Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavities
Abstract Background A didelphic uterus represents a unique and infrequent congenital condition in which a woman possesses two distinct uteri, each with its own cervix. This anomaly arises due to partial or incomplete merging of the Müllerian ducts during the developmental stages in the womb. Account...
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BMC
2025-01-01
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| Series: | Reproductive Biology and Endocrinology |
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| Online Access: | https://doi.org/10.1186/s12958-024-01330-7 |
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| author | Alberto Sola-Leyva Bárbara Romero Analuce Canha-Gouveia Inmaculada Pérez-Prieto Nerea M. Molina Eva Vargas Juan Mozas-Moreno Clara Chamorro Merli Saare Andres Salumets Signe Altmäe |
| author_facet | Alberto Sola-Leyva Bárbara Romero Analuce Canha-Gouveia Inmaculada Pérez-Prieto Nerea M. Molina Eva Vargas Juan Mozas-Moreno Clara Chamorro Merli Saare Andres Salumets Signe Altmäe |
| author_sort | Alberto Sola-Leyva |
| collection | DOAJ |
| description | Abstract Background A didelphic uterus represents a unique and infrequent congenital condition in which a woman possesses two distinct uteri, each with its own cervix. This anomaly arises due to partial or incomplete merging of the Müllerian ducts during the developmental stages in the womb. Accounting for uterine malformations, a didelphic uterus is a relatively rare condition, affecting approximately 0.5–2% of the population and is considered one of the more uncommon types of uterine abnormalities. Methods This case report aims to study the physical separation in uterine didelphys and its impact on endometrial microbiome and inflammation, and the patterns of endometrial receptivity observed. Results Endometrial receptivity analyses revealed a similar receptive state in both uteri, both in the early receptive phase. Differential markers of chronic endometritis, including CD138, and MUM1-positive cells, were observed when comparing endometrial biopsies from both uteri. The right uterus exhibited a higher prevalence of these positive cells. Regarding the microbiome, significant differences were found between the uteri, notably in the right uterus, a clear non-dominance of lactobacilli and the presence of genera such as Staphylococcus, Streptococcus, and Acinetobacter. Additionally, the right uterus presented a less ‘favourable’ microenvironment, a characteristic that was also reflected in the right cervix; both sites presenting less lactobacilli than the left side samples. A distinct metabolomic signature associated with the physical separation of the uteri contributed to the differences in endometrial milieu. Conclusions Our study revealed that physical separation, among other factors in uterus didelphys, affects the endometrial microbiome, metabolome, and inflammatory state, with significant microbiome variation observed between the uteri, although similar endometrial receptivity patterns were noted. |
| format | Article |
| id | doaj-art-df6eb6c4261d4802878d7439d03eb9b7 |
| institution | Kabale University |
| issn | 1477-7827 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj-art-df6eb6c4261d4802878d7439d03eb9b72025-01-05T12:50:12ZengBMCReproductive Biology and Endocrinology1477-78272025-01-0123111010.1186/s12958-024-01330-7Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavitiesAlberto Sola-Leyva0Bárbara Romero1Analuce Canha-Gouveia2Inmaculada Pérez-Prieto3Nerea M. Molina4Eva Vargas5Juan Mozas-Moreno6Clara Chamorro7Merli Saare8Andres Salumets9Signe Altmäe10Department of Gynecology and Reproductive Medicine, Karolinska University HospitalReproduction Unit, UGC Obstetrics and Gynaecology, University Hospital Virgen de las NievesInstituto de Investigación Biosanitaria ibs.GRANADAInstituto de Investigación Biosanitaria ibs.GRANADAInstituto de Investigación Biosanitaria ibs.GRANADAInstituto de Investigación Biosanitaria ibs.GRANADAInstituto de Investigación Biosanitaria ibs.GRANADAUnidad Provincial de Anatomía Patológica, Hospital Virgen de las NievesCelvia CC, Competence Centre on Health TechnologiesDepartment of Gynecology and Reproductive Medicine, Karolinska University HospitalDepartment of Gynecology and Reproductive Medicine, Karolinska University HospitalAbstract Background A didelphic uterus represents a unique and infrequent congenital condition in which a woman possesses two distinct uteri, each with its own cervix. This anomaly arises due to partial or incomplete merging of the Müllerian ducts during the developmental stages in the womb. Accounting for uterine malformations, a didelphic uterus is a relatively rare condition, affecting approximately 0.5–2% of the population and is considered one of the more uncommon types of uterine abnormalities. Methods This case report aims to study the physical separation in uterine didelphys and its impact on endometrial microbiome and inflammation, and the patterns of endometrial receptivity observed. Results Endometrial receptivity analyses revealed a similar receptive state in both uteri, both in the early receptive phase. Differential markers of chronic endometritis, including CD138, and MUM1-positive cells, were observed when comparing endometrial biopsies from both uteri. The right uterus exhibited a higher prevalence of these positive cells. Regarding the microbiome, significant differences were found between the uteri, notably in the right uterus, a clear non-dominance of lactobacilli and the presence of genera such as Staphylococcus, Streptococcus, and Acinetobacter. Additionally, the right uterus presented a less ‘favourable’ microenvironment, a characteristic that was also reflected in the right cervix; both sites presenting less lactobacilli than the left side samples. A distinct metabolomic signature associated with the physical separation of the uteri contributed to the differences in endometrial milieu. Conclusions Our study revealed that physical separation, among other factors in uterus didelphys, affects the endometrial microbiome, metabolome, and inflammatory state, with significant microbiome variation observed between the uteri, although similar endometrial receptivity patterns were noted.https://doi.org/10.1186/s12958-024-01330-7Uterus didelphysMicrobiomeMetabolomeChronic endometritisEndometrial receptivity |
| spellingShingle | Alberto Sola-Leyva Bárbara Romero Analuce Canha-Gouveia Inmaculada Pérez-Prieto Nerea M. Molina Eva Vargas Juan Mozas-Moreno Clara Chamorro Merli Saare Andres Salumets Signe Altmäe Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavities Reproductive Biology and Endocrinology Uterus didelphys Microbiome Metabolome Chronic endometritis Endometrial receptivity |
| title | Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavities |
| title_full | Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavities |
| title_fullStr | Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavities |
| title_full_unstemmed | Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavities |
| title_short | Uterus didelphys: the first case report on molecular profiling of endometrial tissue from both uterine cavities |
| title_sort | uterus didelphys the first case report on molecular profiling of endometrial tissue from both uterine cavities |
| topic | Uterus didelphys Microbiome Metabolome Chronic endometritis Endometrial receptivity |
| url | https://doi.org/10.1186/s12958-024-01330-7 |
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