The BioFire® RP2.1 Panel Did Not Identify Concurrent Respiratory Virus Infection in Adults with Variable SARS-CoV-2 Disease Severity and Infection Duration
SARS-CoV-2 emerged in 2019 and rapidly surged into a global pandemic. The rates of concurrent infection with other respiratory pathogens and the effects of possible coinfections on the severity of COVID-19 cases and the length of viral infection are not well defined. In this retrospective study, nas...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2022/1378482 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850167842913124352 |
|---|---|
| author | Kendra M. Quicke Bridget A. Baxter Sophia Stromberg Emily N. Gallichotte Emily Fitzmeyer Michael C. Young Kristy L. Pabilonia Nicole Ehrhart Julie Dunn Gregory D. Ebel Elizabeth P. Ryan |
| author_facet | Kendra M. Quicke Bridget A. Baxter Sophia Stromberg Emily N. Gallichotte Emily Fitzmeyer Michael C. Young Kristy L. Pabilonia Nicole Ehrhart Julie Dunn Gregory D. Ebel Elizabeth P. Ryan |
| author_sort | Kendra M. Quicke |
| collection | DOAJ |
| description | SARS-CoV-2 emerged in 2019 and rapidly surged into a global pandemic. The rates of concurrent infection with other respiratory pathogens and the effects of possible coinfections on the severity of COVID-19 cases and the length of viral infection are not well defined. In this retrospective study, nasopharyngeal swab samples collected in Colorado between March 2020 and May 2021 from SARS-CoV-2 PCR-positive individuals were tested for a panel of 21 additional respiratory pathogens, including 17 viral and 4 bacterial pathogens. We detected significant positive correlations between levels of SARS-CoV-2 RNA and infectious virus titers for both cohorts, as well as a positive correlation between viral RNA levels and disease severity scores for one cohort. We hypothesized that severe COVID-19 cases and longer SARS-CoV-2 infections may be associated with concurrent respiratory infections. Only one individual exhibited evidence of a concurrent infection- SARS -CoV-2 and human rhinovirus/enterovirus- leading us to conclude that viral respiratory coinfections were uncommon during this time and thus not responsible for the variations in disease severity and infection duration observed in the two cohorts examined. Mask wearing and other public health measures were imposed in Colorado during the time of collection and likely contributed to low rates of coinfection. |
| format | Article |
| id | doaj-art-dfeb9ba7b8d94d1f969d0252857621b7 |
| institution | OA Journals |
| issn | 1687-8647 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-dfeb9ba7b8d94d1f969d0252857621b72025-08-20T02:21:07ZengWileyAdvances in Virology1687-86472022-01-01202210.1155/2022/1378482The BioFire® RP2.1 Panel Did Not Identify Concurrent Respiratory Virus Infection in Adults with Variable SARS-CoV-2 Disease Severity and Infection DurationKendra M. Quicke0Bridget A. Baxter1Sophia Stromberg2Emily N. Gallichotte3Emily Fitzmeyer4Michael C. Young5Kristy L. Pabilonia6Nicole Ehrhart7Julie Dunn8Gregory D. Ebel9Elizabeth P. Ryan10Arthropod-Borne and Infectious Diseases LaboratoryDepartment of Environmental and Radiological Health SciencesDepartment of Environmental and Radiological Health SciencesArthropod-Borne and Infectious Diseases LaboratoryArthropod-Borne and Infectious Diseases LaboratoryArthropod-Borne and Infectious Diseases LaboratoryArthropod-Borne and Infectious Diseases LaboratoryColumbine Health Systems Center for Healthy Aging and Department of Clinical SciencesUniversity of Colorado HealthArthropod-Borne and Infectious Diseases LaboratoryDepartment of Environmental and Radiological Health SciencesSARS-CoV-2 emerged in 2019 and rapidly surged into a global pandemic. The rates of concurrent infection with other respiratory pathogens and the effects of possible coinfections on the severity of COVID-19 cases and the length of viral infection are not well defined. In this retrospective study, nasopharyngeal swab samples collected in Colorado between March 2020 and May 2021 from SARS-CoV-2 PCR-positive individuals were tested for a panel of 21 additional respiratory pathogens, including 17 viral and 4 bacterial pathogens. We detected significant positive correlations between levels of SARS-CoV-2 RNA and infectious virus titers for both cohorts, as well as a positive correlation between viral RNA levels and disease severity scores for one cohort. We hypothesized that severe COVID-19 cases and longer SARS-CoV-2 infections may be associated with concurrent respiratory infections. Only one individual exhibited evidence of a concurrent infection- SARS -CoV-2 and human rhinovirus/enterovirus- leading us to conclude that viral respiratory coinfections were uncommon during this time and thus not responsible for the variations in disease severity and infection duration observed in the two cohorts examined. Mask wearing and other public health measures were imposed in Colorado during the time of collection and likely contributed to low rates of coinfection.http://dx.doi.org/10.1155/2022/1378482 |
| spellingShingle | Kendra M. Quicke Bridget A. Baxter Sophia Stromberg Emily N. Gallichotte Emily Fitzmeyer Michael C. Young Kristy L. Pabilonia Nicole Ehrhart Julie Dunn Gregory D. Ebel Elizabeth P. Ryan The BioFire® RP2.1 Panel Did Not Identify Concurrent Respiratory Virus Infection in Adults with Variable SARS-CoV-2 Disease Severity and Infection Duration Advances in Virology |
| title | The BioFire® RP2.1 Panel Did Not Identify Concurrent Respiratory Virus Infection in Adults with Variable SARS-CoV-2 Disease Severity and Infection Duration |
| title_full | The BioFire® RP2.1 Panel Did Not Identify Concurrent Respiratory Virus Infection in Adults with Variable SARS-CoV-2 Disease Severity and Infection Duration |
| title_fullStr | The BioFire® RP2.1 Panel Did Not Identify Concurrent Respiratory Virus Infection in Adults with Variable SARS-CoV-2 Disease Severity and Infection Duration |
| title_full_unstemmed | The BioFire® RP2.1 Panel Did Not Identify Concurrent Respiratory Virus Infection in Adults with Variable SARS-CoV-2 Disease Severity and Infection Duration |
| title_short | The BioFire® RP2.1 Panel Did Not Identify Concurrent Respiratory Virus Infection in Adults with Variable SARS-CoV-2 Disease Severity and Infection Duration |
| title_sort | biofire r rp2 1 panel did not identify concurrent respiratory virus infection in adults with variable sars cov 2 disease severity and infection duration |
| url | http://dx.doi.org/10.1155/2022/1378482 |
| work_keys_str_mv | AT kendramquicke thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT bridgetabaxter thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT sophiastromberg thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT emilyngallichotte thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT emilyfitzmeyer thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT michaelcyoung thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT kristylpabilonia thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT nicoleehrhart thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT juliedunn thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT gregorydebel thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT elizabethpryan thebiofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT kendramquicke biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT bridgetabaxter biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT sophiastromberg biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT emilyngallichotte biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT emilyfitzmeyer biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT michaelcyoung biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT kristylpabilonia biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT nicoleehrhart biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT juliedunn biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT gregorydebel biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration AT elizabethpryan biofirerp21paneldidnotidentifyconcurrentrespiratoryvirusinfectioninadultswithvariablesarscov2diseaseseverityandinfectionduration |