PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway
Abstract Background Renal cell carcinoma (RCC) is a frequent and aggressive type of kidney cancer with limited therapeutic options. Although phosphatase and tensin homolog (PTEN) have been recognized as a potential tumor suppressor in all kinds of cancers, its function in RCC remains to be thoroughl...
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| Language: | English |
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BMC
2025-02-01
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| Series: | World Journal of Surgical Oncology |
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| Online Access: | https://doi.org/10.1186/s12957-025-03658-9 |
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| author | Xu Xu Yuan-Yue Tang Xiaohong Liang Wen Luo Dong-Mei Jiang Jie Chen |
| author_facet | Xu Xu Yuan-Yue Tang Xiaohong Liang Wen Luo Dong-Mei Jiang Jie Chen |
| author_sort | Xu Xu |
| collection | DOAJ |
| description | Abstract Background Renal cell carcinoma (RCC) is a frequent and aggressive type of kidney cancer with limited therapeutic options. Although phosphatase and tensin homolog (PTEN) have been recognized as a potential tumor suppressor in all kinds of cancers, its function in RCC remains to be thoroughly elucidated. Objective This article was recruited to examine the PTEN’s role in managing the PI3K/AKT pathway and its impact on the RCC cell proliferation and migration. Methods This study collected renal cancer and adjacent non-cancerous tissue samples from our hospital. HK-2 and 786-O cells were used, with 786-O cells divided into control, vector, and oe-PTEN groups. PTEN and related protein levels were detected using RT-qPCR and Western blot. Statistical analyses were performed using the Mann-Whitney U test and Kruskal-Wallis H test. Cell viability and migration were assessed using the CCK-8 assay and wound healing assay. All analyses were conducted with SPSS 22.0 software, with statistical significance defined as p < 0.05. Results RT-qPCR results showed that PTEN expression was significantly increased in RCC tumor tissues compared to normal tissues (p < 0.01). However, PTEN mRNA levels were significantly reduced in 786-O cells compared to HK-2 cells (p < 0.01). In 786-O cells with low PTEN expression, further induction of PTEN overexpression significantly inhibited PI3K/AKT signaling activity (p < 0.01), accompanied by decreased cell viability and migration ability. These results indicate that the expression pattern of PTEN in RCC is complex, but its overexpression can exert tumor-suppressive effects by inhibiting the PI3K/AKT signaling pathway. Conclusion Our findings demonstrate that PTEN overexpression in RCC cells leads to decreased PI3K/AKT signaling, decreasing cell viability and migration. This study highlights the critical role of PTEN in RCC progression and suggests potential therapeutic targets for intervention. |
| format | Article |
| id | doaj-art-e019a7efb8b345029f5dd64dbc0b1d29 |
| institution | Kabale University |
| issn | 1477-7819 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj-art-e019a7efb8b345029f5dd64dbc0b1d292025-02-09T12:39:56ZengBMCWorld Journal of Surgical Oncology1477-78192025-02-012311810.1186/s12957-025-03658-9PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathwayXu Xu0Yuan-Yue Tang1Xiaohong Liang2Wen Luo3Dong-Mei Jiang4Jie Chen5Department of Haematology, The First Affiliated Hospital of Naval Medical University, No.168 Changhai Road, Yangpu DistrictDepartment of Haematology, The First Affiliated Hospital of Naval Medical University, No.168 Changhai Road, Yangpu DistrictDepartment of Urology, Fudan University Shanghai Cancer Center, No. 270 Dongan Road, Xuhui DistrictDepartment of Haematology, The First Affiliated Hospital of Naval Medical University, No.168 Changhai Road, Yangpu DistrictDepartment of Haematology, The First Affiliated Hospital of Naval Medical University, No.168 Changhai Road, Yangpu DistrictDepartment of Haematology, The First Affiliated Hospital of Naval Medical University, No.168 Changhai Road, Yangpu DistrictAbstract Background Renal cell carcinoma (RCC) is a frequent and aggressive type of kidney cancer with limited therapeutic options. Although phosphatase and tensin homolog (PTEN) have been recognized as a potential tumor suppressor in all kinds of cancers, its function in RCC remains to be thoroughly elucidated. Objective This article was recruited to examine the PTEN’s role in managing the PI3K/AKT pathway and its impact on the RCC cell proliferation and migration. Methods This study collected renal cancer and adjacent non-cancerous tissue samples from our hospital. HK-2 and 786-O cells were used, with 786-O cells divided into control, vector, and oe-PTEN groups. PTEN and related protein levels were detected using RT-qPCR and Western blot. Statistical analyses were performed using the Mann-Whitney U test and Kruskal-Wallis H test. Cell viability and migration were assessed using the CCK-8 assay and wound healing assay. All analyses were conducted with SPSS 22.0 software, with statistical significance defined as p < 0.05. Results RT-qPCR results showed that PTEN expression was significantly increased in RCC tumor tissues compared to normal tissues (p < 0.01). However, PTEN mRNA levels were significantly reduced in 786-O cells compared to HK-2 cells (p < 0.01). In 786-O cells with low PTEN expression, further induction of PTEN overexpression significantly inhibited PI3K/AKT signaling activity (p < 0.01), accompanied by decreased cell viability and migration ability. These results indicate that the expression pattern of PTEN in RCC is complex, but its overexpression can exert tumor-suppressive effects by inhibiting the PI3K/AKT signaling pathway. Conclusion Our findings demonstrate that PTEN overexpression in RCC cells leads to decreased PI3K/AKT signaling, decreasing cell viability and migration. This study highlights the critical role of PTEN in RCC progression and suggests potential therapeutic targets for intervention.https://doi.org/10.1186/s12957-025-03658-9PTENPI3K/AKT pathwayRenal cell carcinomaCell proliferationCell migration |
| spellingShingle | Xu Xu Yuan-Yue Tang Xiaohong Liang Wen Luo Dong-Mei Jiang Jie Chen PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway World Journal of Surgical Oncology PTEN PI3K/AKT pathway Renal cell carcinoma Cell proliferation Cell migration |
| title | PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway |
| title_full | PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway |
| title_fullStr | PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway |
| title_full_unstemmed | PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway |
| title_short | PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway |
| title_sort | pten suppresses renal cell carcinoma proliferation and migration via inhibition of the pi3k akt pathway |
| topic | PTEN PI3K/AKT pathway Renal cell carcinoma Cell proliferation Cell migration |
| url | https://doi.org/10.1186/s12957-025-03658-9 |
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