Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in China
Sweet viburnum (Viburnum odoratissimum Ker-Gawl.) is a widely used ornamental plant, which has dense branches and leaves, and fast spreading and evergreen habit. In October 2022, leaf spot symptoms were observed in a hedge of sweet viburnum in Yuanshi Garden, Ningbo, China. Fungi were isolated from...
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Firenze University Press
2024-09-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/15455 |
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| author | Wen LI Yixuan RUAN Zhengping BIAN Yueqiu HE Kai FENG Liang LIU Zhilong WANG Feng HUANG |
| author_facet | Wen LI Yixuan RUAN Zhengping BIAN Yueqiu HE Kai FENG Liang LIU Zhilong WANG Feng HUANG |
| author_sort | Wen LI |
| collection | DOAJ |
| description | Sweet viburnum (Viburnum odoratissimum Ker-Gawl.) is a widely used ornamental plant, which has dense branches and leaves, and fast spreading and evergreen habit. In October 2022, leaf spot symptoms were observed in a hedge of sweet viburnum in Yuanshi Garden, Ningbo, China. Fungi were isolated from symptomatic leaves, and were identified using morphological characteristics and phylogenetic analyses of partial sequences of internal transcribed spacer (ITS), actin (act), and translation elongation factor 1-alpha (tef1-α), and were evaluated in pathogenicity tests. The causal agent of sweet viburnum leaf spot was identified to be Phyllosticta capitalensis. Effects of seven fungicides on P. capitalensis were assessed in vitro. Fungicide EC50s (mg L-1) against P. capitalensis were: 270.77 for 75% chlorothalonil (WP); 0.02 for 250 g L-1 azoxystrobin SC; 0.27 for 10% difenconazole WDG; 0.02 for 75% trifloxystrobin + tebuconazole WDG, 9.03 for 35% fluopyram + tebuconazole SC, 5.90 for 500 g L-1 fluazinam SC, and 89.11 for 10% prothioconazole SC. Among these, azoxystrobin SC and trifloxystrobin + tebuconazole WDG could be used for control of viburnum leaf spot. This is the first report of P. capitalensis causing leaf spot of sweet viburnum, and this study provides guidance for chemical management sweet viburnum leaf spot, and on other host plants. |
| format | Article |
| id | doaj-art-dfd516a04ba04b878e456b10740f450b |
| institution | OA Journals |
| issn | 0031-9465 1593-2095 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj-art-dfd516a04ba04b878e456b10740f450b2025-08-20T02:11:29ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952024-09-0163229530110.36253/phyto-1545514292Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in ChinaWen LI0https://orcid.org/0000-0002-0983-7910Yixuan RUAN1https://orcid.org/0009-0004-1956-1833Zhengping BIAN2https://orcid.org/0009-0008-7439-175XYueqiu HE3https://orcid.org/0009-0002-8988-9012Kai FENG4https://orcid.org/0009-0000-4501-7883Liang LIU5https://orcid.org/0000-0001-8044-1006Zhilong WANG6https://orcid.org/0000-0001-8734-1729Feng HUANG7https://orcid.org/0000-0002-8406-5068Ningbo City College of Vocational Technology, Ningbo ZhejiangNingbo City College of Vocational Technology, Ningbo ZhejiangNingbo Forest Farm, Ningbo ZhejiangNingbo City College of Vocational Technology, Ningbo ZhejiangNingbo City College of Vocational Technology, Ningbo ZhejiangNingbo City College of Vocational Technology, Ningbo ZhejiangNingbo City College of Vocational Technology, Ningbo ZhejiangPlant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High, Guangzhou, GuangdongSweet viburnum (Viburnum odoratissimum Ker-Gawl.) is a widely used ornamental plant, which has dense branches and leaves, and fast spreading and evergreen habit. In October 2022, leaf spot symptoms were observed in a hedge of sweet viburnum in Yuanshi Garden, Ningbo, China. Fungi were isolated from symptomatic leaves, and were identified using morphological characteristics and phylogenetic analyses of partial sequences of internal transcribed spacer (ITS), actin (act), and translation elongation factor 1-alpha (tef1-α), and were evaluated in pathogenicity tests. The causal agent of sweet viburnum leaf spot was identified to be Phyllosticta capitalensis. Effects of seven fungicides on P. capitalensis were assessed in vitro. Fungicide EC50s (mg L-1) against P. capitalensis were: 270.77 for 75% chlorothalonil (WP); 0.02 for 250 g L-1 azoxystrobin SC; 0.27 for 10% difenconazole WDG; 0.02 for 75% trifloxystrobin + tebuconazole WDG, 9.03 for 35% fluopyram + tebuconazole SC, 5.90 for 500 g L-1 fluazinam SC, and 89.11 for 10% prothioconazole SC. Among these, azoxystrobin SC and trifloxystrobin + tebuconazole WDG could be used for control of viburnum leaf spot. This is the first report of P. capitalensis causing leaf spot of sweet viburnum, and this study provides guidance for chemical management sweet viburnum leaf spot, and on other host plants.https://oajournals.fupress.net/index.php/pm/article/view/15455 chemical controlnew diseasepathogen identificationphylogeny |
| spellingShingle | Wen LI Yixuan RUAN Zhengping BIAN Yueqiu HE Kai FENG Liang LIU Zhilong WANG Feng HUANG Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in China Phytopathologia Mediterranea chemical control new disease pathogen identification phylogeny |
| title | Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in China |
| title_full | Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in China |
| title_fullStr | Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in China |
| title_full_unstemmed | Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in China |
| title_short | Identification and fungicide screening of Phyllosticta capitalensis causing leaf spot on sweet viburnum in China |
| title_sort | identification and fungicide screening of phyllosticta capitalensis causing leaf spot on sweet viburnum in china |
| topic | chemical control new disease pathogen identification phylogeny |
| url | https://oajournals.fupress.net/index.php/pm/article/view/15455 |
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