Genotyping of ESBL Producing Uropathogenic Escherichia coli in West of Iran
Background and Objective. Urinary tract infection (UTI) is one of the most common bacterial infections in the world. Molecular fingerprinting of UTI isolates such as pulsed-Field Gel Electrophoresis using for Clonal distribution and determine of predominant type. The aim of the study was to determin...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2014/276941 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849467614627102720 |
|---|---|
| author | Parviz Mohajeri Gita Darfarin Abbas Farahani |
| author_facet | Parviz Mohajeri Gita Darfarin Abbas Farahani |
| author_sort | Parviz Mohajeri |
| collection | DOAJ |
| description | Background and Objective. Urinary tract infection (UTI) is one of the most common bacterial infections in the world. Molecular fingerprinting of UTI isolates such as pulsed-Field Gel Electrophoresis using for Clonal distribution and determine of predominant type. The aim of the study was to determine genotyping of ESBL producing UPECs. Material and Methods. 200 UPEC isolates from outpatients with UTI were obtained. Antimicrobial susceptibility and interpretation were performed by disk diffusion. Virulence factors for UPECs were screened by using PCR. UPECs were analyzed by Pulsed-Field Gel Electrophoresis and images analyzed by Phoretix1DPro software. Results. A total of 200 isolates of UPECs, 24.5% (n=49) of isolates, were positive for ESBL production. Resistance ranged from 0% for amikacin and imipenem to over 93.9% for carbenicillin and ampicillin. Frequencies of haemagglutination, haemolysin, and hydrophobicity were 51%, 18.3%, and 14.28%, respectively. A total of 10 different genotypes were obtained, which include nine common clones and one single clone. Conclusion. We confirmed the prevalence of virulence phenotyping especially Haemagglutination among UPEC strains and that it can also contribute to virulence in these strains. Large diversity in genotypes was observed in the isolates that could be indicative of different sources of infection in community acquired. |
| format | Article |
| id | doaj-art-e035ca420c1f46b89120d30cea975a43 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-e035ca420c1f46b89120d30cea975a432025-08-20T03:26:09ZengWileyInternational Journal of Microbiology1687-918X1687-91982014-01-01201410.1155/2014/276941276941Genotyping of ESBL Producing Uropathogenic Escherichia coli in West of IranParviz Mohajeri0Gita Darfarin1Abbas Farahani2Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah 67148-69914, IranDepartment of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, P.O. Box 678, Amol, IranStudent Research Committee, Kermanshah University of Medical Sciences, Kermanshah 67148-69914, IranBackground and Objective. Urinary tract infection (UTI) is one of the most common bacterial infections in the world. Molecular fingerprinting of UTI isolates such as pulsed-Field Gel Electrophoresis using for Clonal distribution and determine of predominant type. The aim of the study was to determine genotyping of ESBL producing UPECs. Material and Methods. 200 UPEC isolates from outpatients with UTI were obtained. Antimicrobial susceptibility and interpretation were performed by disk diffusion. Virulence factors for UPECs were screened by using PCR. UPECs were analyzed by Pulsed-Field Gel Electrophoresis and images analyzed by Phoretix1DPro software. Results. A total of 200 isolates of UPECs, 24.5% (n=49) of isolates, were positive for ESBL production. Resistance ranged from 0% for amikacin and imipenem to over 93.9% for carbenicillin and ampicillin. Frequencies of haemagglutination, haemolysin, and hydrophobicity were 51%, 18.3%, and 14.28%, respectively. A total of 10 different genotypes were obtained, which include nine common clones and one single clone. Conclusion. We confirmed the prevalence of virulence phenotyping especially Haemagglutination among UPEC strains and that it can also contribute to virulence in these strains. Large diversity in genotypes was observed in the isolates that could be indicative of different sources of infection in community acquired.http://dx.doi.org/10.1155/2014/276941 |
| spellingShingle | Parviz Mohajeri Gita Darfarin Abbas Farahani Genotyping of ESBL Producing Uropathogenic Escherichia coli in West of Iran International Journal of Microbiology |
| title | Genotyping of ESBL Producing Uropathogenic Escherichia coli in West of Iran |
| title_full | Genotyping of ESBL Producing Uropathogenic Escherichia coli in West of Iran |
| title_fullStr | Genotyping of ESBL Producing Uropathogenic Escherichia coli in West of Iran |
| title_full_unstemmed | Genotyping of ESBL Producing Uropathogenic Escherichia coli in West of Iran |
| title_short | Genotyping of ESBL Producing Uropathogenic Escherichia coli in West of Iran |
| title_sort | genotyping of esbl producing uropathogenic escherichia coli in west of iran |
| url | http://dx.doi.org/10.1155/2014/276941 |
| work_keys_str_mv | AT parvizmohajeri genotypingofesblproducinguropathogenicescherichiacoliinwestofiran AT gitadarfarin genotypingofesblproducinguropathogenicescherichiacoliinwestofiran AT abbasfarahani genotypingofesblproducinguropathogenicescherichiacoliinwestofiran |