Compound Heterozygous MRPS14 Variants Associated With Leigh Syndrome

ABSTRACT MRPS14 (uS14m) is a nuclear‐encoded ribosomal protein important for mitochondria‐specific translation. To date, only a single individual with a recessive MRPS14‐related disorder (also known as COXPD38) has been reported. We report an additional subject possessing novel compound heterozygous...

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Main Authors: Maria Gabriela Otero, Christina Freeman, Ruchi Shah, Renkui Bai, Hong Cui, Marian Castro, Zachary Myers, Eric Choy, Derek Chan, Molly Easter, Sophia Y. Zhao, Madeline Babros, Ruchi Garg, Matthew Deardorff, Franklin Moser, Tyler Mark Pierson
Format: Article
Language:English
Published: Wiley 2025-08-01
Series:Annals of Clinical and Translational Neurology
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Online Access:https://doi.org/10.1002/acn3.70065
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author Maria Gabriela Otero
Christina Freeman
Ruchi Shah
Renkui Bai
Hong Cui
Marian Castro
Zachary Myers
Eric Choy
Derek Chan
Molly Easter
Sophia Y. Zhao
Madeline Babros
Ruchi Garg
Matthew Deardorff
Franklin Moser
Tyler Mark Pierson
author_facet Maria Gabriela Otero
Christina Freeman
Ruchi Shah
Renkui Bai
Hong Cui
Marian Castro
Zachary Myers
Eric Choy
Derek Chan
Molly Easter
Sophia Y. Zhao
Madeline Babros
Ruchi Garg
Matthew Deardorff
Franklin Moser
Tyler Mark Pierson
author_sort Maria Gabriela Otero
collection DOAJ
description ABSTRACT MRPS14 (uS14m) is a nuclear‐encoded ribosomal protein important for mitochondria‐specific translation. To date, only a single individual with a recessive MRPS14‐related disorder (also known as COXPD38) has been reported. We report an additional subject possessing novel compound heterozygous MRPS14 variants (p.Asp37Asn, p.Asn60Asp). The subject presented at 2 years with motor and language delays associated with elevated serum lactate/alanine levels. Brain MRI showed a constellation of signal abnormalities consistent with Leigh Syndrome, while MR spectroscopy had an increased lactate peak. Western blots of fibroblasts showed decreased MRPS14 and COX2 protein levels. These results support the pathogenicity of the MRPS14 variants identified here.
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issn 2328-9503
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series Annals of Clinical and Translational Neurology
spelling doaj-art-e0410a8f12104a0090e2d00e63576e412025-08-20T03:36:49ZengWileyAnnals of Clinical and Translational Neurology2328-95032025-08-011281693169710.1002/acn3.70065Compound Heterozygous MRPS14 Variants Associated With Leigh SyndromeMaria Gabriela Otero0Christina Freeman1Ruchi Shah2Renkui Bai3Hong Cui4Marian Castro5Zachary Myers6Eric Choy7Derek Chan8Molly Easter9Sophia Y. Zhao10Madeline Babros11Ruchi Garg12Matthew Deardorff13Franklin Moser14Tyler Mark Pierson15Board of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USABoard of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USABiomedical Sciences Cedars‐Sinai Medical Center Los Angeles California USAGeneDx Gaithersburg Maryland USAGeneDx Gaithersburg Maryland USABoard of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USABoard of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USABoard of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USABoard of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USABoard of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USABoard of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USABoard of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USAPediatric Congenital Heart Program at Cedars‐Sinai Guerin Children's and Smidt Heart Institute Cedars‐Sinai Medical Center Los Angeles California USADepartment of Pathology and Laboratory Medicine Children's Hospital Los Angeles California Los Angeles USADivision of Clinical Neuroradiology and Interventional Neuroradiology Cedars‐Sinai Medical Center Los Angeles California USABoard of Governors Regenerative Medicine Institute Cedars‐Sinai Medical Center Los Angeles California USAABSTRACT MRPS14 (uS14m) is a nuclear‐encoded ribosomal protein important for mitochondria‐specific translation. To date, only a single individual with a recessive MRPS14‐related disorder (also known as COXPD38) has been reported. We report an additional subject possessing novel compound heterozygous MRPS14 variants (p.Asp37Asn, p.Asn60Asp). The subject presented at 2 years with motor and language delays associated with elevated serum lactate/alanine levels. Brain MRI showed a constellation of signal abnormalities consistent with Leigh Syndrome, while MR spectroscopy had an increased lactate peak. Western blots of fibroblasts showed decreased MRPS14 and COX2 protein levels. These results support the pathogenicity of the MRPS14 variants identified here.https://doi.org/10.1002/acn3.70065Leigh Syndromemitochondrial ribosomeMRPS14oxidative phosphorylation
spellingShingle Maria Gabriela Otero
Christina Freeman
Ruchi Shah
Renkui Bai
Hong Cui
Marian Castro
Zachary Myers
Eric Choy
Derek Chan
Molly Easter
Sophia Y. Zhao
Madeline Babros
Ruchi Garg
Matthew Deardorff
Franklin Moser
Tyler Mark Pierson
Compound Heterozygous MRPS14 Variants Associated With Leigh Syndrome
Annals of Clinical and Translational Neurology
Leigh Syndrome
mitochondrial ribosome
MRPS14
oxidative phosphorylation
title Compound Heterozygous MRPS14 Variants Associated With Leigh Syndrome
title_full Compound Heterozygous MRPS14 Variants Associated With Leigh Syndrome
title_fullStr Compound Heterozygous MRPS14 Variants Associated With Leigh Syndrome
title_full_unstemmed Compound Heterozygous MRPS14 Variants Associated With Leigh Syndrome
title_short Compound Heterozygous MRPS14 Variants Associated With Leigh Syndrome
title_sort compound heterozygous mrps14 variants associated with leigh syndrome
topic Leigh Syndrome
mitochondrial ribosome
MRPS14
oxidative phosphorylation
url https://doi.org/10.1002/acn3.70065
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