Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs
IntroductionHuman endometrial mesenchymal stem cells (H-EMSCs) can inhibit endometrial fibrosis and repair damaged endometrium. However, direct cell injection into dam-aged endometrium shows limited cell survival. Cell seeding onto biomaterial-based electrospun membranes could improve H-EMSCs’ survi...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1551791/full |
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| author | Jiangru An Tianyi Ma Qiuhua Wang Jinyi Zhang J. Paul Santerre J. Paul Santerre Wenshuang Wang Peng Ma Xiaoqing Zhang Xiaoqing Zhang |
| author_facet | Jiangru An Tianyi Ma Qiuhua Wang Jinyi Zhang J. Paul Santerre J. Paul Santerre Wenshuang Wang Peng Ma Xiaoqing Zhang Xiaoqing Zhang |
| author_sort | Jiangru An |
| collection | DOAJ |
| description | IntroductionHuman endometrial mesenchymal stem cells (H-EMSCs) can inhibit endometrial fibrosis and repair damaged endometrium. However, direct cell injection into dam-aged endometrium shows limited cell survival. Cell seeding onto biomaterial-based electrospun membranes could improve H-EMSCs’ survival and prolong their stay at the damaged endometrium. Polycaprolactone (PCL), silk fibroin (SF) and hyaluronic acid (HA) are synthetic or natural biomaterials used by the biomedicine field, however, their effects on the biological activities of H-EMSCs remain unclear.MethodsIn this study, CD90+CD73+CD45− H-EMSCs were extracted from human endometrium and H-EMSCs showed enhanced adhesion, proliferation on PCL-HA vs. PCL, PCL-SF, establishing the potential of the composite to address cell survival issues.ResultsH-EMSCs cultured on PCL-HA showed decreased IL-6 gene expression and increased IL-10, VEGFA, TGF-β gene expression vs. PCL-SF, establishing the potential to create a favorable micro-environment for generating vascularized endometrial tissues. PCL, PCL-SF, PCL-HA all supported CD90 and Meflin expression of the seeded H-EMSCs, establishing PCL as a platform to form enhanced biomaterial composites for endometrial repair in the future.DiscussionThis study provided significant evidence sup-porting the potential of appropriately tailored composites of PCL and HA to moder-ate inflammation and wound-healing, which can be applied for endometrial tissue repair and regeneration. |
| format | Article |
| id | doaj-art-df7caadd532e448bb8d0e30a4fa9ee01 |
| institution | DOAJ |
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| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-df7caadd532e448bb8d0e30a4fa9ee012025-08-20T03:12:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-02-011310.3389/fbioe.2025.15517911551791Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCsJiangru An0Tianyi Ma1Qiuhua Wang2Jinyi Zhang3J. Paul Santerre4J. Paul Santerre5Wenshuang Wang6Peng Ma7Xiaoqing Zhang8Xiaoqing Zhang9International Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong, ChinaLi Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, ChinaInternational Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong, ChinaInternational Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong, ChinaInternational Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong, ChinaInstitute of Biomedical Engineering, University of Toronto, Toronto, ON, CanadaDepartment of Gynecology, Yuhuangding Hospital, Yantai, Shandong, ChinaInternational Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong, ChinaInternational Joint Laboratory of Biomaterials and Tissue Regeneration, School of Basic Medicine, Binzhou Medical University, Yantai, Shandong, ChinaInstitute of Biomedical Engineering, University of Toronto, Toronto, ON, CanadaIntroductionHuman endometrial mesenchymal stem cells (H-EMSCs) can inhibit endometrial fibrosis and repair damaged endometrium. However, direct cell injection into dam-aged endometrium shows limited cell survival. Cell seeding onto biomaterial-based electrospun membranes could improve H-EMSCs’ survival and prolong their stay at the damaged endometrium. Polycaprolactone (PCL), silk fibroin (SF) and hyaluronic acid (HA) are synthetic or natural biomaterials used by the biomedicine field, however, their effects on the biological activities of H-EMSCs remain unclear.MethodsIn this study, CD90+CD73+CD45− H-EMSCs were extracted from human endometrium and H-EMSCs showed enhanced adhesion, proliferation on PCL-HA vs. PCL, PCL-SF, establishing the potential of the composite to address cell survival issues.ResultsH-EMSCs cultured on PCL-HA showed decreased IL-6 gene expression and increased IL-10, VEGFA, TGF-β gene expression vs. PCL-SF, establishing the potential to create a favorable micro-environment for generating vascularized endometrial tissues. PCL, PCL-SF, PCL-HA all supported CD90 and Meflin expression of the seeded H-EMSCs, establishing PCL as a platform to form enhanced biomaterial composites for endometrial repair in the future.DiscussionThis study provided significant evidence sup-porting the potential of appropriately tailored composites of PCL and HA to moder-ate inflammation and wound-healing, which can be applied for endometrial tissue repair and regeneration.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1551791/fullhuman endometrial mesenchymal stem cellsbiomaterial electrospun membranesPoly-caprolactonesilk fibroinhyaluronic acid |
| spellingShingle | Jiangru An Tianyi Ma Qiuhua Wang Jinyi Zhang J. Paul Santerre J. Paul Santerre Wenshuang Wang Peng Ma Xiaoqing Zhang Xiaoqing Zhang Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs Frontiers in Bioengineering and Biotechnology human endometrial mesenchymal stem cells biomaterial electrospun membranes Poly-caprolactone silk fibroin hyaluronic acid |
| title | Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs |
| title_full | Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs |
| title_fullStr | Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs |
| title_full_unstemmed | Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs |
| title_short | Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs |
| title_sort | defining optimal electrospun membranes to enhance biological activities of human endometrial mscs |
| topic | human endometrial mesenchymal stem cells biomaterial electrospun membranes Poly-caprolactone silk fibroin hyaluronic acid |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1551791/full |
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