The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyes
Vernal keratoconjunctivitis (VKC) is a chronic allergic ocular surface disease with seasonal recurrences and severe forms showing vision threatening complications. The purpose of the study is to understand the prevalence and diversity of biofilm-forming bacteria and antimicrobial resistance in VKC c...
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Elsevier
2025-06-01
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| author | Nelaveni Rupa Pragnya Rao Donthineni Sayan Basu Kotakonda Arunasri |
| author_facet | Nelaveni Rupa Pragnya Rao Donthineni Sayan Basu Kotakonda Arunasri |
| author_sort | Nelaveni Rupa |
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| description | Vernal keratoconjunctivitis (VKC) is a chronic allergic ocular surface disease with seasonal recurrences and severe forms showing vision threatening complications. The purpose of the study is to understand the prevalence and diversity of biofilm-forming bacteria and antimicrobial resistance in VKC compared to healthy individuals (HC). For this, conjunctival swab samples were collected from VKC (n = 26) and HC (n = 23), of which culture positive samples were 77 % and 78.26 % respectively. The 16S rRNA gene sequencing revealed a significant increase in bacterial diversity in VKC compared to HC (p < 0.05), identifying 16 and 9 bacterial species, respectively. Staphylococcus epidermidis emerged as the predominant bacterium in both groups, with relative abundances of 52.8 % in HC and 30.2 % in VKC (p < 0.001). Biofilm formation was observed in 64.15 % of bacterial species in VKC and 31 % in HC (p < 0.001). Scanning electron microscopy analysis confirmed temporal biofilm formation by Staphylococcus spp. in both groups. Minimum inhibitory concentration testing showed that biofilm forming Staphylococcus spp. from VKC exhibited multidrug resistance (>2 antibiotics) more frequently than those from HC. Additionally, Staphylococcus spp. in VKC demonstrated higher resistance to fluoroquinolones compared to HC. These findings indicate a significantly greater prevalence of biofilm-forming and antimicrobial resistant Staphylococcus bacteria in VKC Patients compared with HC. |
| format | Article |
| id | doaj-art-dfbae0cd318340d5a39384b22d03a8fd |
| institution | OA Journals |
| issn | 2590-2075 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Biofilm |
| spelling | doaj-art-dfbae0cd318340d5a39384b22d03a8fd2025-08-20T02:16:02ZengElsevierBiofilm2590-20752025-06-01910027810.1016/j.bioflm.2025.100278The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyesNelaveni Rupa0Pragnya Rao Donthineni1Sayan Basu2Kotakonda Arunasri3Prof. Brien Holden Eye Research Centre, L V Prasad Eye Institute, Banjara Hills, Hyderabad, 500034, Telangana, IndiaShantilal Shanghvi Cornea Institute, L V Prasad Eye Institute, Hyderabad, 500034, Telangana, IndiaProf. Brien Holden Eye Research Centre, L V Prasad Eye Institute, Banjara Hills, Hyderabad, 500034, Telangana, India; Shantilal Shanghvi Cornea Institute, L V Prasad Eye Institute, Hyderabad, 500034, Telangana, IndiaProf. Brien Holden Eye Research Centre, L V Prasad Eye Institute, Banjara Hills, Hyderabad, 500034, Telangana, India; Corresponding author. Brien Holden Eye Research Centre, L V Prasad Eye Institute, Banjara Hills, Hyderabad, 500034, Telangana, India.Vernal keratoconjunctivitis (VKC) is a chronic allergic ocular surface disease with seasonal recurrences and severe forms showing vision threatening complications. The purpose of the study is to understand the prevalence and diversity of biofilm-forming bacteria and antimicrobial resistance in VKC compared to healthy individuals (HC). For this, conjunctival swab samples were collected from VKC (n = 26) and HC (n = 23), of which culture positive samples were 77 % and 78.26 % respectively. The 16S rRNA gene sequencing revealed a significant increase in bacterial diversity in VKC compared to HC (p < 0.05), identifying 16 and 9 bacterial species, respectively. Staphylococcus epidermidis emerged as the predominant bacterium in both groups, with relative abundances of 52.8 % in HC and 30.2 % in VKC (p < 0.001). Biofilm formation was observed in 64.15 % of bacterial species in VKC and 31 % in HC (p < 0.001). Scanning electron microscopy analysis confirmed temporal biofilm formation by Staphylococcus spp. in both groups. Minimum inhibitory concentration testing showed that biofilm forming Staphylococcus spp. from VKC exhibited multidrug resistance (>2 antibiotics) more frequently than those from HC. Additionally, Staphylococcus spp. in VKC demonstrated higher resistance to fluoroquinolones compared to HC. These findings indicate a significantly greater prevalence of biofilm-forming and antimicrobial resistant Staphylococcus bacteria in VKC Patients compared with HC.http://www.sciencedirect.com/science/article/pii/S2590207525000267Staphylococcus biofilmsAntimicrobial resistanceVernal keratoconjunctivitisConjunctival bacteria |
| spellingShingle | Nelaveni Rupa Pragnya Rao Donthineni Sayan Basu Kotakonda Arunasri The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyes Biofilm Staphylococcus biofilms Antimicrobial resistance Vernal keratoconjunctivitis Conjunctival bacteria |
| title | The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyes |
| title_full | The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyes |
| title_fullStr | The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyes |
| title_full_unstemmed | The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyes |
| title_short | The burden of antimicrobial resistance in biofilm forming Staphylococcus spp. from Vernal Keratoconjunctivitis patients eyes |
| title_sort | burden of antimicrobial resistance in biofilm forming staphylococcus spp from vernal keratoconjunctivitis patients eyes |
| topic | Staphylococcus biofilms Antimicrobial resistance Vernal keratoconjunctivitis Conjunctival bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S2590207525000267 |
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