Mircea Manuc Gabriel Becheanu |
| author_facet | Elena Milanesi Maria Dobre Alina Ioana Bucuroiu Vlad Herlea Teodora Ecaterina Manuc Alessandro Salvi Giuseppina De Petro Mircea Manuc Gabriel Becheanu |
| author_sort | Elena Milanesi |
| collection | DOAJ |
| description | microRNAs (miRNAs) have been proposed as promising molecular biomarkers for diagnosis, prognosis, and responsive therapeutic targets in different types of cancer, including colorectal cancer (CRC). In this study, we evaluated the expression levels of 84 cancer-associated miRNAs in a cohort of 39 human samples comprising 13 peritumoral and 26 tumoral tissues from surgical specimens of CRC patients. KRAS mutations were detected in 11 tumoral samples. In a first analysis, we found 5 miRNAs (miR-215-5p, miR-9-5p, miR-138-5p, miR378a-3p, and miR-150-5p) that were significantly downregulated and one upregulated (miR-135b-5p) in tumoral tissues compared with the peritumoral tissues. Furthermore, by comparing miRNA profile between KRAS mutated CRC tissues respect to wild type CRC tissues, we found 7 miRNA (miR-27b-3p, miR-191-5p, miR-let7d-5p, miR-15b-5p, miR-98-5p, miR-10a-5p, and miR-149-5p) downregulated in KRAS mutated condition. In conclusion, we have identified a panel of miRNAs that specifically distinguish CRC tissues from peritumoral tissue and a different set of miRNAs specific for CRC with KRAS mutations. These findings may contribute to the discovering of new molecular biomarkers with clinic relevance and might shed light on novel molecular aspects of CRC. |
| format | Article |
| id | doaj-art-e041dffbcb1e4018a0d01f6584d984bf |
| institution | DOAJ |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e041dffbcb1e4018a0d01f6584d984bf2025-08-20T03:20:57ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/49271204927120miRNAs-Based Molecular Signature for KRAS Mutated and Wild Type Colorectal Cancer: An Explorative StudyElena Milanesi0Maria Dobre1Alina Ioana Bucuroiu2Vlad Herlea3Teodora Ecaterina Manuc4Alessandro Salvi5Giuseppina De Petro6Mircea Manuc7Gabriel Becheanu8Victor Babes National Institute of Pathology, 050096 Bucharest, RomaniaVictor Babes National Institute of Pathology, 050096 Bucharest, RomaniaCarol Davila University of Medicine and Pharmacy, 050474 Bucharest, RomaniaFundeni Clinical Institute, 022328 Bucharest, RomaniaCarol Davila University of Medicine and Pharmacy, 050474 Bucharest, RomaniaDivision of Biology and Genetics, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, ItalyDivision of Biology and Genetics, Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, ItalyCarol Davila University of Medicine and Pharmacy, 050474 Bucharest, RomaniaVictor Babes National Institute of Pathology, 050096 Bucharest, RomaniamicroRNAs (miRNAs) have been proposed as promising molecular biomarkers for diagnosis, prognosis, and responsive therapeutic targets in different types of cancer, including colorectal cancer (CRC). In this study, we evaluated the expression levels of 84 cancer-associated miRNAs in a cohort of 39 human samples comprising 13 peritumoral and 26 tumoral tissues from surgical specimens of CRC patients. KRAS mutations were detected in 11 tumoral samples. In a first analysis, we found 5 miRNAs (miR-215-5p, miR-9-5p, miR-138-5p, miR378a-3p, and miR-150-5p) that were significantly downregulated and one upregulated (miR-135b-5p) in tumoral tissues compared with the peritumoral tissues. Furthermore, by comparing miRNA profile between KRAS mutated CRC tissues respect to wild type CRC tissues, we found 7 miRNA (miR-27b-3p, miR-191-5p, miR-let7d-5p, miR-15b-5p, miR-98-5p, miR-10a-5p, and miR-149-5p) downregulated in KRAS mutated condition. In conclusion, we have identified a panel of miRNAs that specifically distinguish CRC tissues from peritumoral tissue and a different set of miRNAs specific for CRC with KRAS mutations. These findings may contribute to the discovering of new molecular biomarkers with clinic relevance and might shed light on novel molecular aspects of CRC.http://dx.doi.org/10.1155/2020/4927120 |
| spellingShingle | Elena Milanesi Maria Dobre Alina Ioana Bucuroiu Vlad Herlea Teodora Ecaterina Manuc Alessandro Salvi Giuseppina De Petro Mircea Manuc Gabriel Becheanu miRNAs-Based Molecular Signature for KRAS Mutated and Wild Type Colorectal Cancer: An Explorative Study Journal of Immunology Research |
| title | miRNAs-Based Molecular Signature for KRAS Mutated and Wild Type Colorectal Cancer: An Explorative Study |
| title_full | miRNAs-Based Molecular Signature for KRAS Mutated and Wild Type Colorectal Cancer: An Explorative Study |
| title_fullStr | miRNAs-Based Molecular Signature for KRAS Mutated and Wild Type Colorectal Cancer: An Explorative Study |
| title_full_unstemmed | miRNAs-Based Molecular Signature for KRAS Mutated and Wild Type Colorectal Cancer: An Explorative Study |
| title_short | miRNAs-Based Molecular Signature for KRAS Mutated and Wild Type Colorectal Cancer: An Explorative Study |
| title_sort | mirnas based molecular signature for kras mutated and wild type colorectal cancer an explorative study |
| url | http://dx.doi.org/10.1155/2020/4927120 |
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