Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul
Colistin is considered the last resort for treating infections caused by multidrug-resistant bacteria. However, the spread of the plasmid-borne colistin-resistance gene mcr-1 has become a public health threat. In this study, we identified mcr-1-harboring Leclercia adecarboxylata strain (WWCOL-134) i...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524001565 |
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| author | Jin Seok Kim Hyo-Won Jeong Young Hee Jin JinWoo Kim Jin-ah Kim Sook Hyun Park So-Mi Yoon Sang-Eun Jung Jung Im Jang Eun Ji Kim Jae In Lee Jib-Ho Lee |
| author_facet | Jin Seok Kim Hyo-Won Jeong Young Hee Jin JinWoo Kim Jin-ah Kim Sook Hyun Park So-Mi Yoon Sang-Eun Jung Jung Im Jang Eun Ji Kim Jae In Lee Jib-Ho Lee |
| author_sort | Jin Seok Kim |
| collection | DOAJ |
| description | Colistin is considered the last resort for treating infections caused by multidrug-resistant bacteria. However, the spread of the plasmid-borne colistin-resistance gene mcr-1 has become a public health threat. In this study, we identified mcr-1-harboring Leclercia adecarboxylata strain (WWCOL-134) isolated from wastewater in Seoul. The strain had a colistin MIC value of 2 µg/ml and was resistant to cefotaxime, gentamicin, tetracycline, trimethoprim and sulfamethoxazole. The mcr-1 gene, along with an array of resistance genes, was located on a 236-kb plasmid (pCOL134-1), which contained the typical IncHI2 backbone of reported mcr-1-carrying plasmids, and was transferred to an Escherichia coli strain by conjugation. To the best of our knowledge, this is the first study to report the emergence of mcr-1-harboring Leclercia sp. isolate. Our findings demonstrate the ongoing spread of colistin resistance among Enterobacterales species, emphasizing the need for surveillance of antimicrobial resistance in wastewater environments. |
| format | Article |
| id | doaj-art-dfa9bfa9a15741e2a9c0f5e9d5064bac |
| institution | OA Journals |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-dfa9bfa9a15741e2a9c0f5e9d5064bac2025-08-20T02:35:03ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-0139374010.1016/j.jgar.2024.08.003Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in SeoulJin Seok Kim0Hyo-Won Jeong1Young Hee Jin2JinWoo Kim3Jin-ah Kim4Sook Hyun Park5So-Mi Yoon6Sang-Eun Jung7Jung Im Jang8Eun Ji Kim9Jae In Lee10Jib-Ho Lee11Corresponding author: Emerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, 202-3, Yangjae-dong, Seocho-gu, Seoul, 13818, Republic of Korea.; Emerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaEmerging Infectious Diseases Team, Seoul Metropolitan Government Research Institute of Public Health and Environment, Seoul, Republic of KoreaColistin is considered the last resort for treating infections caused by multidrug-resistant bacteria. However, the spread of the plasmid-borne colistin-resistance gene mcr-1 has become a public health threat. In this study, we identified mcr-1-harboring Leclercia adecarboxylata strain (WWCOL-134) isolated from wastewater in Seoul. The strain had a colistin MIC value of 2 µg/ml and was resistant to cefotaxime, gentamicin, tetracycline, trimethoprim and sulfamethoxazole. The mcr-1 gene, along with an array of resistance genes, was located on a 236-kb plasmid (pCOL134-1), which contained the typical IncHI2 backbone of reported mcr-1-carrying plasmids, and was transferred to an Escherichia coli strain by conjugation. To the best of our knowledge, this is the first study to report the emergence of mcr-1-harboring Leclercia sp. isolate. Our findings demonstrate the ongoing spread of colistin resistance among Enterobacterales species, emphasizing the need for surveillance of antimicrobial resistance in wastewater environments.http://www.sciencedirect.com/science/article/pii/S2213716524001565Leclercia adecarboxylataColistinmcr-1IncHI2Wastewater |
| spellingShingle | Jin Seok Kim Hyo-Won Jeong Young Hee Jin JinWoo Kim Jin-ah Kim Sook Hyun Park So-Mi Yoon Sang-Eun Jung Jung Im Jang Eun Ji Kim Jae In Lee Jib-Ho Lee Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul Journal of Global Antimicrobial Resistance Leclercia adecarboxylata Colistin mcr-1 IncHI2 Wastewater |
| title | Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul |
| title_full | Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul |
| title_fullStr | Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul |
| title_full_unstemmed | Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul |
| title_short | Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul |
| title_sort | emergence of the mobile colistin resistance gene mcr 1 in a leclercia adecarboxylata strain isolated from wastewater in seoul |
| topic | Leclercia adecarboxylata Colistin mcr-1 IncHI2 Wastewater |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524001565 |
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