<i>miR-192-2</i> Regulates the Proliferation and Apoptosis of Ovarian Granulosa Cells by Targeting <i>IGFBP2</i> in Zhedong White Geese
The proliferation and apoptosis of ovarian granulosa cells (GC) is critical for follicular development and ovulation, especially for egg-laying performance in female birds. In geese, follicular atrophy is accompanied by follicular GC apoptosis during brooding stages. MicroRNAs are involved in follic...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/15/5/663 |
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| Summary: | The proliferation and apoptosis of ovarian granulosa cells (GC) is critical for follicular development and ovulation, especially for egg-laying performance in female birds. In geese, follicular atrophy is accompanied by follicular GC apoptosis during brooding stages. MicroRNAs are involved in follicular development, atrophy, ovulation, and degeneration. Our previous high-throughput sequencing study of ovaries from laying and brooding geese revealed that <i>miR-192-2</i> may be involved in follicle growth and development, as well as follicle cell proliferation and apoptosis in geese. To further investigate the effect of <i>miR-192-2</i> on GC in geese, we screened the target gene of <i>miR-192-2</i> (<i>IGFBP2</i>) and constructed a <i>miR-192-2</i> overexpression and interference vector, synthesized a small interfering RNA for <i>IGFBP2</i>. The results showed that after <i>miR-192-2</i> overexpression, the mRNA and protein expression of proliferation-related genes (<i>PCNA</i>, <i>CDK2</i>, <i>CCND1</i>, and <i>CCND2</i>) were significantly decreased, the mRNA and protein expression of apoptosis-related genes (<i>Caspase3</i>, <i>Caspase8</i>, and <i>Caspase9</i>) were significantly increased, and the mRNA and protein expression of anti-apoptosis gene <i>Bcl-2</i> were significantly decreased. While the mRNA and protein expressions of <i>PCNA</i>, <i>CDK2</i>, <i>CCND1</i>, and <i>CCND2</i> were significantly decreased after the downregulation of <i>IGFBP2</i> expression, the mRNA and protein expressions of <i>Caspase3</i>, <i>Caspase8</i>, and <i>Caspase9</i> were significantly increased, and the mRNA and protein expression of <i>Bcl-2</i> was significantly decreased. In conclusion, <i>miR-192-2</i> inhibited proliferation and promoted apoptosis of follicular GC by targeting <i>IGFBP2</i> in Zhedong White geese. Apoptosis of GC leads to follicular atresia, which in turn leads to brooding behavior in female geese. Therefore, it is possible to explore inhibiting the expression of <i>miR-192-2</i> in production to alter the brooding behavior of Zhedong White Geese, thereby improving egg production and economic returns for producers. |
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| ISSN: | 2076-2615 |