Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients
Background. Taurine transporter gene expression (RNA-TauT) has a role in retinal cell function and is modulated in vitro and in vivo by hyperglycemia and/or oxidative stress. This study was aimed at testing whether RNA-TauT gene expression is modified in blood mononuclear peripheral cells (MPCs) of...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/7313162 |
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| author | Zaleida Napoli Giuseppe Seghieri Loria Bianchi Roberto Anichini Alessandra De Bellis Ilaria Campesi Ciriaco Carru Stefano Occhioni Angelo Zinellu Flavia Franconi |
| author_facet | Zaleida Napoli Giuseppe Seghieri Loria Bianchi Roberto Anichini Alessandra De Bellis Ilaria Campesi Ciriaco Carru Stefano Occhioni Angelo Zinellu Flavia Franconi |
| author_sort | Zaleida Napoli |
| collection | DOAJ |
| description | Background. Taurine transporter gene expression (RNA-TauT) has a role in retinal cell function and is modulated in vitro and in vivo by hyperglycemia and/or oxidative stress. This study was aimed at testing whether RNA-TauT gene expression is modified in blood mononuclear peripheral cells (MPCs) of type 1 diabetic patients, is related to plasma markers of oxidative stress or endothelial dysfunction, or, finally, is related to presence of retinopathy. Methods. RNA-TauT was measured in MPCs by real-time PCR-analysis in 35 type 1 diabetic patients and in 33 age- and sex-matched controls, additionally measuring plasma and cell taurine and markers of oxidative stress and endothelial dysfunction. Results. RNA-TauT, expressed as 2-ΔΔCt, was significantly higher in MPCs of type 1 diabetic patients than in controls [median (interquartile range): 1.32(0.31) versus 1.00(0.15); P=0.01]. In diabetic patients RNA-TauT was related to HbA1c (r=0.42; P=0.01) and inversely to plasma homocysteine (r=-0.39; P=0.02) being additionally significantly higher in MPCs of patients without retinopathy [(n=22); 1.36(0.34)] compared to those with retinopathy [(n=13); 1.16(0.20)], independently from HbA1c or diabetes duration. Conclusions. RNA-TauT gene expression is significantly upregulated in MPCs of type 1 diabetes patients and is related to HbA1c levels and inversely to plasma homocysteine. Finally, in diabetes patients, RNA-TauT upregulation seems to be blunted in patients with retinopathy independently of their metabolic control or longer diabetes duration. |
| format | Article |
| id | doaj-art-e0031f9a553846b49044bdbe1158da98 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-e0031f9a553846b49044bdbe1158da982025-08-20T03:26:05ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/73131627313162Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes PatientsZaleida Napoli0Giuseppe Seghieri1Loria Bianchi2Roberto Anichini3Alessandra De Bellis4Ilaria Campesi5Ciriaco Carru6Stefano Occhioni7Angelo Zinellu8Flavia Franconi9Department of Clinical Chemistry, S. Jacopo Hospital, 51100 Pistoia, ItalyAgenzia Regionale Sanità, Toscana, Florence, ItalyDepartment of Clinical Chemistry, S. Jacopo Hospital, 51100 Pistoia, ItalyDiabetes Unit, S. Jacopo Hospital, 51100 Pistoia, ItalyDiabetes Unit, S. Jacopo Hospital, 51100 Pistoia, ItalyDepartment of Biomedical Sciences, University of Sassari, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, 07100 Sassari, ItalyDepartment of Biomedical Sciences, University of Sassari, 07100 Sassari, ItalyBackground. Taurine transporter gene expression (RNA-TauT) has a role in retinal cell function and is modulated in vitro and in vivo by hyperglycemia and/or oxidative stress. This study was aimed at testing whether RNA-TauT gene expression is modified in blood mononuclear peripheral cells (MPCs) of type 1 diabetic patients, is related to plasma markers of oxidative stress or endothelial dysfunction, or, finally, is related to presence of retinopathy. Methods. RNA-TauT was measured in MPCs by real-time PCR-analysis in 35 type 1 diabetic patients and in 33 age- and sex-matched controls, additionally measuring plasma and cell taurine and markers of oxidative stress and endothelial dysfunction. Results. RNA-TauT, expressed as 2-ΔΔCt, was significantly higher in MPCs of type 1 diabetic patients than in controls [median (interquartile range): 1.32(0.31) versus 1.00(0.15); P=0.01]. In diabetic patients RNA-TauT was related to HbA1c (r=0.42; P=0.01) and inversely to plasma homocysteine (r=-0.39; P=0.02) being additionally significantly higher in MPCs of patients without retinopathy [(n=22); 1.36(0.34)] compared to those with retinopathy [(n=13); 1.16(0.20)], independently from HbA1c or diabetes duration. Conclusions. RNA-TauT gene expression is significantly upregulated in MPCs of type 1 diabetes patients and is related to HbA1c levels and inversely to plasma homocysteine. Finally, in diabetes patients, RNA-TauT upregulation seems to be blunted in patients with retinopathy independently of their metabolic control or longer diabetes duration.http://dx.doi.org/10.1155/2016/7313162 |
| spellingShingle | Zaleida Napoli Giuseppe Seghieri Loria Bianchi Roberto Anichini Alessandra De Bellis Ilaria Campesi Ciriaco Carru Stefano Occhioni Angelo Zinellu Flavia Franconi Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients Journal of Diabetes Research |
| title | Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients |
| title_full | Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients |
| title_fullStr | Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients |
| title_full_unstemmed | Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients |
| title_short | Taurine Transporter Gene Expression in Mononuclear Blood Cells of Type 1 Diabetes Patients |
| title_sort | taurine transporter gene expression in mononuclear blood cells of type 1 diabetes patients |
| url | http://dx.doi.org/10.1155/2016/7313162 |
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