Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent Assay
Aim: Evaluation of interrelation between vitamin D levels in saliva and early childhood caries (ECC) utilizing enzyme-linked immunosorbent assay (ELISA). Materials and methods: The sample selection in the cross-sectional, case-control study involved—(1) an interview with the parents/caregivers throu...
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Jaypee Brothers Medical Publisher
2023-12-01
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| Series: | Journal of South Asian Association of Pediatric Dentistry |
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| Online Access: | https://www.jsaapd.com/doi/JSAAPD/pdf/10.5005/jp-journals-10077-3284 |
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| author | Umme Azher Aishwarya Chidambareshwar Santhosh T Paul Divya Reddy |
| author_facet | Umme Azher Aishwarya Chidambareshwar Santhosh T Paul Divya Reddy |
| author_sort | Umme Azher |
| collection | DOAJ |
| description | Aim: Evaluation of interrelation between vitamin D levels in saliva and early childhood caries (ECC) utilizing enzyme-linked immunosorbent assay (ELISA).
Materials and methods: The sample selection in the cross-sectional, case-control study involved—(1) an interview with the parents/caregivers through a questionnaire; (2) a clinical examination of children to ascertain dental decay status; those with severe ECC (S-ECC) were enlisted in group I (<italic>n</italic> = 15) and children with a decayed, missing, and filled surfaces (dmfs) score of 0 to group II (<italic>n</italic> = 15). The samples of unstimulated whole saliva (5 mL) were collected, and the salivary vitamin D levels were measured using a human 25-dihydroxy vitamin D<sub>3</sub> (25-OHD) ELISA kit.
Results: Subjects in groups I and II showed mean salivary vitamin D levels of 8.8 + 4.92 and 16.8 + 10.8 ng/mL, respectively. The vitamin D levels in saliva exhibited a difference statistically.
Conclusion: Variations in vitamin D scores in the saliva of children with S-ECC and those caries-free demonstrate that vitamin D in saliva can serve as a marker of caries risk in children.
Clinical significance: The simplicity and noninvasiveness of saliva collection, especially in children, confers the advantage of a more acceptable and cost-effective biofluid for caries-risk factors assessment. |
| format | Article |
| id | doaj-art-df6b8e162c9847139bdc647d43e696af |
| institution | OA Journals |
| issn | 2582-1024 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Jaypee Brothers Medical Publisher |
| record_format | Article |
| series | Journal of South Asian Association of Pediatric Dentistry |
| spelling | doaj-art-df6b8e162c9847139bdc647d43e696af2025-08-20T02:27:50ZengJaypee Brothers Medical PublisherJournal of South Asian Association of Pediatric Dentistry2582-10242023-12-016311411710.5005/jp-journals-10077-32844Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent AssayUmme Azher0Aishwarya Chidambareshwar1Santhosh T Paul2Divya Reddy3Umme Azher, Department of Dental, Faculty of Paedodontics and Preventive Dentistry, Sri Rajiv Gandhi College of Dental Sciences & Hospital, Bengaluru, Karnataka, India, Phone: +91 9945352984Department of Pediatric & Preventive Dentistry, Hyderabad, Telangana, IndiaDepartment of Pediatric and Preventive Dentistry, Sri Rajiv Gandhi College of Dental Sciences & Hospital, Bengaluru, Karnataka, IndiaDepartment of Pediatric and Preventive Dentistry, Sri Rajiv Gandhi College of Dental Sciences & Hospital, Bengaluru, Karnataka, IndiaAim: Evaluation of interrelation between vitamin D levels in saliva and early childhood caries (ECC) utilizing enzyme-linked immunosorbent assay (ELISA). Materials and methods: The sample selection in the cross-sectional, case-control study involved—(1) an interview with the parents/caregivers through a questionnaire; (2) a clinical examination of children to ascertain dental decay status; those with severe ECC (S-ECC) were enlisted in group I (<italic>n</italic> = 15) and children with a decayed, missing, and filled surfaces (dmfs) score of 0 to group II (<italic>n</italic> = 15). The samples of unstimulated whole saliva (5 mL) were collected, and the salivary vitamin D levels were measured using a human 25-dihydroxy vitamin D<sub>3</sub> (25-OHD) ELISA kit. Results: Subjects in groups I and II showed mean salivary vitamin D levels of 8.8 + 4.92 and 16.8 + 10.8 ng/mL, respectively. The vitamin D levels in saliva exhibited a difference statistically. Conclusion: Variations in vitamin D scores in the saliva of children with S-ECC and those caries-free demonstrate that vitamin D in saliva can serve as a marker of caries risk in children. Clinical significance: The simplicity and noninvasiveness of saliva collection, especially in children, confers the advantage of a more acceptable and cost-effective biofluid for caries-risk factors assessment.https://www.jsaapd.com/doi/JSAAPD/pdf/10.5005/jp-journals-10077-3284biofluiddental decayearly childhood cariessalivavitamin d |
| spellingShingle | Umme Azher Aishwarya Chidambareshwar Santhosh T Paul Divya Reddy Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent Assay Journal of South Asian Association of Pediatric Dentistry biofluid dental decay early childhood caries saliva vitamin d |
| title | Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent Assay |
| title_full | Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent Assay |
| title_fullStr | Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent Assay |
| title_full_unstemmed | Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent Assay |
| title_short | Comparative Assessment of the Association between Salivary Vitamin D Levels and Early Childhood Caries Using Enzyme-linked Immunosorbent Assay |
| title_sort | comparative assessment of the association between salivary vitamin d levels and early childhood caries using enzyme linked immunosorbent assay |
| topic | biofluid dental decay early childhood caries saliva vitamin d |
| url | https://www.jsaapd.com/doi/JSAAPD/pdf/10.5005/jp-journals-10077-3284 |
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