Effect of processing methods on fatty acid composition and flavour profile of clarified butter (ghee) obtained from Deoni and Holstein Friesian cow breeds

The study investigated the impact of three processes—curd-butter (CD), cream-butter (CM) and fermented cream-butter (FC) on fatty acid composition and volatile profiles of clarified butter (ghee) obtained from two cow breeds, Deoni (DN) and Holstein Friesian (HF). Gas chromatography-mass spectrometr...

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Main Authors: Deepshikha Kataria, Gurmeet Singh
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157525003360
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author Deepshikha Kataria
Gurmeet Singh
author_facet Deepshikha Kataria
Gurmeet Singh
author_sort Deepshikha Kataria
collection DOAJ
description The study investigated the impact of three processes—curd-butter (CD), cream-butter (CM) and fermented cream-butter (FC) on fatty acid composition and volatile profiles of clarified butter (ghee) obtained from two cow breeds, Deoni (DN) and Holstein Friesian (HF). Gas chromatography-mass spectrometry (GC–MS) identified 26 fatty acids in ghee. There was no difference in fatty acid composition due to processing. Significant differences were observed by breed, with HF ghee exhibiting higher polyunsaturated fatty acids and α-linolenic acid than DN ghee. Headspace GC–MS detected 57 volatile compounds. Ghee made with CD and FC processes exhibited higher levels of acids, alcohols, heat degradation products, lactones, ketones, 5-hydroxymethylfurfural and maltol in both breeds. These findings suggest that processing methods alter ghee's volatile compounds without significantly affecting fatty acid composition. Since ghee is a complex lipid, untargeted lipidomics and metabolomics studies can provide further insight into the effects of processing on ghee. This study can guide dairy processors in selecting milk sources and ghee processes to enhance flavour in ghee while maintaining fatty acid composition across cow breeds for product improvement.
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spelling doaj-art-e03c8f6530ff475688241d61c7dab9dd2025-08-20T02:31:51ZengElsevierFood Chemistry: X2590-15752025-04-012710248910.1016/j.fochx.2025.102489Effect of processing methods on fatty acid composition and flavour profile of clarified butter (ghee) obtained from Deoni and Holstein Friesian cow breedsDeepshikha Kataria0Gurmeet Singh1The University of Trans-Disciplinary Health Sciences and Technology, Bengaluru 560064, IndiaCorresponding author at: The University of Trans-Disciplinary Health Sciences and Technology, 74/2, Jarakabande Kaval, Post Attur via Yelahanka, Bengaluru 560064, India.; The University of Trans-Disciplinary Health Sciences and Technology, Bengaluru 560064, IndiaThe study investigated the impact of three processes—curd-butter (CD), cream-butter (CM) and fermented cream-butter (FC) on fatty acid composition and volatile profiles of clarified butter (ghee) obtained from two cow breeds, Deoni (DN) and Holstein Friesian (HF). Gas chromatography-mass spectrometry (GC–MS) identified 26 fatty acids in ghee. There was no difference in fatty acid composition due to processing. Significant differences were observed by breed, with HF ghee exhibiting higher polyunsaturated fatty acids and α-linolenic acid than DN ghee. Headspace GC–MS detected 57 volatile compounds. Ghee made with CD and FC processes exhibited higher levels of acids, alcohols, heat degradation products, lactones, ketones, 5-hydroxymethylfurfural and maltol in both breeds. These findings suggest that processing methods alter ghee's volatile compounds without significantly affecting fatty acid composition. Since ghee is a complex lipid, untargeted lipidomics and metabolomics studies can provide further insight into the effects of processing on ghee. This study can guide dairy processors in selecting milk sources and ghee processes to enhance flavour in ghee while maintaining fatty acid composition across cow breeds for product improvement.http://www.sciencedirect.com/science/article/pii/S2590157525003360Clarified butterFatty acidVolatile compoundsDeoniHolstein Friesian
spellingShingle Deepshikha Kataria
Gurmeet Singh
Effect of processing methods on fatty acid composition and flavour profile of clarified butter (ghee) obtained from Deoni and Holstein Friesian cow breeds
Food Chemistry: X
Clarified butter
Fatty acid
Volatile compounds
Deoni
Holstein Friesian
title Effect of processing methods on fatty acid composition and flavour profile of clarified butter (ghee) obtained from Deoni and Holstein Friesian cow breeds
title_full Effect of processing methods on fatty acid composition and flavour profile of clarified butter (ghee) obtained from Deoni and Holstein Friesian cow breeds
title_fullStr Effect of processing methods on fatty acid composition and flavour profile of clarified butter (ghee) obtained from Deoni and Holstein Friesian cow breeds
title_full_unstemmed Effect of processing methods on fatty acid composition and flavour profile of clarified butter (ghee) obtained from Deoni and Holstein Friesian cow breeds
title_short Effect of processing methods on fatty acid composition and flavour profile of clarified butter (ghee) obtained from Deoni and Holstein Friesian cow breeds
title_sort effect of processing methods on fatty acid composition and flavour profile of clarified butter ghee obtained from deoni and holstein friesian cow breeds
topic Clarified butter
Fatty acid
Volatile compounds
Deoni
Holstein Friesian
url http://www.sciencedirect.com/science/article/pii/S2590157525003360
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