A PCR primer design method for identifying spider mite species using k-mer counting.

A PCR primer design method for identifying spider mite species using k-mer counting.

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Using PCR to distinguish closely related species can be difficult because they may have very similar genomes. Advances in bioinformatics make it possible to design PCR primers that are species-specific. In this study, we developed a bioinformatics method for extracting species-specific primer candid...

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Bibliographic Details
Main Authors: Tomoko Matsuda, Hironori Sakamoto, Takumi Kayukawa, Yasuki Kitashima, Toshinori Kozaki, Tetsuo Gotoh
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0321199
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