Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves
Carissa edulis is a plant used in the management of oxidative stress and inflammatory related disorders such as malaria, rheumatism inflammation, and cardiovascular diseases. The present study evaluates the total phenolic content, antioxidant capacity (DPPH, ABTS, and FRAP), and the bioactive compou...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2019/7549620 |
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| author | Sabine Adeline Fanta Yadang Germain Taiwe Sotoing Katoucha Sidoine Ngatcha Zouakeu Muhammad Ahsan Khan Gabriel Agbor Agbor Nisar Ur-Rahman Elisabeth Ngo Bum |
| author_facet | Sabine Adeline Fanta Yadang Germain Taiwe Sotoing Katoucha Sidoine Ngatcha Zouakeu Muhammad Ahsan Khan Gabriel Agbor Agbor Nisar Ur-Rahman Elisabeth Ngo Bum |
| author_sort | Sabine Adeline Fanta Yadang |
| collection | DOAJ |
| description | Carissa edulis is a plant used in the management of oxidative stress and inflammatory related disorders such as malaria, rheumatism inflammation, and cardiovascular diseases. The present study evaluates the total phenolic content, antioxidant capacity (DPPH, ABTS, and FRAP), and the bioactive compounds present in the various extracts of C. edulis (HEC, MEC, AEC, and PC). An HPLC analysis determined the different compounds present in the extracts. High concentration of total phenolic content was observed in aqueous and methanolic extracts more than in the hydroethanolic extract though not significantly different. Flavonoids were higher in the hydroethanolic and methanolic extracts, respectively, with 14.84 mg RE/g extract and 12.02 mg RE/g extract. Tannins were also found in large amounts in the same two extracts with 26.76 mg TAE/g extract and 34.67 mg TEE/g extract. The percentage radical scavenging activity DPPH ranged between 58.63% and 94.67% for aqueous extract and for ABTS between 51.39% and 94.12% for the methanolic extract. The highest FRAP was obtained in the methanolic extract (6.73 g AAE/100 g extract). HPLC analysis revealed the presence of quercetin, rutin, and gallic acid in the different extracts. C. edulis represents a potential source of bioactive components with antioxidant capacity. |
| format | Article |
| id | doaj-art-e0285df4800945b0a4ecd5924f63b642 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e0285df4800945b0a4ecd5924f63b6422025-08-20T02:21:07ZengWileyThe Scientific World Journal2356-61401537-744X2019-01-01201910.1155/2019/75496207549620Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) LeavesSabine Adeline Fanta Yadang0Germain Taiwe Sotoing1Katoucha Sidoine Ngatcha Zouakeu2Muhammad Ahsan Khan3Gabriel Agbor Agbor4Nisar Ur-Rahman5Elisabeth Ngo Bum6Institute of Medical Research and Medicinal Plants Studies, Ministry of Scientific Research and Innovation, Yaoundé, CameroonDepartment of Zoology and Animal Physiology, Faculty of Science, University of Buea, CameroonDepartment of Biological Sciences, Faculty of Science, University of Ngaoundere, CameroonDepartment of Pharmacy, Comsats University Islamabad, Abbottabad Campus, PakistanInstitute of Medical Research and Medicinal Plants Studies, Ministry of Scientific Research and Innovation, Yaoundé, CameroonDepartment of Pharmacy, Comsats University Islamabad, Abbottabad Campus, PakistanDepartment of Biological Sciences, Faculty of Science, University of Ngaoundere, CameroonCarissa edulis is a plant used in the management of oxidative stress and inflammatory related disorders such as malaria, rheumatism inflammation, and cardiovascular diseases. The present study evaluates the total phenolic content, antioxidant capacity (DPPH, ABTS, and FRAP), and the bioactive compounds present in the various extracts of C. edulis (HEC, MEC, AEC, and PC). An HPLC analysis determined the different compounds present in the extracts. High concentration of total phenolic content was observed in aqueous and methanolic extracts more than in the hydroethanolic extract though not significantly different. Flavonoids were higher in the hydroethanolic and methanolic extracts, respectively, with 14.84 mg RE/g extract and 12.02 mg RE/g extract. Tannins were also found in large amounts in the same two extracts with 26.76 mg TAE/g extract and 34.67 mg TEE/g extract. The percentage radical scavenging activity DPPH ranged between 58.63% and 94.67% for aqueous extract and for ABTS between 51.39% and 94.12% for the methanolic extract. The highest FRAP was obtained in the methanolic extract (6.73 g AAE/100 g extract). HPLC analysis revealed the presence of quercetin, rutin, and gallic acid in the different extracts. C. edulis represents a potential source of bioactive components with antioxidant capacity.http://dx.doi.org/10.1155/2019/7549620 |
| spellingShingle | Sabine Adeline Fanta Yadang Germain Taiwe Sotoing Katoucha Sidoine Ngatcha Zouakeu Muhammad Ahsan Khan Gabriel Agbor Agbor Nisar Ur-Rahman Elisabeth Ngo Bum Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves The Scientific World Journal |
| title | Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves |
| title_full | Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves |
| title_fullStr | Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves |
| title_full_unstemmed | Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves |
| title_short | Quantification of Bioactive Compounds and Evaluation of the Antioxidant Activity of Carissa edulis Valh (Apocynaceae) Leaves |
| title_sort | quantification of bioactive compounds and evaluation of the antioxidant activity of carissa edulis valh apocynaceae leaves |
| url | http://dx.doi.org/10.1155/2019/7549620 |
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