SFRP1 suppresses granulosa cell proliferation and migration through inhibiting JNK pathway
Background: Secreted frizzled-related protein 1 (SFRP1) functions as a Wnt antagonist to repress the proliferation and migration of epithelial ovarian cancer cells. Recent research has shown that SFRP1 was reduced in the subcutaneous abdominal adipose stem cells isolated from patients with polycysti...
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IMR Press
2021-10-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/48/5/10.31083/j.ceog4805190 |
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| author | Shan Zhou Liang Xia Liyuan Han |
| author_facet | Shan Zhou Liang Xia Liyuan Han |
| author_sort | Shan Zhou |
| collection | DOAJ |
| description | Background: Secreted frizzled-related protein 1 (SFRP1) functions as a Wnt antagonist to repress the proliferation and migration of epithelial ovarian cancer cells. Recent research has shown that SFRP1 was reduced in the subcutaneous abdominal adipose stem cells isolated from patients with polycystic ovarian syndrome (PCOS). Regardless, the regulatory role and mechanism of SFRP1 in the proliferation and migration of granulosa cells during development of PCOS are scarce. Methods: SFRP1 expression was analyzed in plasma samples from patients with PCOS or immortalized human granulosa cells (KGN). Cell counting kit-8 (CCK-8) and colony formation assays were used to analyze the cell viability and proliferation of KGN, respectively. Cell apoptosis was analyzed by flow cytometry, and migration was detected by transwell. Results: SFRP1 expression was lower in plasma samples isolated from patients with PCOS than the healthy control. Immortalized human granulosa cells (KGN) also showed decreased SFRP1 expression compared to normal ovarian epithelial IOSE80 cells. pcDNA-mediated over-expression of SFRP1 reduced the cell viability and proliferation of KGN via cell counting kit-8 (CCK-8) and colony formation assays, respectively. Flow cytometry, analysis showed that the cell apoptosis of KGN was promoted by SFRP1. Ectopic expression of SFRP1 retarded cell migration with down-regulation of MMP2, MMP9, and vimentin. JNK phosphorylation was reduced in KGN with SFRP1 over-expression. Conclusion: SFRP1 contributed to the suppression of granulosa cell proliferation and migration through inhibition of JNK activation, providing a promising molecular target for PCOS. |
| format | Article |
| id | doaj-art-dfd15614cd2641b5a3c35982ed678035 |
| institution | OA Journals |
| issn | 0390-6663 |
| language | English |
| publishDate | 2021-10-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj-art-dfd15614cd2641b5a3c35982ed6780352025-08-20T02:03:42ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632021-10-014851193119910.31083/j.ceog4805190S0390-6663(21)00028-2SFRP1 suppresses granulosa cell proliferation and migration through inhibiting JNK pathwayShan Zhou0Liang Xia1Liyuan Han2Department of Endocrinology, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, 315010 Ningbo, Zhejiang, ChinaDepartment of Gynecology, Hwa Mei Hospital, University of Chinese Academy of Sciences, Ningbo Institute of Life and Health Industry, University of Chinese Academy of Sciences, 315010 Ningbo, Zhejiang, ChinaDepartment of Global Health, Ningbo Institute of Life and Health Industry, Hwa Mei Hospital, University of Chinese Academy of Sciences, 315010 Ningbo, Zhejiang, ChinaBackground: Secreted frizzled-related protein 1 (SFRP1) functions as a Wnt antagonist to repress the proliferation and migration of epithelial ovarian cancer cells. Recent research has shown that SFRP1 was reduced in the subcutaneous abdominal adipose stem cells isolated from patients with polycystic ovarian syndrome (PCOS). Regardless, the regulatory role and mechanism of SFRP1 in the proliferation and migration of granulosa cells during development of PCOS are scarce. Methods: SFRP1 expression was analyzed in plasma samples from patients with PCOS or immortalized human granulosa cells (KGN). Cell counting kit-8 (CCK-8) and colony formation assays were used to analyze the cell viability and proliferation of KGN, respectively. Cell apoptosis was analyzed by flow cytometry, and migration was detected by transwell. Results: SFRP1 expression was lower in plasma samples isolated from patients with PCOS than the healthy control. Immortalized human granulosa cells (KGN) also showed decreased SFRP1 expression compared to normal ovarian epithelial IOSE80 cells. pcDNA-mediated over-expression of SFRP1 reduced the cell viability and proliferation of KGN via cell counting kit-8 (CCK-8) and colony formation assays, respectively. Flow cytometry, analysis showed that the cell apoptosis of KGN was promoted by SFRP1. Ectopic expression of SFRP1 retarded cell migration with down-regulation of MMP2, MMP9, and vimentin. JNK phosphorylation was reduced in KGN with SFRP1 over-expression. Conclusion: SFRP1 contributed to the suppression of granulosa cell proliferation and migration through inhibition of JNK activation, providing a promising molecular target for PCOS.https://www.imrpress.com/journal/CEOG/48/5/10.31083/j.ceog4805190sfrp1granulosa cellproliferationmigrationjnkpolycystic ovarian syndrome |
| spellingShingle | Shan Zhou Liang Xia Liyuan Han SFRP1 suppresses granulosa cell proliferation and migration through inhibiting JNK pathway Clinical and Experimental Obstetrics & Gynecology sfrp1 granulosa cell proliferation migration jnk polycystic ovarian syndrome |
| title | SFRP1 suppresses granulosa cell proliferation and migration through inhibiting JNK pathway |
| title_full | SFRP1 suppresses granulosa cell proliferation and migration through inhibiting JNK pathway |
| title_fullStr | SFRP1 suppresses granulosa cell proliferation and migration through inhibiting JNK pathway |
| title_full_unstemmed | SFRP1 suppresses granulosa cell proliferation and migration through inhibiting JNK pathway |
| title_short | SFRP1 suppresses granulosa cell proliferation and migration through inhibiting JNK pathway |
| title_sort | sfrp1 suppresses granulosa cell proliferation and migration through inhibiting jnk pathway |
| topic | sfrp1 granulosa cell proliferation migration jnk polycystic ovarian syndrome |
| url | https://www.imrpress.com/journal/CEOG/48/5/10.31083/j.ceog4805190 |
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