Induction of Hsp104 by Cr(VI) in yeast Candida intermedia
The synthesis of a polypeptide family named heat shock or stress proteins is one of the most conserved mechanisms of cellular protection in the case of changes in the environment. Among them Hsp104 is important, because it promotes survival of cells under extreme stresses such as high temperatures,...
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| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2005-05-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/15260 |
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| author | Polona JAMNIK Peter RASPOR |
| author_facet | Polona JAMNIK Peter RASPOR |
| author_sort | Polona JAMNIK |
| collection | DOAJ |
| description | The synthesis of a polypeptide family named heat shock or stress proteins is one of the most conserved mechanisms of cellular protection in the case of changes in the environment. Among them Hsp104 is important, because it promotes survival of cells under extreme stresses such as high temperatures, severe oxidative damage and high concentrations of ethanol. The aim of our research was to investigate whether Hsp104 is induced in the yeast Candida intermedia exposed to Cr(VI). Namely, Cr(VI) belongs to redox active metals, which play an important role in the generation of reactive oxygen species in the cell and therefore they can lead to oxidative damages. Yeast cells were treated with Cr(VI) in the mid-exponential phase and after a defined time of incubation, Hsp104 induction was investigated by using Western blotting. Results showed that cultivation of yeast cells in the presence of 100 mM Cr(VI) caused induction of Hsp104, which is connected to formation of protein aggregates. Hsp104 assists their resolubilization and so contributes to cell survival. Its synthesis was detected 0.5 h after Cr(VI) addition and was later for up to 2 h more apparent. Therefore, we showed that Hsp104 plays an important role in the stress response of yeast Candida intermedia to Cr(VI). |
| format | Article |
| id | doaj-art-dfbc02e086e24431a7ded54ebeb94a68 |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2005-05-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-dfbc02e086e24431a7ded54ebeb94a682025-08-20T03:02:26ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412005-05-01851677210.14720/aas.2005.85.1.1526021653Induction of Hsp104 by Cr(VI) in yeast Candida intermediaPolona JAMNIK0Peter RASPOR1University of Ljubljana, Biotechnical faculty, Food Science and Technology Department, Chair of Biotechnology, Jamnikarjeva 101, 1000 Ljubljana, SloveniaUniversity of Ljubljana, Biotechnical faculty, Food Science and Technology Department, Chair of Biotechnology, Jamnikarjeva 101, 1000 Ljubljana, SloveniaThe synthesis of a polypeptide family named heat shock or stress proteins is one of the most conserved mechanisms of cellular protection in the case of changes in the environment. Among them Hsp104 is important, because it promotes survival of cells under extreme stresses such as high temperatures, severe oxidative damage and high concentrations of ethanol. The aim of our research was to investigate whether Hsp104 is induced in the yeast Candida intermedia exposed to Cr(VI). Namely, Cr(VI) belongs to redox active metals, which play an important role in the generation of reactive oxygen species in the cell and therefore they can lead to oxidative damages. Yeast cells were treated with Cr(VI) in the mid-exponential phase and after a defined time of incubation, Hsp104 induction was investigated by using Western blotting. Results showed that cultivation of yeast cells in the presence of 100 mM Cr(VI) caused induction of Hsp104, which is connected to formation of protein aggregates. Hsp104 assists their resolubilization and so contributes to cell survival. Its synthesis was detected 0.5 h after Cr(VI) addition and was later for up to 2 h more apparent. Therefore, we showed that Hsp104 plays an important role in the stress response of yeast Candida intermedia to Cr(VI).https://journals.uni-lj.si/aas/article/view/15260yeastscandida intermediachromiumoxidative stressstress proteinshsp104 |
| spellingShingle | Polona JAMNIK Peter RASPOR Induction of Hsp104 by Cr(VI) in yeast Candida intermedia Acta Agriculturae Slovenica yeasts candida intermedia chromium oxidative stress stress proteins hsp104 |
| title | Induction of Hsp104 by Cr(VI) in yeast Candida intermedia |
| title_full | Induction of Hsp104 by Cr(VI) in yeast Candida intermedia |
| title_fullStr | Induction of Hsp104 by Cr(VI) in yeast Candida intermedia |
| title_full_unstemmed | Induction of Hsp104 by Cr(VI) in yeast Candida intermedia |
| title_short | Induction of Hsp104 by Cr(VI) in yeast Candida intermedia |
| title_sort | induction of hsp104 by cr vi in yeast candida intermedia |
| topic | yeasts candida intermedia chromium oxidative stress stress proteins hsp104 |
| url | https://journals.uni-lj.si/aas/article/view/15260 |
| work_keys_str_mv | AT polonajamnik inductionofhsp104bycrviinyeastcandidaintermedia AT peterraspor inductionofhsp104bycrviinyeastcandidaintermedia |