Anti‐inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF‐κB and MAPK signaling pathways
Medicinal plants contain bioactive compounds that have therapeutic effects on human health. Ophioglossum vulgatum L. is a representative species of the fern genus Ophioglossum that has anti‐inflammatory properties as recognized in folk medicine. Herein, we performed a nitric oxide (NO) assay‐guided...
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2025-01-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13914 |
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| author | Xiaoqing Zhu Cheng Tian Dan Yao Siqi Li Junjiang Lv Yongwen Chen Xiaoyong Huang |
| author_facet | Xiaoqing Zhu Cheng Tian Dan Yao Siqi Li Junjiang Lv Yongwen Chen Xiaoyong Huang |
| author_sort | Xiaoqing Zhu |
| collection | DOAJ |
| description | Medicinal plants contain bioactive compounds that have therapeutic effects on human health. Ophioglossum vulgatum L. is a representative species of the fern genus Ophioglossum that has anti‐inflammatory properties as recognized in folk medicine. Herein, we performed a nitric oxide (NO) assay‐guided screening in RAW264.7 cells to investigate the active components of the plant. We found that ophioglonin (OPN), a characteristic homoflavonoid of the genus Ophioglossum, is one of the bioactive components. Therefore, we performed a comparative analysis of the isolated compounds and found that OPN has effects similar to those of isolated dihydroquercetin and luteolin at the concentrations tested. The antioxidant and anti‐inflammatory activities of OPN were extensively validated using lipopolysaccharide ‐stimulated RAW264.7 cells, mouse bone marrow‐derived macrophages (BMDMs), and peritoneal exudate macrophages (PEMs). In vivo experiments with a carrageenan‐induced mouse paw edema model further confirmed the anti‐inflammatory effect of OPN. Additionally, we found that OPN and Ophioglossum vulgatum extracts inhibit the activation of signal transducers, NF‐ĸB p65, IĸBα, ERK, p38, and JNK, consistent with the findings of pathway enrichment analysis. This work reinforces the anti‐inflammatory properties of Ophioglossum vulgatum and indicates that OPN is a promising therapeutic agent for inflammation‐associated disorders. Further clinical evaluations, including clinical trials, would be beneficial to validate the anti‐inflammatory properties of OPN. |
| format | Article |
| id | doaj-art-df84de662edd41a182653046e688e74c |
| institution | Kabale University |
| issn | 2211-5463 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-df84de662edd41a182653046e688e74c2025-01-07T02:27:34ZengWileyFEBS Open Bio2211-54632025-01-0115112213910.1002/2211-5463.13914Anti‐inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF‐κB and MAPK signaling pathwaysXiaoqing Zhu0Cheng Tian1Dan Yao2Siqi Li3Junjiang Lv4Yongwen Chen5Xiaoyong Huang6Science and Technology Industry Development Center, Chongqing Medical and Pharmaceutical College Chongqing ChinaScience and Technology Industry Development Center, Chongqing Medical and Pharmaceutical College Chongqing ChinaScience and Technology Industry Development Center, Chongqing Medical and Pharmaceutical College Chongqing ChinaInstitute of Immunology, People's Liberation Army Third Military Medical University Chongqing ChinaScience and Technology Industry Development Center, Chongqing Medical and Pharmaceutical College Chongqing ChinaInstitute of Immunology, People's Liberation Army Third Military Medical University Chongqing ChinaInstitute of Immunology, People's Liberation Army Third Military Medical University Chongqing ChinaMedicinal plants contain bioactive compounds that have therapeutic effects on human health. Ophioglossum vulgatum L. is a representative species of the fern genus Ophioglossum that has anti‐inflammatory properties as recognized in folk medicine. Herein, we performed a nitric oxide (NO) assay‐guided screening in RAW264.7 cells to investigate the active components of the plant. We found that ophioglonin (OPN), a characteristic homoflavonoid of the genus Ophioglossum, is one of the bioactive components. Therefore, we performed a comparative analysis of the isolated compounds and found that OPN has effects similar to those of isolated dihydroquercetin and luteolin at the concentrations tested. The antioxidant and anti‐inflammatory activities of OPN were extensively validated using lipopolysaccharide ‐stimulated RAW264.7 cells, mouse bone marrow‐derived macrophages (BMDMs), and peritoneal exudate macrophages (PEMs). In vivo experiments with a carrageenan‐induced mouse paw edema model further confirmed the anti‐inflammatory effect of OPN. Additionally, we found that OPN and Ophioglossum vulgatum extracts inhibit the activation of signal transducers, NF‐ĸB p65, IĸBα, ERK, p38, and JNK, consistent with the findings of pathway enrichment analysis. This work reinforces the anti‐inflammatory properties of Ophioglossum vulgatum and indicates that OPN is a promising therapeutic agent for inflammation‐associated disorders. Further clinical evaluations, including clinical trials, would be beneficial to validate the anti‐inflammatory properties of OPN.https://doi.org/10.1002/2211-5463.13914anti‐inflammationantioxidantmacrophagesmechanismOphiogloninOphioglossum vulgatum |
| spellingShingle | Xiaoqing Zhu Cheng Tian Dan Yao Siqi Li Junjiang Lv Yongwen Chen Xiaoyong Huang Anti‐inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF‐κB and MAPK signaling pathways FEBS Open Bio anti‐inflammation antioxidant macrophages mechanism Ophioglonin Ophioglossum vulgatum |
| title | Anti‐inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF‐κB and MAPK signaling pathways |
| title_full | Anti‐inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF‐κB and MAPK signaling pathways |
| title_fullStr | Anti‐inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF‐κB and MAPK signaling pathways |
| title_full_unstemmed | Anti‐inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF‐κB and MAPK signaling pathways |
| title_short | Anti‐inflammatory properties of ophioglonin derived from the fern Ophioglossum vulgatum L. via inactivating NF‐κB and MAPK signaling pathways |
| title_sort | anti inflammatory properties of ophioglonin derived from the fern ophioglossum vulgatum l via inactivating nf κb and mapk signaling pathways |
| topic | anti‐inflammation antioxidant macrophages mechanism Ophioglonin Ophioglossum vulgatum |
| url | https://doi.org/10.1002/2211-5463.13914 |
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