An integrated methodological framework for the validation and verification of clinical testing by qRT-PCR

An integrated methodological framework for the validation and verification of clinical testing by qRT-PCR

This paper outlines a practical method for validating quantitative-qualitative techniques used to detect genetic material through qRT-PCR, specifically focusing on SARS-CoV-2 testing and adhering to ISO/IEC 17025:2018 accreditation standards. Despite the prevalence of quantitative-qualitative screen...

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Bibliographic Details
Main Authors: Carolina Cardona-Ramírez, Cruz Elena Enríquez-Valencia, Gina Méndez-Callejas, Giovanna Meza Barreto, Gabriel Andrés Tafur-Gómez, Danny Wilson Sanjuanelo-Corredor
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Heliyon
Subjects:
Accuracy
Reliability
Precision
Specificity, Sensitivity
qRT-PCR
ISO/IEC 17025
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024171198
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