Karyotype uniformity in populations of the endemic Hemiancistrus fuliginosus (Loricariidae: Hypostomini) collected in the upper and middle Uruguai River
Abstract Disagreements in molecular and morphological analyzes have generated conflicts about the correct allocation of Hemiancistrus fuliginosus in Hypostominae. In this study, cytogenetics analyzes in four populations of H. fuliginosus from tributaries of the Uruguai Riverrevealed 2n = 56 chromoso...
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Sociedade Brasileira de Ictiologia
2025-02-01
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| Series: | Neotropical Ichthyology |
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| author | Jocicléia Thums Konerat Vanessa Bueno Ana Luiza de Brito Portela-Castro Isabel Cristina Martins-Santos Vladimir Pavan Margarido |
| author_facet | Jocicléia Thums Konerat Vanessa Bueno Ana Luiza de Brito Portela-Castro Isabel Cristina Martins-Santos Vladimir Pavan Margarido |
| author_sort | Jocicléia Thums Konerat |
| collection | DOAJ |
| description | Abstract Disagreements in molecular and morphological analyzes have generated conflicts about the correct allocation of Hemiancistrus fuliginosus in Hypostominae. In this study, cytogenetics analyzes in four populations of H. fuliginosus from tributaries of the Uruguai Riverrevealed 2n = 56 chromosomes (30m + 18sm + 6st + 2a) for all populations. Nucleolar organizer regions (NORs) were located on the short arm in terminal position of the subtelocentric chromosome pair 25 in all population, in addition to Antas River population showed a structural polymorphism (three different phenotypes). Physical mapping of 5S rDNA showed cistrons in pericentromeric position on the short arm of the metacentric chromosome pair 12 in all the populations. Centromeric heterochromatins are present in almost all chromosomes, and conspicuous CMA3+/DAPI- blocks coincident with rDNA sites. Chromosomal data were important markers to fill gaps and to contribute to morphological and molecular proposals in allocating H. fuliginosus. The exclusivity of NORs polymorphism of the Antas River population can be attributed to the geomorphological characteristics of the tributary that restrict gene flow, while karyotypic similarities among the other three populations would be provided by the species’ ability to disperse. |
| format | Article |
| id | doaj-art-dfa99fe0510941be8dc14affd6020a6f |
| institution | Kabale University |
| issn | 1982-0224 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Sociedade Brasileira de Ictiologia |
| record_format | Article |
| series | Neotropical Ichthyology |
| spelling | doaj-art-dfa99fe0510941be8dc14affd6020a6f2025-02-11T07:42:55ZengSociedade Brasileira de IctiologiaNeotropical Ichthyology1982-02242025-02-0123110.1590/1982-0224-2024-0058Karyotype uniformity in populations of the endemic Hemiancistrus fuliginosus (Loricariidae: Hypostomini) collected in the upper and middle Uruguai RiverJocicléia Thums Konerathttps://orcid.org/0000-0002-4092-6532Vanessa Buenohttps://orcid.org/0000-0003-0508-1666Ana Luiza de Brito Portela-Castrohttps://orcid.org/0000-0002-0881-0345Isabel Cristina Martins-Santoshttps://orcid.org/0000-0003-4814-5607Vladimir Pavan Margaridohttps://orcid.org/0000-0002-0823-6646Abstract Disagreements in molecular and morphological analyzes have generated conflicts about the correct allocation of Hemiancistrus fuliginosus in Hypostominae. In this study, cytogenetics analyzes in four populations of H. fuliginosus from tributaries of the Uruguai Riverrevealed 2n = 56 chromosomes (30m + 18sm + 6st + 2a) for all populations. Nucleolar organizer regions (NORs) were located on the short arm in terminal position of the subtelocentric chromosome pair 25 in all population, in addition to Antas River population showed a structural polymorphism (three different phenotypes). Physical mapping of 5S rDNA showed cistrons in pericentromeric position on the short arm of the metacentric chromosome pair 12 in all the populations. Centromeric heterochromatins are present in almost all chromosomes, and conspicuous CMA3+/DAPI- blocks coincident with rDNA sites. Chromosomal data were important markers to fill gaps and to contribute to morphological and molecular proposals in allocating H. fuliginosus. The exclusivity of NORs polymorphism of the Antas River population can be attributed to the geomorphological characteristics of the tributary that restrict gene flow, while karyotypic similarities among the other three populations would be provided by the species’ ability to disperse.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252025000100201&lng=en&tlng=enChromosomal evolutionKaryotypic similaritiesParacentric inversionrDNA-FISH |
| spellingShingle | Jocicléia Thums Konerat Vanessa Bueno Ana Luiza de Brito Portela-Castro Isabel Cristina Martins-Santos Vladimir Pavan Margarido Karyotype uniformity in populations of the endemic Hemiancistrus fuliginosus (Loricariidae: Hypostomini) collected in the upper and middle Uruguai River Neotropical Ichthyology Chromosomal evolution Karyotypic similarities Paracentric inversion rDNA-FISH |
| title | Karyotype uniformity in populations of the endemic Hemiancistrus fuliginosus (Loricariidae: Hypostomini) collected in the upper and middle Uruguai River |
| title_full | Karyotype uniformity in populations of the endemic Hemiancistrus fuliginosus (Loricariidae: Hypostomini) collected in the upper and middle Uruguai River |
| title_fullStr | Karyotype uniformity in populations of the endemic Hemiancistrus fuliginosus (Loricariidae: Hypostomini) collected in the upper and middle Uruguai River |
| title_full_unstemmed | Karyotype uniformity in populations of the endemic Hemiancistrus fuliginosus (Loricariidae: Hypostomini) collected in the upper and middle Uruguai River |
| title_short | Karyotype uniformity in populations of the endemic Hemiancistrus fuliginosus (Loricariidae: Hypostomini) collected in the upper and middle Uruguai River |
| title_sort | karyotype uniformity in populations of the endemic hemiancistrus fuliginosus loricariidae hypostomini collected in the upper and middle uruguai river |
| topic | Chromosomal evolution Karyotypic similarities Paracentric inversion rDNA-FISH |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252025000100201&lng=en&tlng=en |
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