Establishment of a genotyping criteria for Bandavirus dabieense and confirmation of new genotypes
Abstract Bandavirus dabieense (DBV) causes severe fever with thrombocytopenia syndrome, which has a mortality rate of 6.18% of 27%. DBV has been classified into five to six genotypes using phylogenetic analyses. However, the absence of clear standards poses challenges in identifying new genotypes. W...
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Nature Portfolio
2025-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-94203-x |
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| author | Bingyan Liu Tengfei He Changtai Wang Guomei Xia Shijun Zhou Shanshan Sun Xinlei Chen Xiaodan Hong Jie Zhu Zhenhua Zhang |
| author_facet | Bingyan Liu Tengfei He Changtai Wang Guomei Xia Shijun Zhou Shanshan Sun Xinlei Chen Xiaodan Hong Jie Zhu Zhenhua Zhang |
| author_sort | Bingyan Liu |
| collection | DOAJ |
| description | Abstract Bandavirus dabieense (DBV) causes severe fever with thrombocytopenia syndrome, which has a mortality rate of 6.18% of 27%. DBV has been classified into five to six genotypes using phylogenetic analyses. However, the absence of clear standards poses challenges in identifying new genotypes. We performed evolutionary and homology analyses using the sequences from GenBank and analysed nucleotide differences between the different genotypes. Nucleotide differences within the same genotype were mostly below 3%, whereas those between different genotypes ranged from 3 to 7%. Consequently, we established and validated a specific genotyping criterion for DBV using phylogenetic tree analysis with a 3% cut-off value and identified 8, 11, and 11 genotypes in the S, M, and L segments, respectively. Furthermore, we compared our method with the previous genotyping methods to elucidate the convenience and advantages of using a 3% cut-off value. Importantly, we also identified new genotypes and fragment reassortments in DBV using our new genotyping criterion. Additionally, we established two simplified genotyping methods for the rapid typing of DBV in clinical settings and demonstrated the existence of geographical and clinical variations among the different genotypes. Our findings provide a more reliable foundation for clinical typing of DBV. |
| format | Article |
| id | doaj-art-df58dbc030e745efbfbe65fea0fbbab0 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-df58dbc030e745efbfbe65fea0fbbab02025-08-20T03:07:41ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-025-94203-xEstablishment of a genotyping criteria for Bandavirus dabieense and confirmation of new genotypesBingyan Liu0Tengfei He1Changtai Wang2Guomei Xia3Shijun Zhou4Shanshan Sun5Xinlei Chen6Xiaodan Hong7Jie Zhu8Zhenhua Zhang9Department of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical UniversityInstitute of Clinical Virology, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical UniversityDepartment of Infectious Diseases, The Second Affiliated Hospital of Anhui Medical UniversityAbstract Bandavirus dabieense (DBV) causes severe fever with thrombocytopenia syndrome, which has a mortality rate of 6.18% of 27%. DBV has been classified into five to six genotypes using phylogenetic analyses. However, the absence of clear standards poses challenges in identifying new genotypes. We performed evolutionary and homology analyses using the sequences from GenBank and analysed nucleotide differences between the different genotypes. Nucleotide differences within the same genotype were mostly below 3%, whereas those between different genotypes ranged from 3 to 7%. Consequently, we established and validated a specific genotyping criterion for DBV using phylogenetic tree analysis with a 3% cut-off value and identified 8, 11, and 11 genotypes in the S, M, and L segments, respectively. Furthermore, we compared our method with the previous genotyping methods to elucidate the convenience and advantages of using a 3% cut-off value. Importantly, we also identified new genotypes and fragment reassortments in DBV using our new genotyping criterion. Additionally, we established two simplified genotyping methods for the rapid typing of DBV in clinical settings and demonstrated the existence of geographical and clinical variations among the different genotypes. Our findings provide a more reliable foundation for clinical typing of DBV.https://doi.org/10.1038/s41598-025-94203-xBandavirus dabieenseGenotyping criteriaPhylogenetic analysisCut-off valueReassortment |
| spellingShingle | Bingyan Liu Tengfei He Changtai Wang Guomei Xia Shijun Zhou Shanshan Sun Xinlei Chen Xiaodan Hong Jie Zhu Zhenhua Zhang Establishment of a genotyping criteria for Bandavirus dabieense and confirmation of new genotypes Scientific Reports Bandavirus dabieense Genotyping criteria Phylogenetic analysis Cut-off value Reassortment |
| title | Establishment of a genotyping criteria for Bandavirus dabieense and confirmation of new genotypes |
| title_full | Establishment of a genotyping criteria for Bandavirus dabieense and confirmation of new genotypes |
| title_fullStr | Establishment of a genotyping criteria for Bandavirus dabieense and confirmation of new genotypes |
| title_full_unstemmed | Establishment of a genotyping criteria for Bandavirus dabieense and confirmation of new genotypes |
| title_short | Establishment of a genotyping criteria for Bandavirus dabieense and confirmation of new genotypes |
| title_sort | establishment of a genotyping criteria for bandavirus dabieense and confirmation of new genotypes |
| topic | Bandavirus dabieense Genotyping criteria Phylogenetic analysis Cut-off value Reassortment |
| url | https://doi.org/10.1038/s41598-025-94203-x |
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