The <i>stn1</i>-<i>sz2</i> Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression
The conserved and essential Cdc13/CTC1-Stn1-Ten1 telomeric complex (CST) ensures chromosome stability by protecting telomere ends and regulating telomerase accessibility. In a recent study, we uncovered mutants of the <i>S</i>. <i>cerevisiae</i> CST, in which damage was sense...
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2025-05-01
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| author | Nathalie Grandin Michel Charbonneau |
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| description | The conserved and essential Cdc13/CTC1-Stn1-Ten1 telomeric complex (CST) ensures chromosome stability by protecting telomere ends and regulating telomerase accessibility. In a recent study, we uncovered mutants of the <i>S</i>. <i>cerevisiae</i> CST, in which damage was sensed by the two major G2/M spindle checkpoints (one is Bub2-dependent and the other one Mad2-dependent), as well as the major G2/M DNA damage checkpoint (Mec1-dependent). In this study, we found, by fluorescence microscopy, that the stability of the mitotic tubulin spindle was profoundly affected in the best-studied of these mutants, <i>stn1</i>-<i>sz2</i>. Additional data from genetic analyses suggested the potential involvement of Stu1 and Stu2, as well as Slk19, in these defects. Throughout this study, we compared the phenotypes of <i>stn1</i>-<i>sz2</i> with those of <i>cdc13</i>-<i>1</i>, the best-studied CST mutant, which also serves as a prototype of telomere-damage-characterized CST mutants. We propose that <i>stn1</i>-<i>sz2</i> represents the prototype of <i>cst</i> mutants characterized by tubulin spindle damage. These newly described phenotypes potentially represent the basis for identifying new functions of the CST telomeric complex. These functions might consist of ensuring correct chromosome segregation through the stabilization of the mitotic spindle. |
| format | Article |
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| spelling | doaj-art-df6f85d1df00485fa7e23c2207d3943f2025-08-20T02:33:08ZengMDPI AGCells2073-44092025-05-01141178410.3390/cells14110784The <i>stn1</i>-<i>sz2</i> Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle ProgressionNathalie Grandin0Michel Charbonneau1GReD Institute, CNRS UMR6293, INSERM U1103, Faculty of Medicine, University Clermont-Auvergne, 28 Place Henri Dunant, BP 38, 63001 Clermont-Ferrand Cedex, FranceGReD Institute, CNRS UMR6293, INSERM U1103, Faculty of Medicine, University Clermont-Auvergne, 28 Place Henri Dunant, BP 38, 63001 Clermont-Ferrand Cedex, FranceThe conserved and essential Cdc13/CTC1-Stn1-Ten1 telomeric complex (CST) ensures chromosome stability by protecting telomere ends and regulating telomerase accessibility. In a recent study, we uncovered mutants of the <i>S</i>. <i>cerevisiae</i> CST, in which damage was sensed by the two major G2/M spindle checkpoints (one is Bub2-dependent and the other one Mad2-dependent), as well as the major G2/M DNA damage checkpoint (Mec1-dependent). In this study, we found, by fluorescence microscopy, that the stability of the mitotic tubulin spindle was profoundly affected in the best-studied of these mutants, <i>stn1</i>-<i>sz2</i>. Additional data from genetic analyses suggested the potential involvement of Stu1 and Stu2, as well as Slk19, in these defects. Throughout this study, we compared the phenotypes of <i>stn1</i>-<i>sz2</i> with those of <i>cdc13</i>-<i>1</i>, the best-studied CST mutant, which also serves as a prototype of telomere-damage-characterized CST mutants. We propose that <i>stn1</i>-<i>sz2</i> represents the prototype of <i>cst</i> mutants characterized by tubulin spindle damage. These newly described phenotypes potentially represent the basis for identifying new functions of the CST telomeric complex. These functions might consist of ensuring correct chromosome segregation through the stabilization of the mitotic spindle.https://www.mdpi.com/2073-4409/14/11/784budding yeast telomeresCdc13-Stn1-Ten1 complexcell cycleBub2 and Mad2 spindle checkpointsMec1 DNA damage checkpoint |
| spellingShingle | Nathalie Grandin Michel Charbonneau The <i>stn1</i>-<i>sz2</i> Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression Cells budding yeast telomeres Cdc13-Stn1-Ten1 complex cell cycle Bub2 and Mad2 spindle checkpoints Mec1 DNA damage checkpoint |
| title | The <i>stn1</i>-<i>sz2</i> Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression |
| title_full | The <i>stn1</i>-<i>sz2</i> Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression |
| title_fullStr | The <i>stn1</i>-<i>sz2</i> Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression |
| title_full_unstemmed | The <i>stn1</i>-<i>sz2</i> Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression |
| title_short | The <i>stn1</i>-<i>sz2</i> Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression |
| title_sort | i stn1 i i sz2 i mutant provides new insight into the impacts of telomeric cdc13 stn1 ten1 dysfunction on cell cycle progression |
| topic | budding yeast telomeres Cdc13-Stn1-Ten1 complex cell cycle Bub2 and Mad2 spindle checkpoints Mec1 DNA damage checkpoint |
| url | https://www.mdpi.com/2073-4409/14/11/784 |
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