Integration of bioaccumulations, chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic, cadmium and lead in the brown seaweed Sargassum fusiforme
This study investigated the accumulation and detoxification mechanisms of inorganic arsenic (iAs), cadmium (Cd), and lead (Pb) in Sargassum fusiforme through controlled laboratory exposure experiments combined with chemical speciation analysis and comparative transcriptomics. Our results revealed th...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325007936 |
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| author | Yanfang Zhao Yingying Guo Jifa Wu Xuming Kang Xiaofeng Sheng Zhijun Tan |
| author_facet | Yanfang Zhao Yingying Guo Jifa Wu Xuming Kang Xiaofeng Sheng Zhijun Tan |
| author_sort | Yanfang Zhao |
| collection | DOAJ |
| description | This study investigated the accumulation and detoxification mechanisms of inorganic arsenic (iAs), cadmium (Cd), and lead (Pb) in Sargassum fusiforme through controlled laboratory exposure experiments combined with chemical speciation analysis and comparative transcriptomics. Our results revealed that S. fusiforme exhibited similar accumulation capacities for arsenite [As(III)] and arsenate [As(V)], with the less toxic As(V) being the predominant species in both treatment groups. The algae demonstrated higher accumulation capacity for Pb compared to Cd. Chemical speciation analysis indicated that S. fusiforme primarily detoxified Cd through organic ligand binding, while Pb was predominantly immobilized as insoluble compounds. Transcriptomic analysis revealed metal-specific detoxification pathways: As(III) exposure significantly enriched pathways related to free radical scavenging, including phenylpropanoid biosynthesis, stilbenoid/diarylheptanoid/gingerol biosynthesis, and flavonoid biosynthesis; As(V) treatment notably affected oxidative phosphorylation among five significantly enriched pathways; Cd exposure triggered complex responses involving 23 significantly enriched pathways; Pb treatment primarily influenced ribosomal biogenesis and translation among four enriched pathways. These findings demonstrated distinct detoxification mechanisms in S. fusiforme for each of the four studied toxic elements. |
| format | Article |
| id | doaj-art-e03a4af84a13490aad1bd54aa12e2f26 |
| institution | DOAJ |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-e03a4af84a13490aad1bd54aa12e2f262025-08-20T02:39:35ZengElsevierEcotoxicology and Environmental Safety0147-65132025-07-0130011845310.1016/j.ecoenv.2025.118453Integration of bioaccumulations, chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic, cadmium and lead in the brown seaweed Sargassum fusiformeYanfang Zhao0Yingying Guo1Jifa Wu2Xuming Kang3Xiaofeng Sheng4Zhijun Tan5Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR ChinaQingdao Gather Great Ocean Seaweed Industry Group, Qingdao 266414, PR ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China; Corresponding author.This study investigated the accumulation and detoxification mechanisms of inorganic arsenic (iAs), cadmium (Cd), and lead (Pb) in Sargassum fusiforme through controlled laboratory exposure experiments combined with chemical speciation analysis and comparative transcriptomics. Our results revealed that S. fusiforme exhibited similar accumulation capacities for arsenite [As(III)] and arsenate [As(V)], with the less toxic As(V) being the predominant species in both treatment groups. The algae demonstrated higher accumulation capacity for Pb compared to Cd. Chemical speciation analysis indicated that S. fusiforme primarily detoxified Cd through organic ligand binding, while Pb was predominantly immobilized as insoluble compounds. Transcriptomic analysis revealed metal-specific detoxification pathways: As(III) exposure significantly enriched pathways related to free radical scavenging, including phenylpropanoid biosynthesis, stilbenoid/diarylheptanoid/gingerol biosynthesis, and flavonoid biosynthesis; As(V) treatment notably affected oxidative phosphorylation among five significantly enriched pathways; Cd exposure triggered complex responses involving 23 significantly enriched pathways; Pb treatment primarily influenced ribosomal biogenesis and translation among four enriched pathways. These findings demonstrated distinct detoxification mechanisms in S. fusiforme for each of the four studied toxic elements.http://www.sciencedirect.com/science/article/pii/S0147651325007936Sargassum fusiformeToxic elementsBioaccumulationDetoxificationChemical formsTranscriptomic analysis |
| spellingShingle | Yanfang Zhao Yingying Guo Jifa Wu Xuming Kang Xiaofeng Sheng Zhijun Tan Integration of bioaccumulations, chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic, cadmium and lead in the brown seaweed Sargassum fusiforme Ecotoxicology and Environmental Safety Sargassum fusiforme Toxic elements Bioaccumulation Detoxification Chemical forms Transcriptomic analysis |
| title | Integration of bioaccumulations, chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic, cadmium and lead in the brown seaweed Sargassum fusiforme |
| title_full | Integration of bioaccumulations, chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic, cadmium and lead in the brown seaweed Sargassum fusiforme |
| title_fullStr | Integration of bioaccumulations, chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic, cadmium and lead in the brown seaweed Sargassum fusiforme |
| title_full_unstemmed | Integration of bioaccumulations, chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic, cadmium and lead in the brown seaweed Sargassum fusiforme |
| title_short | Integration of bioaccumulations, chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic, cadmium and lead in the brown seaweed Sargassum fusiforme |
| title_sort | integration of bioaccumulations chemical forms and gene expression responses to understand the transformation and detoxification of inorganic arsenic cadmium and lead in the brown seaweed sargassum fusiforme |
| topic | Sargassum fusiforme Toxic elements Bioaccumulation Detoxification Chemical forms Transcriptomic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325007936 |
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