SARS-CoV-2 Genomic Surveillance from Community-Distributed Rapid Antigen Tests, Wisconsin, USA
In the United States, SARS-CoV-2 genomic surveillance initially relied almost entirely on residual diagnostic specimens from nucleic acid amplification–based tests. However, use of those tests waned after the end of the COVID-19 Public Health Emergency on May 11, 2023. In Dane County, Wisconsin, we...
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| Language: | English |
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Centers for Disease Control and Prevention
2025-05-01
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| Series: | Emerging Infectious Diseases |
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| Online Access: | https://wwwnc.cdc.gov/eid/article/31/13/24-1192_article |
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| author | Isla E. Emmen William C. Vuyk Andrew J. Lail Sydney Wolf Eli J. O’Connor Rhea Dalvie Maansi Bhasin Aanya Virdi Caroline White Nura R. Hassan Alex Richardson Grace VanSleet Andrea Weiler Savannah Rounds-Dunn Kenneth Van Horn Marc Gartler Jane Jorgenson Michael Spelman Sean Ottosen Nicholas R. Minor Nancy Wilson Thomas C. Friedrich David H. O’Connor |
| author_facet | Isla E. Emmen William C. Vuyk Andrew J. Lail Sydney Wolf Eli J. O’Connor Rhea Dalvie Maansi Bhasin Aanya Virdi Caroline White Nura R. Hassan Alex Richardson Grace VanSleet Andrea Weiler Savannah Rounds-Dunn Kenneth Van Horn Marc Gartler Jane Jorgenson Michael Spelman Sean Ottosen Nicholas R. Minor Nancy Wilson Thomas C. Friedrich David H. O’Connor |
| author_sort | Isla E. Emmen |
| collection | DOAJ |
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In the United States, SARS-CoV-2 genomic surveillance initially relied almost entirely on residual diagnostic specimens from nucleic acid amplification–based tests. However, use of those tests waned after the end of the COVID-19 Public Health Emergency on May 11, 2023. In Dane County, Wisconsin, we partnered with local- and state-level public health agencies and the South Central Library System to continue genomic surveillance by obtaining SARS-CoV-2 genome sequences from freely available community rapid antigen tests (RATs). During August 15, 2023–February 29, 2024, we received 227 RAT samples, from which we generated 127 sequences with >10× depth of coverage for >90% of the SARS-CoV-2 genome. In a subset of tests, lower cycle threshold values correlated with sequence success. Our results demonstrated that collecting and sequencing results from RATs in partnership with community sites is a practical approach for sustaining SARS-CoV-2 genomic surveillance.
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| format | Article |
| id | doaj-art-df9535f8df8643fca0fb622bf3f1c838 |
| institution | OA Journals |
| issn | 1080-6040 1080-6059 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj-art-df9535f8df8643fca0fb622bf3f1c8382025-08-20T01:50:16ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592025-05-013113616910.3201/eid3113.241192SARS-CoV-2 Genomic Surveillance from Community-Distributed Rapid Antigen Tests, Wisconsin, USAIsla E. EmmenWilliam C. VuykAndrew J. LailSydney WolfEli J. O’ConnorRhea DalvieMaansi BhasinAanya VirdiCaroline WhiteNura R. HassanAlex RichardsonGrace VanSleetAndrea WeilerSavannah Rounds-DunnKenneth Van HornMarc GartlerJane JorgensonMichael SpelmanSean OttosenNicholas R. MinorNancy WilsonThomas C. FriedrichDavid H. O’Connor In the United States, SARS-CoV-2 genomic surveillance initially relied almost entirely on residual diagnostic specimens from nucleic acid amplification–based tests. However, use of those tests waned after the end of the COVID-19 Public Health Emergency on May 11, 2023. In Dane County, Wisconsin, we partnered with local- and state-level public health agencies and the South Central Library System to continue genomic surveillance by obtaining SARS-CoV-2 genome sequences from freely available community rapid antigen tests (RATs). During August 15, 2023–February 29, 2024, we received 227 RAT samples, from which we generated 127 sequences with >10× depth of coverage for >90% of the SARS-CoV-2 genome. In a subset of tests, lower cycle threshold values correlated with sequence success. Our results demonstrated that collecting and sequencing results from RATs in partnership with community sites is a practical approach for sustaining SARS-CoV-2 genomic surveillance. https://wwwnc.cdc.gov/eid/article/31/13/24-1192_articleSARS-CoV-2COVID-19respiratory infectionsvirusessevere acute respiratory syndrome coronavirus 2SARS |
| spellingShingle | Isla E. Emmen William C. Vuyk Andrew J. Lail Sydney Wolf Eli J. O’Connor Rhea Dalvie Maansi Bhasin Aanya Virdi Caroline White Nura R. Hassan Alex Richardson Grace VanSleet Andrea Weiler Savannah Rounds-Dunn Kenneth Van Horn Marc Gartler Jane Jorgenson Michael Spelman Sean Ottosen Nicholas R. Minor Nancy Wilson Thomas C. Friedrich David H. O’Connor SARS-CoV-2 Genomic Surveillance from Community-Distributed Rapid Antigen Tests, Wisconsin, USA Emerging Infectious Diseases SARS-CoV-2 COVID-19 respiratory infections viruses severe acute respiratory syndrome coronavirus 2 SARS |
| title | SARS-CoV-2 Genomic Surveillance from Community-Distributed Rapid Antigen Tests, Wisconsin, USA |
| title_full | SARS-CoV-2 Genomic Surveillance from Community-Distributed Rapid Antigen Tests, Wisconsin, USA |
| title_fullStr | SARS-CoV-2 Genomic Surveillance from Community-Distributed Rapid Antigen Tests, Wisconsin, USA |
| title_full_unstemmed | SARS-CoV-2 Genomic Surveillance from Community-Distributed Rapid Antigen Tests, Wisconsin, USA |
| title_short | SARS-CoV-2 Genomic Surveillance from Community-Distributed Rapid Antigen Tests, Wisconsin, USA |
| title_sort | sars cov 2 genomic surveillance from community distributed rapid antigen tests wisconsin usa |
| topic | SARS-CoV-2 COVID-19 respiratory infections viruses severe acute respiratory syndrome coronavirus 2 SARS |
| url | https://wwwnc.cdc.gov/eid/article/31/13/24-1192_article |
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