MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer
We intended to investigate the functional role and clinical relevance of microRNA-125b in human gallbladder cancer. Quantitative real-time polymerase chain reaction was used to examine microRNA-125b expression in gallbladder cancer cell lines, and 79 pairs of gallbladder cancer and normal gallbladde...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2017-03-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317692249 |
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| author | Liang Yang Sheng Huang Hongbin Ma Xiaoxiong Wu Feiling Feng |
| author_facet | Liang Yang Sheng Huang Hongbin Ma Xiaoxiong Wu Feiling Feng |
| author_sort | Liang Yang |
| collection | DOAJ |
| description | We intended to investigate the functional role and clinical relevance of microRNA-125b in human gallbladder cancer. Quantitative real-time polymerase chain reaction was used to examine microRNA-125b expression in gallbladder cancer cell lines, and 79 pairs of gallbladder cancer and normal gallbladder clinical tissues. Clinical correlations between tumorous microRNA-125b expression and gallbladder cancer patients’ clinicopathological variances or overall survivals were statistically analyzed. In gallbladder cancer cell lines, TYGBK-8 and G-415 cells, microRNA-125b was upregulated to examine its regulatory effect on gallbladder cancer proliferation and migration in vitro. MicroRNA-125b was significantly downregulated in gallbladder cancer cell lines and human gallbladder cancer tumors. MicroRNA-125b in gallbladder cancer was significantly correlated with patients’ clinical stage, tumor differentiation, lymph metastasis, and tumor invasion. Low tumorous microRNA-125b expression was also found to be associated with poor overall survivals among gallbladder cancer patients. In vitro studies demonstrated that microRNA-125b upregulation significantly suppressed proliferation and migration in TYGBK-8 and G-415 cells. Tumorous microRNA-125b is an independent prognostic biomarker for patients with gallbladder cancer and possibly acts as a tumor suppressor in gallbladder cancer. |
| format | Article |
| id | doaj-art-df67fb5caabf46ddab76d716dcc8f1fa |
| institution | DOAJ |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-df67fb5caabf46ddab76d716dcc8f1fa2025-08-20T03:18:15ZengSAGE PublishingTumor Biology1423-03802017-03-013910.1177/1010428317692249MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancerLiang Yang0Sheng Huang1Hongbin Ma2Xiaoxiong Wu3Feiling Feng4Co-first authorsCo-first authorsCo-first authorsDepartment of Integrated Traditional Chinese and Western Medicine, East Hepatobiliary Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Biliary Tract, East Hepatobiliary Hospital, Second Military Medical University, Shanghai, ChinaWe intended to investigate the functional role and clinical relevance of microRNA-125b in human gallbladder cancer. Quantitative real-time polymerase chain reaction was used to examine microRNA-125b expression in gallbladder cancer cell lines, and 79 pairs of gallbladder cancer and normal gallbladder clinical tissues. Clinical correlations between tumorous microRNA-125b expression and gallbladder cancer patients’ clinicopathological variances or overall survivals were statistically analyzed. In gallbladder cancer cell lines, TYGBK-8 and G-415 cells, microRNA-125b was upregulated to examine its regulatory effect on gallbladder cancer proliferation and migration in vitro. MicroRNA-125b was significantly downregulated in gallbladder cancer cell lines and human gallbladder cancer tumors. MicroRNA-125b in gallbladder cancer was significantly correlated with patients’ clinical stage, tumor differentiation, lymph metastasis, and tumor invasion. Low tumorous microRNA-125b expression was also found to be associated with poor overall survivals among gallbladder cancer patients. In vitro studies demonstrated that microRNA-125b upregulation significantly suppressed proliferation and migration in TYGBK-8 and G-415 cells. Tumorous microRNA-125b is an independent prognostic biomarker for patients with gallbladder cancer and possibly acts as a tumor suppressor in gallbladder cancer.https://doi.org/10.1177/1010428317692249 |
| spellingShingle | Liang Yang Sheng Huang Hongbin Ma Xiaoxiong Wu Feiling Feng MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer Tumor Biology |
| title | MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer |
| title_full | MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer |
| title_fullStr | MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer |
| title_full_unstemmed | MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer |
| title_short | MicroRNA-125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer |
| title_sort | microrna 125b predicts clinical outcome and suppressed tumor proliferation and migration in human gallbladder cancer |
| url | https://doi.org/10.1177/1010428317692249 |
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