Enhancing nutritional niche and host defenses by modifying the gut microbiome

Abstract The gut microbiome is essential for processing complex food compounds and synthesizing nutrients that the host cannot digest or produce, respectively. New model systems are needed to study how the metabolic capacity provided by the gut microbiome impacts the nutritional status of the host,...

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Main Authors: Qing Sun, Nic M Vega, Bernardo Cervantes, Christopher P Mancuso, Ning Mao, Megan N Taylor, James J Collins, Ahmad S Khalil, Jeff Gore, Timothy K Lu
Format: Article
Language:English
Published: Springer Nature 2022-11-01
Series:Molecular Systems Biology
Subjects:
Online Access:https://doi.org/10.15252/msb.20209933
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author Qing Sun
Nic M Vega
Bernardo Cervantes
Christopher P Mancuso
Ning Mao
Megan N Taylor
James J Collins
Ahmad S Khalil
Jeff Gore
Timothy K Lu
author_facet Qing Sun
Nic M Vega
Bernardo Cervantes
Christopher P Mancuso
Ning Mao
Megan N Taylor
James J Collins
Ahmad S Khalil
Jeff Gore
Timothy K Lu
author_sort Qing Sun
collection DOAJ
description Abstract The gut microbiome is essential for processing complex food compounds and synthesizing nutrients that the host cannot digest or produce, respectively. New model systems are needed to study how the metabolic capacity provided by the gut microbiome impacts the nutritional status of the host, and to explore possibilities for altering host metabolic capacity via the microbiome. Here, we colonized the nematode Caenorhabditis elegans gut with cellulolytic bacteria that enabled C. elegans to utilize cellulose, an otherwise indigestible substrate, as a carbon source. Cellulolytic bacteria as a community component in the worm gut can also support additional bacterial species with specialized roles, which we demonstrate by using Lactobacillus plantarum to protect C. elegans against Salmonella enterica infection. This work shows that engineered microbiome communities can be used to endow host organisms with novel functions, such as the ability to utilize alternate nutrient sources or to better fight pathogenic bacteria.
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institution Kabale University
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publisher Springer Nature
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series Molecular Systems Biology
spelling doaj-art-dfd753b767f0430fb0906fcbd549bb7e2025-08-24T12:01:03ZengSpringer NatureMolecular Systems Biology1744-42922022-11-01181111110.15252/msb.20209933Enhancing nutritional niche and host defenses by modifying the gut microbiomeQing Sun0Nic M Vega1Bernardo Cervantes2Christopher P Mancuso3Ning Mao4Megan N Taylor5James J Collins6Ahmad S Khalil7Jeff Gore8Timothy K Lu9Synthetic Biology Center, MITDepartment of Physics, MITInstitute for Medical Engineering & Science and Department of Biological Engineering, MITBiological Design Center, Boston UniversityDepartment of Biomedical Engineering, Boston UniversityBiology Department, Emory UniversitySynthetic Biology Center, MITBiological Design Center, Boston UniversityDepartment of Physics, MITSynthetic Biology Center, MITAbstract The gut microbiome is essential for processing complex food compounds and synthesizing nutrients that the host cannot digest or produce, respectively. New model systems are needed to study how the metabolic capacity provided by the gut microbiome impacts the nutritional status of the host, and to explore possibilities for altering host metabolic capacity via the microbiome. Here, we colonized the nematode Caenorhabditis elegans gut with cellulolytic bacteria that enabled C. elegans to utilize cellulose, an otherwise indigestible substrate, as a carbon source. Cellulolytic bacteria as a community component in the worm gut can also support additional bacterial species with specialized roles, which we demonstrate by using Lactobacillus plantarum to protect C. elegans against Salmonella enterica infection. This work shows that engineered microbiome communities can be used to endow host organisms with novel functions, such as the ability to utilize alternate nutrient sources or to better fight pathogenic bacteria.https://doi.org/10.15252/msb.20209933bacteria communitycellulosegut microbiomenutritionpathogen
spellingShingle Qing Sun
Nic M Vega
Bernardo Cervantes
Christopher P Mancuso
Ning Mao
Megan N Taylor
James J Collins
Ahmad S Khalil
Jeff Gore
Timothy K Lu
Enhancing nutritional niche and host defenses by modifying the gut microbiome
Molecular Systems Biology
bacteria community
cellulose
gut microbiome
nutrition
pathogen
title Enhancing nutritional niche and host defenses by modifying the gut microbiome
title_full Enhancing nutritional niche and host defenses by modifying the gut microbiome
title_fullStr Enhancing nutritional niche and host defenses by modifying the gut microbiome
title_full_unstemmed Enhancing nutritional niche and host defenses by modifying the gut microbiome
title_short Enhancing nutritional niche and host defenses by modifying the gut microbiome
title_sort enhancing nutritional niche and host defenses by modifying the gut microbiome
topic bacteria community
cellulose
gut microbiome
nutrition
pathogen
url https://doi.org/10.15252/msb.20209933
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