Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants

This review provides a comprehensive summary of the latest international research on detection methods for glucosinolates in cruciferous plants. This article examines various analytical techniques, including high-performance liquid chromatography (HPLC), liquid chromatography–mass spectrometry (LC-M...

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Main Authors: Xuaner Li, Dongna Wen, Yafei He, Yumei Liu, Fengqing Han, Jialin Su, Shangxiang Lai, Mu Zhuang, Fuxing Gao, Zhansheng Li
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/13/24/4141
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author Xuaner Li
Dongna Wen
Yafei He
Yumei Liu
Fengqing Han
Jialin Su
Shangxiang Lai
Mu Zhuang
Fuxing Gao
Zhansheng Li
author_facet Xuaner Li
Dongna Wen
Yafei He
Yumei Liu
Fengqing Han
Jialin Su
Shangxiang Lai
Mu Zhuang
Fuxing Gao
Zhansheng Li
author_sort Xuaner Li
collection DOAJ
description This review provides a comprehensive summary of the latest international research on detection methods for glucosinolates in cruciferous plants. This article examines various analytical techniques, including high-performance liquid chromatography (HPLC), liquid chromatography–mass spectrometry (LC-MS), enzyme-linked immunosorbent assay (ELISA), and capillary electrophoresis (CE), while highlighting their respective advantages and limitations. Additionally, this review delves into recent advancements in sample preparation, extraction, and quantification methods, offering valuable insights into the accurate and efficient determination of glucosinolate content across diverse plant materials. Furthermore, it underscores the critical importance of the standardization and validation of these methodologies to ensure reliable glucosinolate analyses in both scientific research and industrial applications.
format Article
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spelling doaj-art-dfa0af72be9241ada7c74db2863797e82025-08-20T02:50:59ZengMDPI AGFoods2304-81582024-12-011324414110.3390/foods13244141Progresses and Prospects on Glucosinolate Detection in Cruciferous PlantsXuaner Li0Dongna Wen1Yafei He2Yumei Liu3Fengqing Han4Jialin Su5Shangxiang Lai6Mu Zhuang7Fuxing Gao8Zhansheng Li9State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThis review provides a comprehensive summary of the latest international research on detection methods for glucosinolates in cruciferous plants. This article examines various analytical techniques, including high-performance liquid chromatography (HPLC), liquid chromatography–mass spectrometry (LC-MS), enzyme-linked immunosorbent assay (ELISA), and capillary electrophoresis (CE), while highlighting their respective advantages and limitations. Additionally, this review delves into recent advancements in sample preparation, extraction, and quantification methods, offering valuable insights into the accurate and efficient determination of glucosinolate content across diverse plant materials. Furthermore, it underscores the critical importance of the standardization and validation of these methodologies to ensure reliable glucosinolate analyses in both scientific research and industrial applications.https://www.mdpi.com/2304-8158/13/24/4141glucosinolatecruciferous cropsHPLCLC-MS/MSdetermination
spellingShingle Xuaner Li
Dongna Wen
Yafei He
Yumei Liu
Fengqing Han
Jialin Su
Shangxiang Lai
Mu Zhuang
Fuxing Gao
Zhansheng Li
Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants
Foods
glucosinolate
cruciferous crops
HPLC
LC-MS/MS
determination
title Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants
title_full Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants
title_fullStr Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants
title_full_unstemmed Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants
title_short Progresses and Prospects on Glucosinolate Detection in Cruciferous Plants
title_sort progresses and prospects on glucosinolate detection in cruciferous plants
topic glucosinolate
cruciferous crops
HPLC
LC-MS/MS
determination
url https://www.mdpi.com/2304-8158/13/24/4141
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