Micro-CT evaluation of dentinal microcrack formation in mesiobuccal canals of maxillary molars following instrumentation with heat-treated rotary and reciprocating systems

Abstract Background The goal of root canal preparation is to eliminate microorganisms and debris from the root canal system, while providing space for the placement of root filling materials. However, the process can lead to the formation of dentin microcracks, increasing the risk of vertical root f...

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Main Authors: Fatemeh Soltaninejad, Yazdan Shantiaee, Babak Zandi, Arsham Moslemi, Seyed Sepehr Mirebeigi-Jamasbi
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Oral Health
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Online Access:https://doi.org/10.1186/s12903-025-05929-z
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author Fatemeh Soltaninejad
Yazdan Shantiaee
Babak Zandi
Arsham Moslemi
Seyed Sepehr Mirebeigi-Jamasbi
author_facet Fatemeh Soltaninejad
Yazdan Shantiaee
Babak Zandi
Arsham Moslemi
Seyed Sepehr Mirebeigi-Jamasbi
author_sort Fatemeh Soltaninejad
collection DOAJ
description Abstract Background The goal of root canal preparation is to eliminate microorganisms and debris from the root canal system, while providing space for the placement of root filling materials. However, the process can lead to the formation of dentin microcracks, increasing the risk of vertical root fractures. This study used micro-CT to evaluate the formation of dentinal microcracks following instrumentation with One Curve, EdgeOne Fire, and VDW.ROTATE systems. Methods Thirty-three extracted maxillary molars with non-calcified Vertucci type 4 s mesiobuccal canals were included. After pre-instrumentation micro-CT scans, a single endodontist prepared the canals with three aforementioned rotary systems. Post-instrumentation scans were conducted and axial sections from both the pre- and post-preparation scans were analyzed by two blinded observers to identify newly formed microcracks. Cohen’s Kappa coefficient assessed interobserver agreement and dentinal microcracks were evaluated using ANOVA, ANCOVA, Kruskal-Wallis, and McNemar’s test (P < 0.05). Results All three rotary systems led to significant increases in dentinal microcracks post-instrumentation. While the VDW.ROTATE group showed a slightly higher percentage of cracked Sect. (72%), statistical analysis showed no significant difference between the groups (P = 0.35). Conclusion Preparation of maxillary molar root canals using different rotary systems resulted in the formation of dentin microcracks, with no significant correlation to the specific system used.
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spelling doaj-art-dfa3510ce44b4c65be081bd2d77f91212025-08-20T02:12:03ZengBMCBMC Oral Health1472-68312025-04-012511810.1186/s12903-025-05929-zMicro-CT evaluation of dentinal microcrack formation in mesiobuccal canals of maxillary molars following instrumentation with heat-treated rotary and reciprocating systemsFatemeh Soltaninejad0Yazdan Shantiaee1Babak Zandi2Arsham Moslemi3Seyed Sepehr Mirebeigi-Jamasbi4Department of Endodontics, School of Dentistry, Shahid Beheshti University of Medical SciencesDepartment of Endodontics, School of Dentistry, Shahid Beheshti University of Medical SciencesDepartment of Endodontics, School of Dentistry, Shahid Beheshti University of Medical SciencesResearch Committee, School of Dentistry, Shahid Beheshti University of Medical SciencesResearch Committee, School of Dentistry, Shahid Beheshti University of Medical SciencesAbstract Background The goal of root canal preparation is to eliminate microorganisms and debris from the root canal system, while providing space for the placement of root filling materials. However, the process can lead to the formation of dentin microcracks, increasing the risk of vertical root fractures. This study used micro-CT to evaluate the formation of dentinal microcracks following instrumentation with One Curve, EdgeOne Fire, and VDW.ROTATE systems. Methods Thirty-three extracted maxillary molars with non-calcified Vertucci type 4 s mesiobuccal canals were included. After pre-instrumentation micro-CT scans, a single endodontist prepared the canals with three aforementioned rotary systems. Post-instrumentation scans were conducted and axial sections from both the pre- and post-preparation scans were analyzed by two blinded observers to identify newly formed microcracks. Cohen’s Kappa coefficient assessed interobserver agreement and dentinal microcracks were evaluated using ANOVA, ANCOVA, Kruskal-Wallis, and McNemar’s test (P < 0.05). Results All three rotary systems led to significant increases in dentinal microcracks post-instrumentation. While the VDW.ROTATE group showed a slightly higher percentage of cracked Sect. (72%), statistical analysis showed no significant difference between the groups (P = 0.35). Conclusion Preparation of maxillary molar root canals using different rotary systems resulted in the formation of dentin microcracks, with no significant correlation to the specific system used.https://doi.org/10.1186/s12903-025-05929-zDentinal microcrackMicro-CTRoot Canal PreparationRotary file systemsReciprocating file systems
spellingShingle Fatemeh Soltaninejad
Yazdan Shantiaee
Babak Zandi
Arsham Moslemi
Seyed Sepehr Mirebeigi-Jamasbi
Micro-CT evaluation of dentinal microcrack formation in mesiobuccal canals of maxillary molars following instrumentation with heat-treated rotary and reciprocating systems
BMC Oral Health
Dentinal microcrack
Micro-CT
Root Canal Preparation
Rotary file systems
Reciprocating file systems
title Micro-CT evaluation of dentinal microcrack formation in mesiobuccal canals of maxillary molars following instrumentation with heat-treated rotary and reciprocating systems
title_full Micro-CT evaluation of dentinal microcrack formation in mesiobuccal canals of maxillary molars following instrumentation with heat-treated rotary and reciprocating systems
title_fullStr Micro-CT evaluation of dentinal microcrack formation in mesiobuccal canals of maxillary molars following instrumentation with heat-treated rotary and reciprocating systems
title_full_unstemmed Micro-CT evaluation of dentinal microcrack formation in mesiobuccal canals of maxillary molars following instrumentation with heat-treated rotary and reciprocating systems
title_short Micro-CT evaluation of dentinal microcrack formation in mesiobuccal canals of maxillary molars following instrumentation with heat-treated rotary and reciprocating systems
title_sort micro ct evaluation of dentinal microcrack formation in mesiobuccal canals of maxillary molars following instrumentation with heat treated rotary and reciprocating systems
topic Dentinal microcrack
Micro-CT
Root Canal Preparation
Rotary file systems
Reciprocating file systems
url https://doi.org/10.1186/s12903-025-05929-z
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