Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats
Background: Cardiac aging is associated with myocardial remodeling and reduced angiogenesis. Counteracting these changes with natural products is a preventive strategy with great potential. The aim of this study was to evaluate the effect of <i>Aronia melanocarpa</i> fruit juice (AMJ) su...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/15/1/23 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588166874267648 |
|---|---|
| author | Elena Daskalova Mina Pencheva Slavi Delchev Lyudmila Vladimirova-Kitova Spas Kitov Stoyan Markov David Baruh Petko Denev |
| author_facet | Elena Daskalova Mina Pencheva Slavi Delchev Lyudmila Vladimirova-Kitova Spas Kitov Stoyan Markov David Baruh Petko Denev |
| author_sort | Elena Daskalova |
| collection | DOAJ |
| description | Background: Cardiac aging is associated with myocardial remodeling and reduced angiogenesis. Counteracting these changes with natural products is a preventive strategy with great potential. The aim of this study was to evaluate the effect of <i>Aronia melanocarpa</i> fruit juice (AMJ) supplementation on age-related myocardial remodeling in aged rat hearts. Methods: Healthy male Wistar rats (n = 24) were divided into three groups: (1) young controls (CY)—age 2 months; (2) old controls (CO)—age 27 months; (3) AMJ group—27-month-old animals, supplemented with <i>Aronia melanocarpa</i> juice (AMJ) at a dose of 10 mL∙kg<sup>−1</sup> for 105 days. After this period, the hearts of the animals were fixed, embedded in paraffin, and immunohistochemical and morphometric analyses were performed. Results: A higher vascular and capillary density was found in the hearts of the AMJ group, as compared to CO. The mean number of CD34+ cells in the myocardium increased by 18.6% in the AMJ group, as compared to CO (<i>p</i> < 0.05). Furthermore, the angiotensin converting enzyme 2 (ACE2) immunoexpression in the myocardium increased by 37% (<i>p</i> < 0.05) and the Proto-oncogene Mas receptor (MAS1) immunoexpression increased by 6% (<i>p</i> < 0.05) in the AMJ group, as compared to CO. Conclusions: As a result of the application of AMJ, noticeable neovascularization was found, which indicates improved myocardial nourishment. The present study demonstrates for the first time that polyphenol-rich AMJ can positively influence age-related microvascular myocardial remodeling in rats, thus outlining its potential as a preventive agent for healthy cardiac aging. |
| format | Article |
| id | doaj-art-dfaf3cf270b140aa8559685029a5e85d |
| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-dfaf3cf270b140aa8559685029a5e85d2025-01-24T13:38:29ZengMDPI AGLife2075-17292024-12-011512310.3390/life15010023Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in RatsElena Daskalova0Mina Pencheva1Slavi Delchev2Lyudmila Vladimirova-Kitova3Spas Kitov4Stoyan Markov5David Baruh6Petko Denev7Department of Anatomy, Histology and Embryology, Medical Faculty, Medical University, 4000 Plovdiv, BulgariaDepartment of Medical Physics and Biophysics, Faculty of Pharmacy, Medical University, 4000 Plovdiv, BulgariaDepartment of Anatomy, Histology and Embryology, Medical Faculty, Medical University, 4000 Plovdiv, BulgariaI-st Department of Internal Diseases, Cardiology Section Medical Faculty, Medical University, 4000 Plovdiv, BulgariaI-st Department of Internal Diseases, Cardiology Section Medical Faculty, Medical University, 4000 Plovdiv, BulgariaDepartment of Otorhinolaryngology, Medical Faculty, Medical University, 4000 Plovdiv, BulgariaDepartment of Software Engineering, Faculty of Mathematics and Informatics, Sofia University “St. Kliment Ohridski”, 1504 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Laboratory of Biologically Active Substances, 4000 Plovdiv, BulgariaBackground: Cardiac aging is associated with myocardial remodeling and reduced angiogenesis. Counteracting these changes with natural products is a preventive strategy with great potential. The aim of this study was to evaluate the effect of <i>Aronia melanocarpa</i> fruit juice (AMJ) supplementation on age-related myocardial remodeling in aged rat hearts. Methods: Healthy male Wistar rats (n = 24) were divided into three groups: (1) young controls (CY)—age 2 months; (2) old controls (CO)—age 27 months; (3) AMJ group—27-month-old animals, supplemented with <i>Aronia melanocarpa</i> juice (AMJ) at a dose of 10 mL∙kg<sup>−1</sup> for 105 days. After this period, the hearts of the animals were fixed, embedded in paraffin, and immunohistochemical and morphometric analyses were performed. Results: A higher vascular and capillary density was found in the hearts of the AMJ group, as compared to CO. The mean number of CD34+ cells in the myocardium increased by 18.6% in the AMJ group, as compared to CO (<i>p</i> < 0.05). Furthermore, the angiotensin converting enzyme 2 (ACE2) immunoexpression in the myocardium increased by 37% (<i>p</i> < 0.05) and the Proto-oncogene Mas receptor (MAS1) immunoexpression increased by 6% (<i>p</i> < 0.05) in the AMJ group, as compared to CO. Conclusions: As a result of the application of AMJ, noticeable neovascularization was found, which indicates improved myocardial nourishment. The present study demonstrates for the first time that polyphenol-rich AMJ can positively influence age-related microvascular myocardial remodeling in rats, thus outlining its potential as a preventive agent for healthy cardiac aging.https://www.mdpi.com/2075-1729/15/1/23black chokeberry (<i>Aronia melanocarpa</i>)ageing heartneovascularizationACE2CD34 |
| spellingShingle | Elena Daskalova Mina Pencheva Slavi Delchev Lyudmila Vladimirova-Kitova Spas Kitov Stoyan Markov David Baruh Petko Denev Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats Life black chokeberry (<i>Aronia melanocarpa</i>) ageing heart neovascularization ACE2 CD34 |
| title | Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats |
| title_full | Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats |
| title_fullStr | Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats |
| title_full_unstemmed | Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats |
| title_short | Black Chokeberry (<i>Aronia melanocarpa</i>) Juice Supplementation Affects Age-Related Myocardial Remodeling in Rats |
| title_sort | black chokeberry i aronia melanocarpa i juice supplementation affects age related myocardial remodeling in rats |
| topic | black chokeberry (<i>Aronia melanocarpa</i>) ageing heart neovascularization ACE2 CD34 |
| url | https://www.mdpi.com/2075-1729/15/1/23 |
| work_keys_str_mv | AT elenadaskalova blackchokeberryiaroniamelanocarpaijuicesupplementationaffectsagerelatedmyocardialremodelinginrats AT minapencheva blackchokeberryiaroniamelanocarpaijuicesupplementationaffectsagerelatedmyocardialremodelinginrats AT slavidelchev blackchokeberryiaroniamelanocarpaijuicesupplementationaffectsagerelatedmyocardialremodelinginrats AT lyudmilavladimirovakitova blackchokeberryiaroniamelanocarpaijuicesupplementationaffectsagerelatedmyocardialremodelinginrats AT spaskitov blackchokeberryiaroniamelanocarpaijuicesupplementationaffectsagerelatedmyocardialremodelinginrats AT stoyanmarkov blackchokeberryiaroniamelanocarpaijuicesupplementationaffectsagerelatedmyocardialremodelinginrats AT davidbaruh blackchokeberryiaroniamelanocarpaijuicesupplementationaffectsagerelatedmyocardialremodelinginrats AT petkodenev blackchokeberryiaroniamelanocarpaijuicesupplementationaffectsagerelatedmyocardialremodelinginrats |