Pig embryonic stem cells with porcine specific-MSGN1 upstream region-based reporter system for monitoring paraxial mesoderm differentiation
Pig embryonic stem cells were introduced with an MSGN1 upstream region-based reporter system, composed of EGFP sequence under porcine-specific MSGN1 promoter (pMSGN1), through electroporation. The engineered cell line maintained pluripotency similar to that of the parental cell line, and reporter ac...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506125000741 |
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| _version_ | 1849724386981969920 |
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| author | Jinsol Jeong Kwang-Hwan Choi Dong-Kyung Lee Yelim Ahn Chang-Kyu Lee |
| author_facet | Jinsol Jeong Kwang-Hwan Choi Dong-Kyung Lee Yelim Ahn Chang-Kyu Lee |
| author_sort | Jinsol Jeong |
| collection | DOAJ |
| description | Pig embryonic stem cells were introduced with an MSGN1 upstream region-based reporter system, composed of EGFP sequence under porcine-specific MSGN1 promoter (pMSGN1), through electroporation. The engineered cell line maintained pluripotency similar to that of the parental cell line, and reporter activity was confirmed upon the induction of the paraxial mesoderm. The pMSGN1-EGFP reporter system is a valuable tool for monitoring the differentiation process of the paraxial mesoderm and analyzing induction efficiency, providing insight into developmental mechanisms. |
| format | Article |
| id | doaj-art-df91067a85164f76af8dabbeaa25d76a |
| institution | DOAJ |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-df91067a85164f76af8dabbeaa25d76a2025-08-20T03:10:46ZengElsevierStem Cell Research1873-50612025-08-018610372410.1016/j.scr.2025.103724Pig embryonic stem cells with porcine specific-MSGN1 upstream region-based reporter system for monitoring paraxial mesoderm differentiationJinsol Jeong0Kwang-Hwan Choi1Dong-Kyung Lee2Yelim Ahn3Chang-Kyu Lee4Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, South KoreaResearch and Development Center, Space F Corporation, Hwaseong 18471 Gyeonggi-do, South KoreaResearch and Development Center, Space F Corporation, Hwaseong 18471 Gyeonggi-do, South KoreaDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, South KoreaDepartment of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, South Korea; Institute of Green Bio Science and Technology, Seoul National University, Pyeong Chang, Kangwon-do 25354, South Korea; Corresponding author at: Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 08826, South Korea.Pig embryonic stem cells were introduced with an MSGN1 upstream region-based reporter system, composed of EGFP sequence under porcine-specific MSGN1 promoter (pMSGN1), through electroporation. The engineered cell line maintained pluripotency similar to that of the parental cell line, and reporter activity was confirmed upon the induction of the paraxial mesoderm. The pMSGN1-EGFP reporter system is a valuable tool for monitoring the differentiation process of the paraxial mesoderm and analyzing induction efficiency, providing insight into developmental mechanisms.http://www.sciencedirect.com/science/article/pii/S1873506125000741 |
| spellingShingle | Jinsol Jeong Kwang-Hwan Choi Dong-Kyung Lee Yelim Ahn Chang-Kyu Lee Pig embryonic stem cells with porcine specific-MSGN1 upstream region-based reporter system for monitoring paraxial mesoderm differentiation Stem Cell Research |
| title | Pig embryonic stem cells with porcine specific-MSGN1 upstream region-based reporter system for monitoring paraxial mesoderm differentiation |
| title_full | Pig embryonic stem cells with porcine specific-MSGN1 upstream region-based reporter system for monitoring paraxial mesoderm differentiation |
| title_fullStr | Pig embryonic stem cells with porcine specific-MSGN1 upstream region-based reporter system for monitoring paraxial mesoderm differentiation |
| title_full_unstemmed | Pig embryonic stem cells with porcine specific-MSGN1 upstream region-based reporter system for monitoring paraxial mesoderm differentiation |
| title_short | Pig embryonic stem cells with porcine specific-MSGN1 upstream region-based reporter system for monitoring paraxial mesoderm differentiation |
| title_sort | pig embryonic stem cells with porcine specific msgn1 upstream region based reporter system for monitoring paraxial mesoderm differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S1873506125000741 |
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