The Role of Type 2 Innate Lymphoid Cells in Adenoid Hypertrophy with Allergic Rhinitis Among Children and Related Potential Therapeutic Targets
Yan Hao,1– 3 Tian-Yong Hu,3 Mei-Zhen Zhao,3 Xian-Hai Zeng,3 Ke Li,3 Bao-Hui Cheng,3 Da-Bo Liu1,2 1Department of Pediatric Otolaryngology, Shenzhen Hospital, Southern Medical University, Shenzhen, People’s Republic of China; 2The Third School of Clinical Medicine, Southern Medical University, Guangzh...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-07-01
|
| Series: | Journal of Inflammation Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/the-role-of-type-2-innate-lymphoid-cells-in-adenoid-hypertrophy-with-a-peer-reviewed-fulltext-article-JIR |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849422024183644160 |
|---|---|
| author | Hao Y Hu TY Zhao MZ Zeng XH Li K Cheng BH Liu DB |
| author_facet | Hao Y Hu TY Zhao MZ Zeng XH Li K Cheng BH Liu DB |
| author_sort | Hao Y |
| collection | DOAJ |
| description | Yan Hao,1– 3 Tian-Yong Hu,3 Mei-Zhen Zhao,3 Xian-Hai Zeng,3 Ke Li,3 Bao-Hui Cheng,3 Da-Bo Liu1,2 1Department of Pediatric Otolaryngology, Shenzhen Hospital, Southern Medical University, Shenzhen, People’s Republic of China; 2The Third School of Clinical Medicine, Southern Medical University, Guangzhou, People’s Republic of China; 3Department of Otolaryngology, Shenzhen Longgang Otolaryngology Hospital & Shenzhen Otolaryngology Research Institute, Shenzhen, People’s Republic of ChinaCorrespondence: Bao-Hui Cheng, Email chengbaohui@sina.com Da-Bo Liu, Email daboliu@mailnesia.comObjective: Innate lymphoid cells (ILC) are a heterogeneous group of immune cells implicated in immune diseases. However, their specific roles in adenoid hypertrophy (AH), AH with allergic rhinitis (AH+AR), and AH with otitis media with effusion (AH+OME) remain poorly understood. This study aimed to characterize ILC subsets and their association with immunological profiles in these conditions.Methods: Flow cytometry was used to quantify ILC subsets in adenoid tissues from patients with AH, AH+AR, or AH+OME, and correlations between ILC subsets and clinical, immunological (serum and tissue cytokines), and histopathological parameters were further assessed.Results: ST2 mRNA and protein expression were significantly higher in AH+AR than in AH and AH+OME (p < 0.05). Serum IL-33 was elevated in AH+AR compared to AH (p = 0.0127), while IFN-γ was higher in AH than in AH+AR (p = 0.0044). IL-4 levels were higher in AH and AH+AR than in AH+OME (p < 0.005). Flow cytometry showed that ILC2 predominated in AH+AR (p = 0.0009 vs AH+OME), with higher ILC2/ILC1 and ILC2/ILC3 ratios in AH and AH+AR compared to AH+OME (p < 0.05). Correlation analysis indicated that ILC2 in AH+AR positively correlated with serum IgE, IL-4, IL-33, thymic stromal lymphopoietin (TSLP), and tissue IL-4, IL-33, TSLP, and IL-25 (p < 0.05). ILC3 inversely correlated with peripheral blood eosinophils (p = 0.0125) and positively with serum and tissue IL-17A (p < 0.05).Conclusion: ILC2 cells are significantly enriched in adenoid tissues of patients with AH+AR, with elevated ST2 and IL-33 levels supporting the activation of the IL-33/ST2/ILC2 signaling pathway. Targeting this pathway may offer novel therapeutic strategies for AH combined with allergic rhinitis.Keywords: adenoid hypertrophy, innate lymphoid cells, allergic rhinitis, otitis media with effusion, group 2 innate lymphoid cell |
| format | Article |
| id | doaj-art-e005167a310b407f8f8279eb5daf1a88 |
| institution | Kabale University |
| issn | 1178-7031 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj-art-e005167a310b407f8f8279eb5daf1a882025-08-20T03:31:16ZengDove Medical PressJournal of Inflammation Research1178-70312025-07-01Volume 18Issue 185938605104390The Role of Type 2 Innate Lymphoid Cells in Adenoid Hypertrophy with Allergic Rhinitis Among Children and Related Potential Therapeutic TargetsHao Y0Hu TYZhao MZZeng XHLi KCheng BH1Liu DBDepartment of Pediatric OtolaryngologyDepartment of OtolaryngologyYan Hao,1– 3 Tian-Yong Hu,3 Mei-Zhen Zhao,3 Xian-Hai Zeng,3 Ke Li,3 Bao-Hui Cheng,3 Da-Bo Liu1,2 1Department of Pediatric Otolaryngology, Shenzhen Hospital, Southern Medical University, Shenzhen, People’s Republic of China; 2The Third School of Clinical Medicine, Southern Medical University, Guangzhou, People’s Republic of China; 3Department of Otolaryngology, Shenzhen Longgang Otolaryngology Hospital & Shenzhen Otolaryngology Research Institute, Shenzhen, People’s Republic of ChinaCorrespondence: Bao-Hui Cheng, Email chengbaohui@sina.com Da-Bo Liu, Email daboliu@mailnesia.comObjective: Innate lymphoid cells (ILC) are a heterogeneous group of immune cells implicated in immune diseases. However, their specific roles in adenoid hypertrophy (AH), AH with allergic rhinitis (AH+AR), and AH with otitis media with effusion (AH+OME) remain poorly understood. This study aimed to characterize ILC subsets and their association with immunological profiles in these conditions.Methods: Flow cytometry was used to quantify ILC subsets in adenoid tissues from patients with AH, AH+AR, or AH+OME, and correlations between ILC subsets and clinical, immunological (serum and tissue cytokines), and histopathological parameters were further assessed.Results: ST2 mRNA and protein expression were significantly higher in AH+AR than in AH and AH+OME (p < 0.05). Serum IL-33 was elevated in AH+AR compared to AH (p = 0.0127), while IFN-γ was higher in AH than in AH+AR (p = 0.0044). IL-4 levels were higher in AH and AH+AR than in AH+OME (p < 0.005). Flow cytometry showed that ILC2 predominated in AH+AR (p = 0.0009 vs AH+OME), with higher ILC2/ILC1 and ILC2/ILC3 ratios in AH and AH+AR compared to AH+OME (p < 0.05). Correlation analysis indicated that ILC2 in AH+AR positively correlated with serum IgE, IL-4, IL-33, thymic stromal lymphopoietin (TSLP), and tissue IL-4, IL-33, TSLP, and IL-25 (p < 0.05). ILC3 inversely correlated with peripheral blood eosinophils (p = 0.0125) and positively with serum and tissue IL-17A (p < 0.05).Conclusion: ILC2 cells are significantly enriched in adenoid tissues of patients with AH+AR, with elevated ST2 and IL-33 levels supporting the activation of the IL-33/ST2/ILC2 signaling pathway. Targeting this pathway may offer novel therapeutic strategies for AH combined with allergic rhinitis.Keywords: adenoid hypertrophy, innate lymphoid cells, allergic rhinitis, otitis media with effusion, group 2 innate lymphoid cellhttps://www.dovepress.com/the-role-of-type-2-innate-lymphoid-cells-in-adenoid-hypertrophy-with-a-peer-reviewed-fulltext-article-JIRadenoid hypertrophyinnate lymphoid cellsallergic rhinitisotitis media with effusiongroup 2 innate lymphoid cell |
| spellingShingle | Hao Y Hu TY Zhao MZ Zeng XH Li K Cheng BH Liu DB The Role of Type 2 Innate Lymphoid Cells in Adenoid Hypertrophy with Allergic Rhinitis Among Children and Related Potential Therapeutic Targets Journal of Inflammation Research adenoid hypertrophy innate lymphoid cells allergic rhinitis otitis media with effusion group 2 innate lymphoid cell |
| title | The Role of Type 2 Innate Lymphoid Cells in Adenoid Hypertrophy with Allergic Rhinitis Among Children and Related Potential Therapeutic Targets |
| title_full | The Role of Type 2 Innate Lymphoid Cells in Adenoid Hypertrophy with Allergic Rhinitis Among Children and Related Potential Therapeutic Targets |
| title_fullStr | The Role of Type 2 Innate Lymphoid Cells in Adenoid Hypertrophy with Allergic Rhinitis Among Children and Related Potential Therapeutic Targets |
| title_full_unstemmed | The Role of Type 2 Innate Lymphoid Cells in Adenoid Hypertrophy with Allergic Rhinitis Among Children and Related Potential Therapeutic Targets |
| title_short | The Role of Type 2 Innate Lymphoid Cells in Adenoid Hypertrophy with Allergic Rhinitis Among Children and Related Potential Therapeutic Targets |
| title_sort | role of type 2 innate lymphoid cells in adenoid hypertrophy with allergic rhinitis among children and related potential therapeutic targets |
| topic | adenoid hypertrophy innate lymphoid cells allergic rhinitis otitis media with effusion group 2 innate lymphoid cell |
| url | https://www.dovepress.com/the-role-of-type-2-innate-lymphoid-cells-in-adenoid-hypertrophy-with-a-peer-reviewed-fulltext-article-JIR |
| work_keys_str_mv | AT haoy theroleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT huty theroleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT zhaomz theroleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT zengxh theroleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT lik theroleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT chengbh theroleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT liudb theroleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT haoy roleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT huty roleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT zhaomz roleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT zengxh roleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT lik roleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT chengbh roleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets AT liudb roleoftype2innatelymphoidcellsinadenoidhypertrophywithallergicrhinitisamongchildrenandrelatedpotentialtherapeutictargets |